FastQCFastQC Report
Tue 18 Jul 2023
SRR10530144_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10530144_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24917272
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGA638224525.613738935787193RNA PCR Primer, Index 1 (100% over 22bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG22952839.211614337235634Illumina Small RNA Adapter 2 (100% over 21bp)
AGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAACTC11105204.456828179264568RNA PCR Primer, Index 1 (100% over 26bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAG8829113.5433694346636337No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGG7662543.0751921799465043Illumina Small RNA Adapter 2 (100% over 21bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAA6079962.4400584462055073No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA4091731.642125991962523No Hit
AAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAACT3644531.462652091288324RNA PCR Primer, Index 1 (100% over 25bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGC2400000.9631873023660054RNA PCR Primer, Index 2 (100% over 50bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG2350960.9435061751543267No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA2182020.8757058156286129No Hit
TTTTTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGGAAC1940120.7786245621109726RNA PCR Primer, Index 1 (100% over 24bp)
TAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACT1723340.6916246690247633RNA PCR Primer, Index 1 (100% over 25bp)
CTAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC1515800.6083330470526629RNA PCR Primer, Index 1 (100% over 24bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAA1489240.5976737742398124RNA PCR Primer, Index 1 (100% over 23bp)
AAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTC1000340.4014644941870041RNA PCR Primer, Index 1 (100% over 26bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA923650.3706866465959837RNA PCR Primer, Index 1 (100% over 23bp)
AACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTCC917480.36821045257281776RNA PCR Primer, Index 1 (100% over 27bp)
TTTTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGGAACT895010.359192611454416RNA PCR Primer, Index 1 (100% over 25bp)
ATTGATCATCGACACTTCGAACGCATGGAATTCTCGGGTGCCAAGGAACT805560.3232938180391497RNA PCR Primer, Index 1 (100% over 25bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGA628860.25237915290245255RNA PCR Primer, Index 1 (100% over 22bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCGGGGGGCCCAAGTCCTTCTGAT626250.25133168671112954No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC622690.24990295887928662RNA PCR Primer, Index 1 (100% over 24bp)
AAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC619390.24857857633853336RNA PCR Primer, Index 1 (100% over 31bp)
ACATATACTAAAATTGGAACGATACAGAGAATGGAATTCTCGGGTGCCAA596840.23952862897671945No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGTGGAATTCTCGGGTGCCAAGGA570300.22887738272472205RNA PCR Primer, Index 1 (100% over 22bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGA523840.21023168186308677RNA PCR Primer, Index 1 (100% over 22bp)
TTTTTTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGGAA523130.2099467389528035RNA PCR Primer, Index 1 (100% over 23bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGTGGAATTCTCGGGT482090.19347623608234482No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCTGGAATTCTCGGGTG463800.18613594618223053No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTTGGAATTCTCGGGTGCC399960.1605151639392948No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG390190.15659418896257984No Hit
ATTGATCATCGACACTTCGAACGCACTGGAATTCTCGGGTGCCAAGGAAC332760.13354591947304664RNA PCR Primer, Index 1 (100% over 24bp)
GGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAAC305700.12268598263886993RNA PCR Primer, Index 1 (100% over 24bp)
GGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACT302540.12141778602408804RNA PCR Primer, Index 1 (100% over 25bp)
GGGGGGCCTAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGA296610.1190379107311587RNA PCR Primer, Index 1 (100% over 22bp)
GGAATAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGT283340.11371228760515999RNA PCR Primer, Index 2 (100% over 39bp)
ACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA266030.10676529918684519RNA PCR Primer, Index 1 (100% over 28bp)
GAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCG262620.10539677056140014RNA PCR Primer, Index 2 (100% over 45bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA262430.10532051823329616RNA PCR Primer, Index 1 (100% over 22bp)
GCAGGACACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTA259650.1042048262747222RNA PCR Primer, Index 2 (100% over 40bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGGAATTCTCGGGTGCCA249880.10028385129800725No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)