FastQCFastQC Report
Tue 18 Jul 2023
SRR10530148_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10530148_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17014741
Total Bases850.7 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGA175974510.3424730355872RNA PCR Primer, Index 1 (100% over 22bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGC7253344.2629740881744835RNA PCR Primer, Index 11 (100% over 50bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG5468063.2137192097135068Illumina Small RNA Adapter 2 (100% over 21bp)
ATGTACACGGAGTCGACCCAACGCGATGGAATTCTCGGGTGCCAAGGAAC4399672.5857989845393474RNA PCR Primer, Index 1 (100% over 24bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGG4101012.4102688368867913Illumina Small RNA Adapter 2 (100% over 21bp)
AGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAACTC3543312.0824942325010998RNA PCR Primer, Index 1 (100% over 26bp)
AAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAACT3189801.8747273320234497RNA PCR Primer, Index 1 (100% over 25bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA2385831.4022135276699188RNA PCR Primer, Index 1 (100% over 23bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG2018701.1864418036101756No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC1951351.146858479949827RNA PCR Primer, Index 1 (100% over 24bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAG1425800.837979255752409No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGA1310350.7701263275180034RNA PCR Primer, Index 1 (100% over 22bp)
AAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAAC1274530.7490739941324996RNA PCR Primer, Index 1 (100% over 24bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA1153820.678129628890619No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAA1004510.5903763095776774RNA PCR Primer, Index 1 (100% over 23bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAA783620.46055358703373744No Hit
TTTTTTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGGAA770670.45294253964841424RNA PCR Primer, Index 1 (100% over 23bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAGG703330.4133650932447341Illumina Small RNA Adapter 2 (100% over 21bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAA689730.40537202417597773RNA PCR Primer, Index 1 (100% over 23bp)
TTTTTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGGAAC686800.40364998797219426RNA PCR Primer, Index 1 (100% over 24bp)
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGATGGAATTCTCGGGTGC670420.39402304154967743No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGTGGAATTCTCGGGTGCCAAGGA630830.37075498240026106RNA PCR Primer, Index 1 (100% over 22bp)
TAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACT626200.368033812562883RNA PCR Primer, Index 1 (100% over 25bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG574930.33790111762500524No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAG547910.3220207701075203No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA508560.2988937651181408No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA428040.25157009442576883RNA PCR Primer, Index 1 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG423490.24889594264173634No Hit
CTAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC397870.23383841105779984RNA PCR Primer, Index 1 (100% over 24bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGTGGAATTCTCGGGT388800.22850773925973955No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG384960.2262508726991495No Hit
ATGTACACGGAGTCGAGCTCAACCTGGAATTCTCGGGTGCCAAGGAACTC356470.20950656845143867RNA PCR Primer, Index 1 (100% over 26bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAA350350.2059096873704983No Hit
CGATCAAAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCA349370.2053337162170144No Hit
ACATATACTAAAATTGGAACGATACAGAGAATGGAATTCTCGGGTGCCAA344300.2023539470862354No Hit
ATTGATCATCGACACTTCGAACGCATGGAATTCTCGGGTGCCAAGGAACT331020.19454895023086158RNA PCR Primer, Index 1 (100% over 25bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGA323440.19009398967636357RNA PCR Primer, Index 1 (100% over 22bp)
AAAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAA323020.18984714489629903RNA PCR Primer, Index 1 (100% over 23bp)
ATGTACACGGAGTCGAGCTCAACCCGTGGAATTCTCGGGTGCCAAGGAAC321040.18868344807599483RNA PCR Primer, Index 1 (100% over 24bp)
AGGGCTTCTGTCGTGAGTGGCACACGTAGGTGGAATTCTCGGGTGCCAAG308900.18154845848079615No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA307800.18090196024729382No Hit
ATGTACACGGAGTCGACCAACGCGATGGAATTCTCGGGTGCCAAGGAACT304890.17919167855684667RNA PCR Primer, Index 1 (100% over 25bp)
CCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCA303330.17827482651660698RNA PCR Primer, Index 1 (100% over 28bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA276160.16230632014909896No Hit
GGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACT269720.1585213668547761RNA PCR Primer, Index 1 (100% over 25bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTTGGAATTCTCGGGTGCC269040.1581217134013383No Hit
AAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTC268540.1578278505679281RNA PCR Primer, Index 1 (100% over 26bp)
ATGTACACGGAGTCGACCCAACGCGACTCCCGGGGCTACGCCTGTCTGAG261100.15345517160678496No Hit
GGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACTC249580.1466845719250149RNA PCR Primer, Index 1 (100% over 26bp)
ATGTACACGGAGTCGCCCAACGCGATGGAATTCTCGGGTGCCAAGGAACT249270.14650237696830062RNA PCR Primer, Index 1 (100% over 25bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCA248930.1463025502415817No Hit
ACGATCAAAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCC239720.14088959685016655No Hit
GGAATAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTAC224100.13170932193443322RNA PCR Primer, Index 11 (100% over 39bp)
AACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTCC221830.1303751846707511RNA PCR Primer, Index 1 (100% over 27bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGG221410.13012833989068656Illumina Small RNA Adapter 2 (100% over 21bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGTGGAATTCTCGGGTGCCAAG215300.1265373360664144No Hit
ATGTACACGGAGTCGAGCTCAACCCTGGAATTCTCGGGTGCCAAGGAACT212260.12475065003928064RNA PCR Primer, Index 1 (100% over 25bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCT202730.11914962443448301RNA PCR Primer, Index 11 (100% over 43bp)
GGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAACTCC202000.11872058469770419RNA PCR Primer, Index 1 (100% over 27bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGGAATTCTCGGGTGCCA199350.11716311168063033No Hit
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCG197450.11604643291367174RNA PCR Primer, Index 11 (100% over 45bp)
ATTGATCATCGACACTTCGAACGCACTGGAATTCTCGGGTGCCAAGGAAC192220.11297262767620148RNA PCR Primer, Index 1 (100% over 24bp)
ATGTACACGGAGTCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA191620.11261999227610928RNA PCR Primer, Index 1 (100% over 32bp)
GGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAAC190470.11194410775926592RNA PCR Primer, Index 1 (100% over 24bp)
ATGTACACGGAGTCGAGCTCAACCCGCTGGAATTCTCGGGTGCCAAGGAA189680.11147980448247787RNA PCR Primer, Index 1 (100% over 23bp)
TTAACCAGTGAAATTGACCTGCCCGTGATGGAATTCTCGGGTGCCAAGGA178800.10508534922747281RNA PCR Primer, Index 1 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)