FastQCFastQC Report
Tue 18 Jul 2023
SRR10530147_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10530147_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20363735
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGA18650409.158634209294119RNA PCR Primer, Index 1 (100% over 22bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGC10006984.914118161525869RNA PCR Primer, Index 3 (100% over 50bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG8493284.170786940607899Illumina Small RNA Adapter 2 (100% over 21bp)
AGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAACTC6681373.2810140183026344RNA PCR Primer, Index 1 (100% over 26bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGG4307332.115196450945762Illumina Small RNA Adapter 2 (100% over 21bp)
TTTTTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGGAAC4251592.0878242621012304RNA PCR Primer, Index 1 (100% over 24bp)
TCGCTTCTGGCGCCAAGCGCCCGGTGGAATTCTCGGGTGCCAAGGAACTC3333701.6370768918373766RNA PCR Primer, Index 1 (100% over 26bp)
TTTTTTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGGAA2646101.299417813087825RNA PCR Primer, Index 1 (100% over 23bp)
AAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAACT2569981.2620376370051958RNA PCR Primer, Index 1 (100% over 25bp)
TTTTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGGAACT2248981.1044044719694104RNA PCR Primer, Index 1 (100% over 25bp)
TAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACT2150581.0560832774537676RNA PCR Primer, Index 1 (100% over 25bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAG1869900.9182500165121967No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG1838030.902599645890108No Hit
CGCTTCTGGCGCCAAGCGCCCGGTGGAATTCTCGGGTGCCAAGGAACTCC1785390.8767497711004392RNA PCR Primer, Index 1 (100% over 27bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA1530300.7514829671472351No Hit
AAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAAC1491210.732287077984466RNA PCR Primer, Index 1 (100% over 24bp)
CTAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC1442210.7082246945366358RNA PCR Primer, Index 1 (100% over 24bp)
CGCTTCTGGCGCCAAGCGCCCGGCTGGAATTCTCGGGTGCCAAGGAACTC1304230.6404669870237458RNA PCR Primer, Index 1 (100% over 26bp)
AACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTCC1297570.6371964671510408RNA PCR Primer, Index 1 (100% over 27bp)
TTTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGGAACTC1237120.6075113430812177RNA PCR Primer, Index 1 (100% over 26bp)
AAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTC1193020.5858551979781705RNA PCR Primer, Index 1 (100% over 26bp)
CCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCA1076590.5286800284918263RNA PCR Primer, Index 1 (100% over 28bp)
TTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGGAACTCC1053660.5174198151763416RNA PCR Primer, Index 1 (100% over 27bp)
ATGTACACGGAGTCGACCCAACGCGATGGAATTCTCGGGTGCCAAGGAAC1027290.5044703243290094RNA PCR Primer, Index 1 (100% over 24bp)
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGATGGAATTCTCGGGTGC976210.4793865172572713No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCTGGAATTCTCGGGTGCCAAGGAACT957690.4702919184520914RNA PCR Primer, Index 1 (100% over 25bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAA952810.4678955014883075No Hit
GCAGGACACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCA897490.4407295616447572RNA PCR Primer, Index 3 (100% over 40bp)
CTCGCTTCTGGCGCCAAGCGCCCGGTGGAATTCTCGGGTGCCAAGGAACT854370.4195546642106667RNA PCR Primer, Index 1 (100% over 25bp)
ACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA790950.388411065062475RNA PCR Primer, Index 1 (100% over 28bp)
ACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT773290.37973878564025704RNA PCR Primer, Index 1 (100% over 30bp)
