FastQCFastQC Report
Tue 18 Jul 2023
SRR10530142_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10530142_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26112342
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGA323593112.392343053717664RNA PCR Primer, Index 1 (100% over 22bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGG8065593.0888037541787714Illumina Small RNA Adapter 2 (100% over 21bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTG7265712.7824811730789984No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAG6881482.635336194662279No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG6674132.555929299639228Illumina Small RNA Adapter 2 (100% over 21bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGC5491492.1030246923083347No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAA4884181.8704488475219878No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGTGGAATTCTCGGGT4631871.7738240407543682No Hit
AGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAACTC4318981.6539994765693555RNA PCR Primer, Index 1 (100% over 26bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG3094321.1850028618650905No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA2772371.0617086740055717No Hit
AAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAACT2763501.058311812858456RNA PCR Primer, Index 1 (100% over 25bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATGGAATTCTCGGGTGC2548920.9761361121878689No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGC2480480.9499262839005403RNA PCR Primer, Index 1 (100% over 50bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA2162160.8280222432748467RNA PCR Primer, Index 1 (100% over 23bp)
ATGTACACGGAGTCGACCCAACGCGATGGAATTCTCGGGTGCCAAGGAAC1972680.7554588554331894RNA PCR Primer, Index 1 (100% over 24bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCATGGAATTCTCGGGTGCC1871860.7168487606358709No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGA1840900.7049922982779561RNA PCR Primer, Index 1 (100% over 22bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA1805250.6913397503755121No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGATGGAATTCTCGGGTGC1780970.6820414652963721No Hit
ACATATACTAAAATTGGAACGATACAGAGAATGGAATTCTCGGGTGCCAA1684450.6450781013820974No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCTGGAATTCTCGGGTG1460330.5592489559151761No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAA1132170.43357658229200585RNA PCR Primer, Index 1 (100% over 23bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC880270.337108789399281RNA PCR Primer, Index 1 (100% over 24bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCA750170.28728560617044613No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT737350.28237605037495295No Hit
GGAATAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACG729580.27940044596536RNA PCR Primer, Index 1 (100% over 39bp)
ATTGATCATCGACACTTCGAACGCATGGAATTCTCGGGTGCCAAGGAACT720030.275743171562321RNA PCR Primer, Index 1 (100% over 25bp)
TGCATTTATCAGTTGGTGACTCTAGATAACCTTGGAATTCTCGGGTGCCA694820.266088733059639No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA691140.26467943779229No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAA677360.2594022397531405No Hit
AAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC654490.2506439292193707RNA PCR Primer, Index 1 (100% over 31bp)
GCAGGACACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGA645950.24737344509351172RNA PCR Primer, Index 1 (100% over 40bp)
CTAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC627650.24036526482381398RNA PCR Primer, Index 1 (100% over 24bp)
TAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACT621310.23793729417300064RNA PCR Primer, Index 1 (100% over 25bp)
CCGCCTGGATACCGCAGCTAGGAATAATGGAATTGGAATTCTCGGGTGCC615580.23574292953117723No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAA607680.23271754023442248RNA PCR Primer, Index 1 (100% over 23bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA602290.2306533822205607RNA PCR Primer, Index 1 (100% over 22bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG563550.21581748584634805No Hit
GCATTTATCAGTTGGTGACTCTAGATAACCTTGGAATTCTCGGGTGCCAA560750.21474519596901726No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGG524370.2008130867771263Illumina Small RNA Adapter 2 (100% over 21bp)
AAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAAC519910.19910508218680653RNA PCR Primer, Index 1 (100% over 24bp)
CGCCGCGACCGGCTCCGGGACGGCTGGGTGGAATTCTCGGGTGCCAAGGA511150.19575034671344302RNA PCR Primer, Index 1 (100% over 22bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCTGGAATTCTCGGGTGCC475660.18215907251827507No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAGGGGGGCCCAAGTCCT470170.18005661843736576No Hit
GGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACT467050.17886178114548287RNA PCR Primer, Index 1 (100% over 25bp)
GAATACAAGCTTGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA407280.15597222187117493RNA PCR Primer, Index 1 (100% over 34bp)
CGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGTGGAATTCTCGGGTGCCA407230.15595307383765117No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCCTGGAATTCTCGGGT405170.1551641748564721No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGTGGAATTCTCGGGTGCCAAG402100.15398848559811296No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG401300.1536821170617327No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCTGGAATTCTCGGGTGCCA399230.15288938847384886No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGA372950.14282518205375833RNA PCR Primer, Index 1 (100% over 22bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTTGGAATTCTCGGGTGCC372110.1425034950905591No Hit
TTAACCAGTGAAATTGACCTGCCCGTGATGGAATTCTCGGGTGCCAAGGA368830.14124738409140014RNA PCR Primer, Index 1 (100% over 22bp)
GAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC359130.13753266558778987RNA PCR Primer, Index 1 (100% over 44bp)
GGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAAC355700.13621911048805965RNA PCR Primer, Index 1 (100% over 24bp)
ATGTACACGGAGTCGAGCTCAACCTGGAATTCTCGGGTGCCAAGGAACTC352830.13512001336379556RNA PCR Primer, Index 1 (100% over 26bp)
ATTGATCATCGACACTTCGAACGCACTGGAATTCTCGGGTGCCAAGGAAC352480.13498597712912921RNA PCR Primer, Index 1 (100% over 24bp)
TGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCC347890.13322818765164765No Hit
ATGTACACGGAGTCGACCCAACGCGATTTGGTGACTCTAGATAACCTCGG336990.12905391634346702No Hit
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCG334860.12823821011535466RNA PCR Primer, Index 1 (100% over 45bp)
CGCCTGGATACCGCAGCTAGGAATAATGGAATTGGAATTCTCGGGTGCCA334000.1279088639387459No Hit
GAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCG329380.12613958564115008RNA PCR Primer, Index 1 (100% over 45bp)
TGAGTCTGAAACCAATTTTTTGAGGCCTTGGAATTCTCGGGTGCCAAGGA325200.12453881003856336RNA PCR Primer, Index 1 (100% over 22bp)
ATGTACACGGAGTCGAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT316660.12126832591270442RNA PCR Primer, Index 1 (100% over 30bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAGG310910.11906630205747151Illumina Small RNA Adapter 2 (100% over 21bp)
TACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG307720.11784465751865536RNA PCR Primer, Index 1 (100% over 29bp)
AAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTC289610.1109092397763479RNA PCR Primer, Index 1 (100% over 26bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACTGGAATTCTCGGGTGCC289300.11079052196850056No Hit
ATGTACACGGAGTCGACCCAACGCGATTGGTGACTCTAGATAACCTCGGG280780.10752769705605111No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAGGGGGGCCCAAGTCCTT278150.10652051049270111No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGGAATTCTCGGGTGCCA264420.1012624604870754No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)