FastQCFastQC Report
Tue 18 Jul 2023
SRR10530141.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10530141.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16085080
Total Bases804.2 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTG12129787.541013162508362No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGC6103143.794286382162849No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA5363823.334655469540717No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT2281931.418662512091951No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA2242871.3943791389287463No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG2203701.3700273794099873No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA1937731.2046753886210078No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA1770171.100504318287506No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG1201490.7469592939543975No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAG1155190.7181748552074344No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCA1124300.698970723179493No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATGGAATTCTCGGGTGC1116520.694133942759377No Hit
AACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1058650.6581565027963803RNA PCR Primer, Index 1 (100% over 31bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTTTGGTGACTCTAGA890210.5534383416184439No Hit
ATGTACACGGAGTCGACCCAACGCGATGGAATTCTCGGGTGCCAAGGAAC794260.4937867887508175RNA PCR Primer, Index 1 (100% over 24bp)
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGATGGAATTCTCGGGTGC770560.47905263759956435No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGG767150.4769326605773798Illumina Small RNA Adapter 2 (100% over 21bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATGC737470.45848077846053614RNA PCR Primer, Index 4 (100% over 50bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAG698780.4344274321296505No Hit
GCCGCCTGGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCA628230.39056691045366265RNA PCR Primer, Index 4 (100% over 39bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTCG615770.38282060145178015No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTTGGTGACTCTAGATAAC589570.3665322149470192No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATTGGAATTCTCGGGTGC579780.360445829302683No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTTTGGTGACTCTAGATAA527690.32806178147699605No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG518120.3221121685437685Illumina Small RNA Adapter 2 (100% over 21bp)
CGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCT464950.2890566910453663RNA PCR Primer, Index 4 (100% over 43bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAA436610.2714378790780027No Hit
CCGCCTGGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAA389550.24218095278357335RNA PCR Primer, Index 4 (100% over 40bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCATGGAATTCTCGGGTGCC387150.24068888684420595No Hit
TTTGGTGACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAA366070.2275835743434288RNA PCR Primer, Index 4 (100% over 40bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTTTGGTGACTCTAGAT361640.22482946929701314No Hit
AGGTCGGTTTCTATCTACTTCAAATTCCTTGGAATTCTCGGGTGCCAAGG359040.22321306452936512Illumina Small RNA Adapter 2 (100% over 21bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTTGGTGACTCTAGAT339610.2111335473619031No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATTTGGTGACTCTAGAT335550.20860946914780654No Hit
AACCTCGGGCCGATCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC331430.20604808928522583RNA PCR Primer, Index 1 (100% over 33bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCTGGAATTCTCGGGTGCC311580.1937074605783745No Hit
ATGTACACGGAGTCGACCCAACGCGATTTGGTGACTCTAGATAACCTCGG301020.18714237044515789No Hit
TTTGGTGACTCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACC296370.18425149268763352RNA PCR Primer, Index 4 (100% over 38bp)
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCC287570.17878058424328633No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATTGGAATTCTCGGGTGCC276110.17165596938280694No Hit
ACATATACTAAAATTGGAACGATACAGAGAATGGAATTCTCGGGTGCCAA274900.17090371947170918No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG270410.1681123127768093No Hit
CGACTGCATAATTTGTGGTAGTGGGGTGGAATTCTCGGGTGCCAAGGAAC263450.1637853215526438RNA PCR Primer, Index 1 (100% over 24bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCCGCCGAGGGCGCAC257570.16012976000119367No Hit
TTTGGTGACTCTAGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT243840.15159389943972926RNA PCR Primer, Index 3 (100% over 34bp)
GAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC222240.1381653059854225RNA PCR Primer, Index 4 (100% over 44bp)
CGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCG213380.13265709589259114RNA PCR Primer, Index 4 (100% over 45bp)
AGGGCTTCTGTCGTGAGTGGCACACGTAGGTGGAATTCTCGGGTGCCAAG213020.13243328600168602No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCC212790.1322902963491633No Hit
GCAGGACACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAA195800.12172771288672483RNA PCR Primer, Index 4 (100% over 40bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGTGGAATTCTCGG189750.11796646333123616No Hit
CTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACC189690.11792916168275197RNA PCR Primer, Index 4 (100% over 38bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGA184450.11467148438179978RNA PCR Primer, Index 1 (100% over 22bp)
CGCCTGGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAAT183920.11434198648685615RNA PCR Primer, Index 4 (100% over 41bp)
CGTTCCCGTGGTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGA181890.11307994737980787No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAA171360.10653350807083334No Hit
ACGATCAAAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCC170480.10598641722639862No Hit
GGAATAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCA169960.10566313627286901RNA PCR Primer, Index 4 (100% over 39bp)
CGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC169800.10556366521024452RNA PCR Primer, Index 4 (100% over 44bp)
CGATCAAAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCA169680.10548906191327614No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGTGGAATTCTCGGGTGCCAA162540.10105016574365809No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)