FastQCFastQC Report
Tue 18 Jul 2023
SRR10530146_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10530146_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19678969
Total Bases983.9 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGA17443388.863970465119388RNA PCR Primer, Index 1 (100% over 22bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGC9998455.080779384326486RNA PCR Primer, Index 3 (100% over 50bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG7963174.046538210411328Illumina Small RNA Adapter 2 (100% over 21bp)
AGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAACTC6379783.2419279688890206RNA PCR Primer, Index 1 (100% over 26bp)
TTTTTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGGAAC4059062.0626385457490173RNA PCR Primer, Index 1 (100% over 24bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGG4050842.0584614976526465Illumina Small RNA Adapter 2 (100% over 21bp)
TCGCTTCTGGCGCCAAGCGCCCGGTGGAATTCTCGGGTGCCAAGGAACTC3422341.7390850099921393RNA PCR Primer, Index 1 (100% over 26bp)
TTTTTTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGGAA2470181.255238523928769RNA PCR Primer, Index 1 (100% over 23bp)
AAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAACT2452011.2460053166403178RNA PCR Primer, Index 1 (100% over 25bp)
TTTTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGGAACT2163021.0991531111208113RNA PCR Primer, Index 1 (100% over 25bp)
TAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACT2066181.0499432160292543RNA PCR Primer, Index 1 (100% over 25bp)
CGCTTCTGGCGCCAAGCGCCCGGTGGAATTCTCGGGTGCCAAGGAACTCC1811850.9207037218260774RNA PCR Primer, Index 1 (100% over 27bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG1737730.8830391470203546No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAG1726690.8774290970223084No Hit
CTAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC1478730.7514265610154679RNA PCR Primer, Index 1 (100% over 24bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA1440670.7320861169098848No Hit
AAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAAC1433270.7283257573097452RNA PCR Primer, Index 1 (100% over 24bp)
CGCTTCTGGCGCCAAGCGCCCGGCTGGAATTCTCGGGTGCCAAGGAACTC1356880.6895076667888445RNA PCR Primer, Index 1 (100% over 26bp)
AACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTCC1224940.6224614714317604RNA PCR Primer, Index 1 (100% over 27bp)
TTTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGGAACTC1190780.6051028384667917RNA PCR Primer, Index 1 (100% over 26bp)
AAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTC1134730.5766206552792477RNA PCR Primer, Index 1 (100% over 26bp)
CCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCA1062290.539809783734097RNA PCR Primer, Index 1 (100% over 28bp)
TTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGGAACTCC1022590.5196359626360507RNA PCR Primer, Index 1 (100% over 27bp)
TCGCTTCTGGCGCCAAGCGCCCGGCTGGAATTCTCGGGTGCCAAGGAACT1000560.5084412704750945RNA PCR Primer, Index 1 (100% over 25bp)
ATGTACACGGAGTCGACCCAACGCGATGGAATTCTCGGGTGCCAAGGAAC984860.5004632102423658RNA PCR Primer, Index 1 (100% over 24bp)
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGATGGAATTCTCGGGTGC914970.4649481382891553No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAA887880.451182173212428No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGTGGAATTCTCGGGTGCCAAGGAACT883260.4488344892458543RNA PCR Primer, Index 1 (100% over 25bp)
GCAGGACACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCA860350.4371926191865031RNA PCR Primer, Index 3 (100% over 40bp)
ACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA752820.3825505289428527RNA PCR Primer, Index 1 (100% over 28bp)
ACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT726140.3689929081142412RNA PCR Primer, Index 1 (100% over 30bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAGG553410.2812190008531443Illumina Small RNA Adapter 2 (100% over 21bp)
