FastQCFastQC Report
Tue 18 Jul 2023
SRR10530145_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10530145_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26301325
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGA690070526.2371002221371RNA PCR Primer, Index 1 (100% over 22bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG24809469.432779527267163Illumina Small RNA Adapter 2 (100% over 21bp)
AGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAACTC11609604.414074195881766RNA PCR Primer, Index 1 (100% over 26bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAG9576173.64094584588419No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGG8269223.1440317170332674Illumina Small RNA Adapter 2 (100% over 21bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAA6550102.490406852126271No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA4456161.6942720566359297No Hit
AAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAACT3810881.4489308048168676RNA PCR Primer, Index 1 (100% over 25bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG2547210.9684721207011434No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGC2367780.9002512230847686RNA PCR Primer, Index 2 (100% over 50bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA2322770.8831380168109402No Hit
TTTTTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGGAAC2048570.7788847139830407RNA PCR Primer, Index 1 (100% over 24bp)
TAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACT1821880.6926951398836371RNA PCR Primer, Index 1 (100% over 25bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAA1618080.6152085493791662RNA PCR Primer, Index 1 (100% over 23bp)
CTAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC1495830.5687280013459398RNA PCR Primer, Index 1 (100% over 24bp)
AAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTC1061360.4035386049942351RNA PCR Primer, Index 1 (100% over 26bp)
AACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTCC972630.3698026620331865RNA PCR Primer, Index 1 (100% over 27bp)
TTTTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGGAACT934850.3554383666982557RNA PCR Primer, Index 1 (100% over 25bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA931050.3539935725671615RNA PCR Primer, Index 1 (100% over 23bp)
ATTGATCATCGACACTTCGAACGCATGGAATTCTCGGGTGCCAAGGAACT861130.32740936055502906RNA PCR Primer, Index 1 (100% over 25bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCGGGGGGCCCAAGTCCTTCTGAT705920.2683971244794701No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGA681760.2592112754775662RNA PCR Primer, Index 1 (100% over 22bp)
AAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC656960.24978209272726754RNA PCR Primer, Index 1 (100% over 31bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC650860.24746281793787955RNA PCR Primer, Index 1 (100% over 24bp)
ACATATACTAAAATTGGAACGATACAGAGAATGGAATTCTCGGGTGCCAA641410.2438698430592375No Hit
TTTTTTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGGAA562530.21387895856957778RNA PCR Primer, Index 1 (100% over 23bp)
CGCCGCGACCGGCTCCGGGACGGCTGGGTGGAATTCTCGGGTGCCAAGGA541610.20592498666892256RNA PCR Primer, Index 1 (100% over 22bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGA515560.19602054269129027RNA PCR Primer, Index 1 (100% over 22bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGTGGAATTCTCGGGT509020.19353397594988087No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCTGGAATTCTCGGGTG491720.18695636056358378No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTTGGAATTCTCGGGTGCC427810.16265720453247126No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG419840.15962693894699223No Hit
ATTGATCATCGACACTTCGAACGCACTGGAATTCTCGGGTGCCAAGGAAC355200.1350502303591169RNA PCR Primer, Index 1 (100% over 24bp)
GGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAAC337330.1282558958531557RNA PCR Primer, Index 1 (100% over 24bp)
GGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACT322190.12249953186769108RNA PCR Primer, Index 1 (100% over 25bp)
GGGGGGCCTAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGA321000.12204708317926947RNA PCR Primer, Index 1 (100% over 22bp)
GGAATAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGT294580.11200196187834642RNA PCR Primer, Index 2 (100% over 39bp)
ACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA282790.10751929798213589RNA PCR Primer, Index 1 (100% over 28bp)
GCAGGACACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTA269660.10252715405022371RNA PCR Primer, Index 2 (100% over 40bp)
GAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCG267680.10177434026612728RNA PCR Primer, Index 2 (100% over 45bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGGAATTCTCGGGTGCCA266370.10127626649988165No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA265820.10106715155985488RNA PCR Primer, Index 1 (100% over 22bp)
GCATTTATCAGTTGGTGACTCTAGATAACCTTGGAATTCTCGGGTGCCAA265290.10086564079946543No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)