FastQCFastQC Report
Tue 18 Jul 2023
SRR10530143_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10530143_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26978513
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGA342592612.698720644833166RNA PCR Primer, Index 1 (100% over 22bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGG8561633.1734995920642475Illumina Small RNA Adapter 2 (100% over 21bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTG7714792.859605345928443No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAG7355632.7264771783381834No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG7011392.5988793377900405Illumina Small RNA Adapter 2 (100% over 21bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGC5735222.1258473363598656No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAA5176751.9188418575923734No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGTGGAATTCTCGGGT4706641.7445883692700188No Hit
AGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAACTC4498741.6675270427247046RNA PCR Primer, Index 1 (100% over 26bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG3250901.204995990698227No Hit
AAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAACT2931481.0865980641705493RNA PCR Primer, Index 1 (100% over 25bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA2919451.0821389599938291No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATGGAATTCTCGGGTGC2694160.9986317629885679No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGC2399100.889263244419735RNA PCR Primer, Index 1 (100% over 50bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA2163590.8019678475236941RNA PCR Primer, Index 1 (100% over 23bp)
ATGTACACGGAGTCGACCCAACGCGATGGAATTCTCGGGTGCCAAGGAAC2024680.7504787235679001RNA PCR Primer, Index 1 (100% over 24bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCATGGAATTCTCGGGTGCC1950980.7231606871735295No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA1888710.7000793557450702No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGATGGAATTCTCGGGTGC1881690.6974772849786051No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGA1829610.6781730334803849RNA PCR Primer, Index 1 (100% over 22bp)
ACATATACTAAAATTGGAACGATACAGAGAATGGAATTCTCGGGTGCCAA1764680.654105732217339No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCTGGAATTCTCGGGTG1495690.5544004593581566No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAA1190180.4411584878677339RNA PCR Primer, Index 1 (100% over 23bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC922020.3417608672501705RNA PCR Primer, Index 1 (100% over 24bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT773690.28678007568467545No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCA757170.28065668408040134No Hit
GGAATAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACG757020.2806010842776991RNA PCR Primer, Index 1 (100% over 39bp)
ATTGATCATCGACACTTCGAACGCATGGAATTCTCGGGTGCCAAGGAACT742450.2752004901085542RNA PCR Primer, Index 1 (100% over 25bp)
TGCATTTATCAGTTGGTGACTCTAGATAACCTTGGAATTCTCGGGTGCCA737660.27342500307559575No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA697090.25838710977139473No Hit
AAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC684140.2535869934714341RNA PCR Primer, Index 1 (100% over 31bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAA679780.25197089253955546No Hit
GCAGGACACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGA655000.24278580513314427RNA PCR Primer, Index 1 (100% over 40bp)
CCGCCTGGATACCGCAGCTAGGAATAATGGAATTGGAATTCTCGGGTGCC652180.24174052884234207No Hit
TAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACT639900.2371887583277848RNA PCR Primer, Index 1 (100% over 25bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAA626330.2321588295099882RNA PCR Primer, Index 1 (100% over 23bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA605040.22426736417978263RNA PCR Primer, Index 1 (100% over 22bp)
GCATTTATCAGTTGGTGACTCTAGATAACCTTGGAATTCTCGGGTGCCAA591480.2192411420154995No Hit
CTAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC585120.21688371038092427RNA PCR Primer, Index 1 (100% over 24bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG560280.2076763830534322No Hit
AAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAAC554160.20540791110318052RNA PCR Primer, Index 1 (100% over 24bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAGGGGGGCCCAAGTCCT516250.191355987633566No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCTGGAATTCTCGGGTGCC513750.19042932425519524No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGG512150.18983625969303794Illumina Small RNA Adapter 2 (100% over 21bp)
CGCCGCGACCGGCTCCGGGACGGCTGGGTGGAATTCTCGGGTGCCAAGGA499920.1853030224460481RNA PCR Primer, Index 1 (100% over 22bp)
GGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACT494140.18316057671525485RNA PCR Primer, Index 1 (100% over 25bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG427550.15847797096897076No Hit
GAATACAAGCTTGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA423900.1571250424365494RNA PCR Primer, Index 1 (100% over 34bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCTGGAATTCTCGGGTGCCA423170.15685445673006515No Hit
CGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGTGGAATTCTCGGGTGCCA416520.15438953214359888No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGTGGAATTCTCGGGTGCCAAG410600.15219519326361688No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCCTGGAATTCTCGGGT410560.15218036664956291No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGA396760.14706518480095623RNA PCR Primer, Index 1 (100% over 22bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTTGGAATTCTCGGGTGCC389160.14424812813070906No Hit
TTAACCAGTGAAATTGACCTGCCCGTGATGGAATTCTCGGGTGCCAAGGA388500.14400348899881915RNA PCR Primer, Index 1 (100% over 22bp)
GGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAAC373030.1382692960134608RNA PCR Primer, Index 1 (100% over 24bp)
ATGTACACGGAGTCGACCCAACGCGATTTGGTGACTCTAGATAACCTCGG372850.1382025762502181No Hit
ATTGATCATCGACACTTCGAACGCACTGGAATTCTCGGGTGCCAAGGAAC365160.13535215969834957RNA PCR Primer, Index 1 (100% over 24bp)
ATGTACACGGAGTCGAGCTCAACCTGGAATTCTCGGGTGCCAAGGAACTC363530.13474797517565182RNA PCR Primer, Index 1 (100% over 26bp)
TGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCC362130.1342290436837642No Hit
CGCCTGGATACCGCAGCTAGGAATAATGGAATTGGAATTCTCGGGTGCCA358460.13286870184431587No Hit
GAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC357190.13239795684810354RNA PCR Primer, Index 1 (100% over 44bp)
TGAGTCTGAAACCAATTTTTTGAGGCCTTGGAATTCTCGGGTGCCAAGGA344330.12763120042976422RNA PCR Primer, Index 1 (100% over 22bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCG336170.12460657116276201RNA PCR Primer, Index 1 (100% over 45bp)
GAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCG334250.12389489368817325RNA PCR Primer, Index 1 (100% over 45bp)
ATGTACACGGAGTCGAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT332650.12330182912601595RNA PCR Primer, Index 1 (100% over 30bp)
TACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG325430.12062562528928114RNA PCR Primer, Index 1 (100% over 29bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAGG310920.11524727104121714Illumina Small RNA Adapter 2 (100% over 21bp)
ATGTACACGGAGTCGACCCAACGCGATTGGTGACTCTAGATAACCTCGGG308290.11427242116717107No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACTGGAATTCTCGGGTGCC307800.1140907951450104No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAGGGGGGCCCAAGTCCTT304510.11287130613907446No Hit
AAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTC301490.11175189677800254RNA PCR Primer, Index 1 (100% over 26bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGGAATTCTCGGGTGCCA277900.10300790113969588No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATTCTCGGGTGCCAAGGAA272460.10099148162836105RNA PCR Primer, Index 1 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)