ATTGATCATCGACACTTCGAACGCATGGAATTCTCGGGTGCCAAGGAACT550250.27961322567254415RNA PCR Primer, Index 1 (100% over 25bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA518280.26336745588653554No Hit
ATGTACACGGAGTCGAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT510670.25950038337882436RNA PCR Primer, Index 1 (100% over 30bp)
CGTTTTTTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGG485070.24649157178915215Illumina Small RNA Adapter 2 (100% over 21bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAA458480.2329796850637856RNA PCR Primer, Index 1 (100% over 23bp)
CGGCCGAGGTGGGATCCCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCC422820.21485881704473442RNA PCR Primer, Index 1 (100% over 27bp)
GAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAGGAACT420030.21344105984414122RNA PCR Primer, Index 1 (100% over 25bp)
ATGTACACGGAGTCGAGCTCAACCTGGAATTCTCGGGTGCCAAGGAACTC390240.19830307167006564RNA PCR Primer, Index 1 (100% over 26bp)
AAAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAA388470.19740363430624844RNA PCR Primer, Index 1 (100% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG360590.18323622543437107No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGA352470.1791099930082719RNA PCR Primer, Index 1 (100% over 22bp)
AAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC345680.17565960899679245RNA PCR Primer, Index 1 (100% over 31bp)
AACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG320990.1631132200065969RNA PCR Primer, Index 1 (100% over 29bp)
TCGCTTCTGGCGCCAAGCGCCCGTGGAATTCTCGGGTGCCAAGGAACTCC301370.1531431854991997RNA PCR Primer, Index 1 (100% over 27bp)
CCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG270800.13760883509700128RNA PCR Primer, Index 1 (100% over 29bp)
CGCCGCGACCGGCTCCGGGACGGCTGGGTGGAATTCTCGGGTGCCAAGGA265040.13468185248932502RNA PCR Primer, Index 1 (100% over 22bp)
GGGAGAGCGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGC251370.12773535036312117RNA PCR Primer, Index 3 (100% over 39bp)
GGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAACTCC247020.12552486870628232RNA PCR Primer, Index 1 (100% over 27bp)
AGTCGAGCTCAACCCGCAACGCGATGGAATTCTCGGGTGCCAAGGAACTC245300.12465083917760121RNA PCR Primer, Index 1 (100% over 26bp)
ATGTACACGGAGTCGAGCTCAACCCTGGAATTCTCGGGTGCCAAGGAACT235640.11974204542931086RNA PCR Primer, Index 1 (100% over 25bp)
ATGTACACGGAGTCGAGCTCAACCCGTGGAATTCTCGGGTGCCAAGGAAC235300.11956927215038553RNA PCR Primer, Index 1 (100% over 24bp)
GAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAGGAAC231890.11783645779410497RNA PCR Primer, Index 1 (100% over 24bp)
GGGAGAGCCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGC225740.1147112940723673RNA PCR Primer, Index 3 (100% over 39bp)
GATTTATAGGTAGAGGCGACTGGAATTCTCGGGTGCCAAGGAACTCCAGT221170.11238901794092973RNA PCR Primer, Index 1 (100% over 30bp)
GGAATAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGC218390.11097634230736377RNA PCR Primer, Index 3 (100% over 39bp)
GGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACT213110.10829327491699388RNA PCR Primer, Index 1 (100% over 25bp)
GGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACTC212120.10779019978129953RNA PCR Primer, Index 1 (100% over 26bp)
ATTGATCATCGACACTTCGAACGCACTGGAATTCTCGGGTGCCAAGGAAC210460.1069466596547817RNA PCR Primer, Index 1 (100% over 24bp)
ATGTACACGGAGTCGAGCTCAACTGGAATTCTCGGGTGCCAAGGAACTCC210050.10673831540666587RNA PCR Primer, Index 1 (100% over 27bp)
GAATACAAGCTTGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT204770.10405524801629597RNA PCR Primer, Index 3 (100% over 34bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAG202630.10296779267247182No Hit
ATGTACACGGAGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTA200400.10183460322540272RNA PCR Primer, Index 3 (100% over 36bp)
GAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCG198710.10097581839780326RNA PCR Primer, Index 3 (100% over 45bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)