FastQCFastQC Report
Tue 18 Jul 2023
SRR10530149_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10530149_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18117560
Total Bases905.8 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGA191784310.585547943542066RNA PCR Primer, Index 1 (100% over 22bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGC7452574.113451259441117RNA PCR Primer, Index 11 (100% over 50bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG5991773.30716167077686Illumina Small RNA Adapter 2 (100% over 21bp)
ATGTACACGGAGTCGACCCAACGCGATGGAATTCTCGGGTGCCAAGGAAC4741592.617123939426722RNA PCR Primer, Index 1 (100% over 24bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGG4494142.4805437376777006Illumina Small RNA Adapter 2 (100% over 21bp)
AGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAACTC3837302.118000437144958RNA PCR Primer, Index 1 (100% over 26bp)
AAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAACT3480071.9208270870911976RNA PCR Primer, Index 1 (100% over 25bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA2467101.3617175822792915RNA PCR Primer, Index 1 (100% over 23bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG2202411.2156217503902291No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC2093561.1555419162403766RNA PCR Primer, Index 1 (100% over 24bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAG1570710.8669544905605391No Hit
AAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAAC1404770.7753637907091242RNA PCR Primer, Index 1 (100% over 24bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGA1326030.7319031922620927RNA PCR Primer, Index 1 (100% over 22bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA1248320.6890111030403653No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAA1106920.6109652734694959RNA PCR Primer, Index 1 (100% over 23bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAA853040.47083602869260543No Hit
TTTTTTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGGAA851850.47017920735463276RNA PCR Primer, Index 1 (100% over 23bp)
TTTTTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGGAAC735570.40599837947273254RNA PCR Primer, Index 1 (100% over 24bp)
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGATGGAATTCTCGGGTGC733680.40495519264183477No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAA727160.4013564740505896RNA PCR Primer, Index 1 (100% over 23bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAGG719960.3973824289805029Illumina Small RNA Adapter 2 (100% over 21bp)
TAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACT663970.366478709053537RNA PCR Primer, Index 1 (100% over 25bp)
CGCCGCGACCGGCTCCGGGACGGCTGGGTGGAATTCTCGGGTGCCAAGGA622250.3434513256752013RNA PCR Primer, Index 1 (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG584400.3225599915220372No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAG573100.3163229485648178No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA554570.3060953020163863No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG461590.25477492554185No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA449740.24823430969733232RNA PCR Primer, Index 1 (100% over 22bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGTGGAATTCTCGGGT417040.23018552167068854No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG400830.2212384007559517No Hit
CTAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC398190.21978125089691988RNA PCR Primer, Index 1 (100% over 24bp)
CGATCAAAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCA388400.21437765350301036No Hit
ATGTACACGGAGTCGAGCTCAACCTGGAATTCTCGGGTGCCAAGGAACTC382480.21111010533427238RNA PCR Primer, Index 1 (100% over 26bp)
ACATATACTAAAATTGGAACGATACAGAGAATGGAATTCTCGGGTGCCAA368990.20366429033490163No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAA361470.19951362103947773No Hit
AAAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAA354420.19562236857501783RNA PCR Primer, Index 1 (100% over 23bp)
ATTGATCATCGACACTTCGAACGCATGGAATTCTCGGGTGCCAAGGAACT349080.1926749518147035RNA PCR Primer, Index 1 (100% over 25bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGA347520.19181390871618473RNA PCR Primer, Index 1 (100% over 22bp)
ATGTACACGGAGTCGAGCTCAACCCGTGGAATTCTCGGGTGCCAAGGAAC347420.19175871364576685RNA PCR Primer, Index 1 (100% over 24bp)
AGGGCTTCTGTCGTGAGTGGCACACGTAGGTGGAATTCTCGGGTGCCAAG335200.18501387604070307No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA325890.17987521498479928No Hit
ATGTACACGGAGTCGACCAACGCGATGGAATTCTCGGGTGCCAAGGAACT324300.17899761336515513RNA PCR Primer, Index 1 (100% over 25bp)
CCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCA321460.1774300733652876RNA PCR Primer, Index 1 (100% over 28bp)
ATGTACACGGAGTCGACCCAACGCGACTCCCGGGGCTACGCCTGTCTGAG299150.16511605315506062No Hit
GGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACT295130.16289721132426221RNA PCR Primer, Index 1 (100% over 25bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTTGGAATTCTCGGGTGCC289870.15999395062028218No Hit
AAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTC288600.15929297322597524RNA PCR Primer, Index 1 (100% over 26bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA283710.15659393428254137No Hit
ACGATCAAAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCC270540.14932474350850777No Hit
ATGTACACGGAGTCGCCCAACGCGATGGAATTCTCGGGTGCCAAGGAACT269970.1490101316071259RNA PCR Primer, Index 1 (100% over 25bp)
GGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACTC269180.14857409055082474RNA PCR Primer, Index 1 (100% over 26bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCA258200.1425136718189425No Hit
AACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTCC239250.13205420597475598RNA PCR Primer, Index 1 (100% over 27bp)
GGAATAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTAC236750.13067432921430921RNA PCR Primer, Index 11 (100% over 39bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGG232340.12824022660888112Illumina Small RNA Adapter 2 (100% over 21bp)
ATGTACACGGAGTCGAGCTCAACCCTGGAATTCTCGGGTGCCAAGGAACT230860.12742333956669663RNA PCR Primer, Index 1 (100% over 25bp)
GGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAACTCC226690.1251217051302714RNA PCR Primer, Index 1 (100% over 27bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGTGGAATTCTCGGGTGCCAAG224760.1240564402712065No Hit
ATTGATCATCGACACTTCGAACGCACTGGAATTCTCGGGTGCCAAGGAAC213980.11810641168016002RNA PCR Primer, Index 1 (100% over 24bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCT211790.11689763963800864RNA PCR Primer, Index 11 (100% over 43bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGGAATTCTCGGGTGCCA211190.11656646921550144No Hit
GGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAAC208140.11488301956775636RNA PCR Primer, Index 1 (100% over 24bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCG207710.11464568076495951RNA PCR Primer, Index 11 (100% over 45bp)
ATGTACACGGAGTCGAGCTCAACCCGCTGGAATTCTCGGGTGCCAAGGAA207250.11439178344103731RNA PCR Primer, Index 1 (100% over 23bp)
ATGTACACGGAGTCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA206750.11411580808894796RNA PCR Primer, Index 1 (100% over 32bp)
TTAACCAGTGAAATTGACCTGCCCGTGATGGAATTCTCGGGTGCCAAGGA193970.10706187808954407RNA PCR Primer, Index 1 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)