ATTGATCATCGACACTTCGAACGCATGGAATTCTCGGGTGCCAAGGAACT572170.2809749783131631RNA PCR Primer, Index 1 (100% over 25bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAGG554820.2724549302964314Illumina Small RNA Adapter 2 (100% over 21bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA543340.26681745760293973No Hit
ATGTACACGGAGTCGAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT536580.2634978308252391RNA PCR Primer, Index 1 (100% over 30bp)
CGTTTTTTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGG515030.25291529279869335Illumina Small RNA Adapter 2 (100% over 21bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAA487280.23928812666242216RNA PCR Primer, Index 1 (100% over 23bp)
GAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAGGAACT447290.21965027535469306RNA PCR Primer, Index 1 (100% over 25bp)
CGGCCGAGGTGGGATCCCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCC436830.21451369309215623RNA PCR Primer, Index 1 (100% over 27bp)
AAAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAA410370.20152000603032794RNA PCR Primer, Index 1 (100% over 23bp)
ATGTACACGGAGTCGAGCTCAACCTGGAATTCTCGGGTGCCAAGGAACTC405160.19896153628005867RNA PCR Primer, Index 1 (100% over 26bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGA368180.1808018028126962RNA PCR Primer, Index 1 (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG367970.1806986783122055No Hit
AAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC363840.1786705631358884RNA PCR Primer, Index 1 (100% over 31bp)
AACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG340140.16703222665193787RNA PCR Primer, Index 1 (100% over 29bp)
TCGCTTCTGGCGCCAAGCGCCCGTGGAATTCTCGGGTGCCAAGGAACTCC303710.14914258116205106RNA PCR Primer, Index 1 (100% over 27bp)
CCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG284740.1398270012843911RNA PCR Primer, Index 1 (100% over 29bp)
GGGAGAGCGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGC260000.12767795298848664RNA PCR Primer, Index 3 (100% over 39bp)
GGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAACTCC258890.12713286634303578RNA PCR Primer, Index 1 (100% over 27bp)
AGTCGAGCTCAACCCGCAACGCGATGGAATTCTCGGGTGCCAAGGAACTC258590.12698554562804906RNA PCR Primer, Index 1 (100% over 26bp)
CGCCGCGACCGGCTCCGGGACGGCTGGGTGGAATTCTCGGGTGCCAAGGA255230.12533555362019788RNA PCR Primer, Index 1 (100% over 22bp)
ATGTACACGGAGTCGAGCTCAACCCGTGGAATTCTCGGGTGCCAAGGAAC250450.12298824356140954RNA PCR Primer, Index 1 (100% over 24bp)
ATGTACACGGAGTCGAGCTCAACCCTGGAATTCTCGGGTGCCAAGGAACT245380.12049852347813404RNA PCR Primer, Index 1 (100% over 25bp)
GAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAGGAAC241050.11837219449182577RNA PCR Primer, Index 1 (100% over 24bp)
GGGAGAGCCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGC237120.11644229312549981RNA PCR Primer, Index 3 (100% over 39bp)
GATTTATAGGTAGAGGCGACTGGAATTCTCGGGTGCCAAGGAACTCCAGT236470.1161230982430286RNA PCR Primer, Index 1 (100% over 30bp)
GGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACT228750.11233204517737046RNA PCR Primer, Index 1 (100% over 25bp)
GGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACTC225240.11060839281202589RNA PCR Primer, Index 1 (100% over 26bp)
GGAATAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGC224790.1103874117395458RNA PCR Primer, Index 3 (100% over 39bp)
ATGTACACGGAGTCGAGCTCAACTGGAATTCTCGGGTGCCAAGGAACTCC224420.1102057161910622RNA PCR Primer, Index 1 (100% over 27bp)
ATTGATCATCGACACTTCGAACGCACTGGAATTCTCGGGTGCCAAGGAAC224270.11013205583356885RNA PCR Primer, Index 1 (100% over 24bp)
GAATACAAGCTTGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT215030.10559457781197802RNA PCR Primer, Index 3 (100% over 34bp)
ATGTACACGGAGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTA211640.10392985373262813RNA PCR Primer, Index 3 (100% over 36bp)
ACATATACTAAAATTGGAACGATACAGAGAATGGAATTCTCGGGTGCCAA206360.10133700914886194No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)