FastQCFastQC Report
Tue 18 Jul 2023
SRR10530150_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10530150_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30024687
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATGC627679520.905446907739623RNA PCR Primer, Index 12 (100% over 50bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGA5284201.7599517357166787RNA PCR Primer, Index 1 (100% over 22bp)
CGCCGCGACCGGCTCCGGGACGGCTGGGTGGAATTCTCGGGTGCCAAGGA2938740.9787745664093017RNA PCR Primer, Index 1 (100% over 22bp)
CTAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC2028190.6755074582459428RNA PCR Primer, Index 1 (100% over 24bp)
CGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG1807900.6021378341096445RNA PCR Primer, Index 12 (100% over 45bp)
AGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAACTC1786250.594927101155126RNA PCR Primer, Index 1 (100% over 26bp)
AAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAACT1669560.556062416237678RNA PCR Primer, Index 1 (100% over 25bp)
TAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACT1608730.535802421520664RNA PCR Primer, Index 1 (100% over 25bp)
GCAGGACACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAA1457250.48535060498715604RNA PCR Primer, Index 12 (100% over 40bp)
CCCCTTGTAAATTTAACTGTTAGTCCATGGAATTCTCGGGTGCCAAGGAA1394290.4643811940487506RNA PCR Primer, Index 1 (100% over 23bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG1127830.375634223930461RNA PCR Primer, Index 12 (100% over 45bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTG1091620.3635741481668069RNA PCR Primer, Index 12 (100% over 37bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG1083860.3609896083179818Illumina Small RNA Adapter 2 (100% over 21bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGG941410.3135453168920629Illumina Small RNA Adapter 2 (100% over 21bp)
CCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCA914980.30474256068014965RNA PCR Primer, Index 1 (100% over 28bp)
GGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGT879150.29280904743486585RNA PCR Primer, Index 12 (100% over 46bp)
ACATATACTAAAATTGGAACGATACAGAGAATGGAATTCTCGGGTGCCAA865770.2883527145511958No Hit
AAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAAC828490.27593626538055166RNA PCR Primer, Index 1 (100% over 24bp)
AACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTCC775070.2581442397717585RNA PCR Primer, Index 1 (100% over 27bp)
CGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG702800.2340740471332807RNA PCR Primer, Index 12 (100% over 45bp)
CGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC701690.23370435135593587RNA PCR Primer, Index 12 (100% over 44bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGTGGAATTCTCGGGTGCCAAG700310.23324472957869635No Hit
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC697920.23244871794999897RNA PCR Primer, Index 12 (100% over 44bp)
ACGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC657910.21912301700264183RNA PCR Primer, Index 12 (100% over 44bp)
CCCTTGTAAATTTAACTGTTAGTCCATGGAATTCTCGGGTGCCAAGGAAC647780.21574912671029675RNA PCR Primer, Index 1 (100% over 24bp)
AAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTC634930.21146931523382742RNA PCR Primer, Index 1 (100% over 26bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAG634500.21132609975251368No Hit
GAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC629880.2097873659765379RNA PCR Primer, Index 12 (100% over 44bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA628610.209364380717774No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAGG628140.20920784286610547Illumina Small RNA Adapter 2 (100% over 21bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC624240.20790891175651557RNA PCR Primer, Index 2 (100% over 34bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA613240.20424525990895426RNA PCR Primer, Index 1 (100% over 22bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCT541140.18023168734448422RNA PCR Primer, Index 12 (100% over 43bp)
ATGTACACGGAGTCGACCCAACGCGATGGAATTCTCGGGTGCCAAGGAAC497640.1657436095836736RNA PCR Primer, Index 1 (100% over 24bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTTGGAATTCTCGGGTGCC487930.16250960418005356No Hit
CCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC481070.1602248176642108RNA PCR Primer, Index 12 (100% over 44bp)
CCCCTTGTAAATTTAACTGTTAGTCCAATGGAATTCTCGGGTGCCAAGGA474200.1579367005557793RNA PCR Primer, Index 1 (100% over 22bp)
TTAACCAGTGAAATTGACCTGCCCGTGATGGAATTCTCGGGTGCCAAGGA471500.15703744055683244RNA PCR Primer, Index 1 (100% over 22bp)
CGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC465890.15516897811457617RNA PCR Primer, Index 12 (100% over 44bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAG457660.15242790041408258No Hit
CGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG450020.14988332767632181RNA PCR Primer, Index 12 (100% over 45bp)
AGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG446000.1485444294556676RNA PCR Primer, Index 12 (100% over 45bp)
ATTGATCATCGACACTTCGAACGCATGGAATTCTCGGGTGCCAAGGAACT418440.13936531628123217RNA PCR Primer, Index 1 (100% over 25bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCCA403370.13434611325007317No Hit
CGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCT401520.13372995362116513RNA PCR Primer, Index 12 (100% over 43bp)
GCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG401350.13367333354715738RNA PCR Primer, Index 12 (100% over 45bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG400450.13337358021417509No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAA391580.1304193445880052No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG388930.1295367375520018No Hit
CGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGT374410.12470071711322087RNA PCR Primer, Index 12 (100% over 46bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGGAATTCTCGGGTGCCA372170.12395466437335383No Hit
CCCCTTGTAAATTTAACTGTTAGTCCTGGAATTCTCGGGTGCCAAGGAAC371490.12372818407732278RNA PCR Primer, Index 1 (100% over 24bp)
AGGTCGGTTTCTATCTACTTCAAATTCCTTGGAATTCTCGGGTGCCAAGG362710.12080392378445112Illumina Small RNA Adapter 2 (100% over 21bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTATCTCGTATGCC351000.11690379986309266RNA PCR Primer, Index 12 (97% over 39bp)
GGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG332780.11083546016649566RNA PCR Primer, Index 12 (100% over 45bp)
AAAGCTGAACAAAATGAGTGAAAACTTGGAATTCTCGGGTGCCAAGGAAC323210.10764808305911731RNA PCR Primer, Index 1 (100% over 24bp)
GCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCC321090.1069419974303146RNA PCR Primer, Index 1 (100% over 27bp)
CACAAAGCACCCAACTTACACTTAGGAGATTTGGAATTCTCGGGTGCCAA320860.10686539380077467No Hit
CCCCTTGTAAATTTAACTGTTAGTCCAAAGTGGAATTCTCGGGTGCCAAG313570.10443739180361813No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAA310900.10354812358243734No Hit
GCGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCT309950.10323171728651159RNA PCR Primer, Index 12 (100% over 43bp)
GCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC307920.10255560699100709RNA PCR Primer, Index 12 (100% over 44bp)
AGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC303430.10106017091868434RNA PCR Primer, Index 12 (100% over 44bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA301350.1003674076602364No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)