FastQCFastQC Report
Tue 18 Jul 2023
SRR10530139.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10530139.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30873245
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTG430918813.957677594305359No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT20647966.687978539346933No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA14831874.804117610571872No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGC10822693.505523957718083No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA8169272.6460678169722684No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG6748092.1857404364199486No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTTTGGTGACTCTAGA5660941.8336070600936183No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAG3605971.167991897191241No Hit
AACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC3114891.0089286046866792RNA PCR Primer, Index 1 (100% over 31bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGG2653980.8596375275744419Illumina Small RNA Adapter 2 (100% over 21bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA1914850.6202295871392852No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA1911150.619031138450137No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATGGAATTCTCGGGTGC1890490.6123392600939745No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAA1777350.5756926426101305No Hit
TTTGGTGACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTA1372470.44454996551220965RNA PCR Primer, Index 2 (100% over 40bp)
TTTGGTGACTCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATG1325290.42926812520031504RNA PCR Primer, Index 2 (100% over 38bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCA1317210.42665097238725636No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTTTGGTGACTCTAGAT1278670.4141676717170482No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTTGGTGACTCTAGAT1238680.4012147087227145No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG1142760.3701457362191762Illumina Small RNA Adapter 2 (100% over 21bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATTTGGTGACTCTAGAT1060140.3433847008955489No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTCG1015380.32888671080736737No Hit
TTTGGTGACTCTAGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC886740.2872195650311459RNA PCR Primer, Index 2 (100% over 34bp)
AGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCC731630.2369786525517483RNA PCR Primer, Index 1 (100% over 27bp)
GGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGAT729770.23637618915666297RNA PCR Primer, Index 2 (100% over 37bp)
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCC715280.2316828049659179No Hit
GCCGCCTGGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGT698590.22627682966270632RNA PCR Primer, Index 2 (100% over 39bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTTTGGTGACTCTAGATAA693930.2247674321244819No Hit
CGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCT692530.22431396505291232RNA PCR Primer, Index 2 (100% over 43bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTTGGTGACTCTAGATAAC689930.22347181191999738No Hit
ATGTACACGGAGTCGACCCAACGCGATGGAATTCTCGGGTGCCAAGGAAC670520.2171848148777364RNA PCR Primer, Index 1 (100% over 24bp)
CGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTAT667870.21632646649226542RNA PCR Primer, Index 2 (100% over 41bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATTGGAATTCTCGGGTGC648260.20997468843978015No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCTGGAATTCTCGGGTGCC590660.19131775749520338No Hit
GGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATG573030.18560731144393794RNA PCR Primer, Index 2 (100% over 38bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTTTGGTGACTCTAG565190.18306789584314834No Hit
GCAGGACACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTA543460.1760294390822863RNA PCR Primer, Index 2 (100% over 40bp)
TGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCC529110.17138140159869816No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGGTGGAATTCTCGGGTGCCAAG497570.1611654362863379No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGATGGAATTCTCGGGTGC491320.15914102971683086No Hit
AACCTCGGGCCGATCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC485610.15729153187492925RNA PCR Primer, Index 1 (100% over 33bp)
TTTGGTGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTAT471370.15267912394696445RNA PCR Primer, Index 2 (100% over 41bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCATGGAATTCTCGGGTGCC463590.15015914264924207No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGA445880.14442278419388696RNA PCR Primer, Index 1 (100% over 22bp)
TTGGTGACTCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGT420790.13629600646125797RNA PCR Primer, Index 2 (100% over 39bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCATGGAATTCTCGGGTG420420.13617616159234314No Hit
GGAATAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGT362690.11747712299112062RNA PCR Primer, Index 2 (100% over 39bp)
GAATACAAGCTTGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC343920.11139742518157711RNA PCR Primer, Index 2 (100% over 34bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAGTGGGGGGCCCAAGTC339920.11010180497709261No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG327580.10610481664625795No Hit
GAATACAAGCTTGGGCTGCAGGTCTGGAATTCTCGGGTGCCAAGGAACTC318760.10324797409536964RNA PCR Primer, Index 1 (100% over 26bp)
CTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATG317820.10294350334731578RNA PCR Primer, Index 2 (100% over 38bp)
AACCTCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTA317250.10275887746817673RNA PCR Primer, Index 2 (100% over 40bp)
ATGTACACGGAGTCGAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT312700.10128510948557562RNA PCR Primer, Index 1 (100% over 30bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATTCTCGGGTGCCAAGGA312530.10123004562688502RNA PCR Primer, Index 1 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)