FastQCFastQC Report
Tue 18 Jul 2023
SRR10530140.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10530140.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26914357
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTG22706098.436422984208763No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA11240554.176414097501939No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGC9524993.538999649889462No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT6530652.4264558874655635No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA6185902.2983644008288957No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA5958512.213877894240609No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG4546551.689265695628545No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAG3013781.1197666732294589No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTTTGGTGACTCTAGA2682060.9965164688868473No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGG2529930.9399927332464231Illumina Small RNA Adapter 2 (100% over 21bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA2096140.7788185316855238No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATGGAATTCTCGGGTGC1874330.6964052680136479No Hit
AACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1655150.6149691779744172RNA PCR Primer, Index 1 (100% over 31bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG1616070.6004490465813469Illumina Small RNA Adapter 2 (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG1552660.5768891302140341No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAA1426280.5299327789996989No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCA1198460.44528650638021927No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTTGGTGACTCTAGAT1079590.40112048747811435No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAG905970.3366121657671406No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATTTGGTGACTCTAGAT892880.3317485905384996No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTTTGGTGACTCTAGAT883160.32813713513571957No Hit
TTTGGTGACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCA685760.2547933803508663RNA PCR Primer, Index 3 (100% over 40bp)
CGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCT683750.2540465670422667RNA PCR Primer, Index 3 (100% over 43bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTCG682760.2536787336216132No Hit
TTTGGTGACTCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGG656950.24408905626093913RNA PCR Primer, Index 3 (100% over 38bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCATGGAATTCTCGGGTGCC609770.22655937869888548No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCTGGAATTCTCGGGTGCC602050.22369102111560757No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTTGGTGACTCTAGATAAC593810.22062945809925905No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGA579440.21529030026613674RNA PCR Primer, Index 1 (100% over 22bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCCGCCGAGGGCGCAC532010.1976677354766454No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTTTGGTGACTCTAGATAA497130.18470810950452948No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGGTGGAATTCTCGGGTGCCAAG487150.18100005138521422No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCC463620.17225750553877248No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATTGGAATTCTCGGGTGC433670.16112961569172912No Hit
AGGTCGGTTTCTATCTACTTCAAATTCCTTGGAATTCTCGGGTGCCAAGG408590.15181116903517333Illumina Small RNA Adapter 2 (100% over 21bp)
GCCGCCTGGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGC401960.14934779976352397RNA PCR Primer, Index 3 (100% over 39bp)
CGTTCCCGTGGTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGA396770.14741946092191613No Hit
CTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGG392250.14574005984984148RNA PCR Primer, Index 3 (100% over 38bp)
AACCTCGGGCCGATCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC390160.14496352262846182RNA PCR Primer, Index 1 (100% over 33bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAGTGGGGGGCCCAAGTC380450.14135578271477933No Hit
GCAGGACACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCA366070.13601290939255953RNA PCR Primer, Index 3 (100% over 40bp)
TGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCC348100.1293361754843335No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTTGGTGACTCTAGATA341180.1267650570288564No Hit
GGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAG318970.118512955743286RNA PCR Primer, Index 3 (100% over 37bp)
TTTGGTGACTCTAGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT314090.116699797063701RNA PCR Primer, Index 3 (100% over 34bp)
TTCCCGTGGTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAAT314040.11668121961821343No Hit
AGCCTGGTGATAGCTGGTTGTCCAAGATAGAATTGGAATTCTCGGGTGCC312390.11606816391712424No Hit
CGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC311910.11588982044044374RNA PCR Primer, Index 3 (100% over 44bp)
TTGGTGACTCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGC309590.11502782696982135RNA PCR Primer, Index 3 (100% over 39bp)
CGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCAT306190.11376456067666785RNA PCR Primer, Index 3 (100% over 41bp)
CGATCAAAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCA299600.11131605336140857No Hit
GAATACAAGCTTGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT291580.10833623110520531RNA PCR Primer, Index 3 (100% over 34bp)
GGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGG286460.10643390068728004RNA PCR Primer, Index 3 (100% over 38bp)
GAATACAAGCTTGGGCTGCAGGTCTGGAATTCTCGGGTGCCAAGGAACTC286410.10641532324179247RNA PCR Primer, Index 1 (100% over 26bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCATGGAATTCTCGGGTG280630.10426777054343152No Hit
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCG275740.102450896374749RNA PCR Primer, Index 3 (100% over 45bp)
AAAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAA273430.1015926183932241RNA PCR Primer, Index 1 (100% over 23bp)
CCGCCTGGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCA272070.10108731187596272RNA PCR Primer, Index 3 (100% over 40bp)
GTTCCCGTGGTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAA270600.10054113497862868No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)