FastQCFastQC Report
Tue 18 Jul 2023
SRR10530151_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10530151_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31253853
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATGC644176720.611113132195253RNA PCR Primer, Index 12 (100% over 50bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGA5689451.8203995520168346RNA PCR Primer, Index 1 (100% over 22bp)
CGCCGCGACCGGCTCCGGGACGGCTGGGTGGAATTCTCGGGTGCCAAGGA2865010.9166901757680885RNA PCR Primer, Index 1 (100% over 22bp)
CTAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC2000070.6399434975265289RNA PCR Primer, Index 1 (100% over 24bp)
AGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAACTC1907950.6104687316472629RNA PCR Primer, Index 1 (100% over 26bp)
CGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG1885890.603410401911086RNA PCR Primer, Index 12 (100% over 45bp)
AAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAACT1803610.5770840478452369RNA PCR Primer, Index 1 (100% over 25bp)
TAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACT1712800.5480284302866594RNA PCR Primer, Index 1 (100% over 25bp)
GCAGGACACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAA1520650.48654801057648794RNA PCR Primer, Index 12 (100% over 40bp)
CCCCTTGTAAATTTAACTGTTAGTCCATGGAATTCTCGGGTGCCAAGGAA1482970.4744918970470617RNA PCR Primer, Index 1 (100% over 23bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG1176390.37639839158391125RNA PCR Primer, Index 12 (100% over 45bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG1153900.3692024788111725Illumina Small RNA Adapter 2 (100% over 21bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTG1067370.3415162924072114RNA PCR Primer, Index 12 (100% over 37bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGG995660.31857192135638446Illumina Small RNA Adapter 2 (100% over 21bp)
CCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCA956520.3060486654237479RNA PCR Primer, Index 1 (100% over 28bp)
ACATATACTAAAATTGGAACGATACAGAGAATGGAATTCTCGGGTGCCAA931170.29793766547759726No Hit
GGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGT918880.2940053503163274RNA PCR Primer, Index 12 (100% over 46bp)
AAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAAC881700.2821092170619731RNA PCR Primer, Index 1 (100% over 24bp)
AACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTCC817500.26156774974272773RNA PCR Primer, Index 1 (100% over 27bp)
CGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC740330.2368763940881145RNA PCR Primer, Index 12 (100% over 44bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGTGGAATTCTCGGGTGCCAAG727260.23269450969773234No Hit
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC725730.23220497005601196RNA PCR Primer, Index 12 (100% over 44bp)
CGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG720960.23067875823182504RNA PCR Primer, Index 12 (100% over 45bp)
CCCTTGTAAATTTAACTGTTAGTCCATGGAATTCTCGGGTGCCAAGGAAC699860.22392759062378645RNA PCR Primer, Index 1 (100% over 24bp)
ACGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC685600.2193649531787329RNA PCR Primer, Index 12 (100% over 44bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAG677120.21665168771351165No Hit
AAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTC676990.21661009284199298RNA PCR Primer, Index 1 (100% over 26bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA673580.21551902736600187No Hit
GAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC660280.21126355204908656RNA PCR Primer, Index 12 (100% over 44bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAGG635180.2032325422404719Illumina Small RNA Adapter 2 (100% over 21bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA631380.2020166921499247RNA PCR Primer, Index 1 (100% over 22bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC610210.195243127303376RNA PCR Primer, Index 2 (100% over 34bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCT561220.179568259951821RNA PCR Primer, Index 12 (100% over 43bp)
ATGTACACGGAGTCGACCCAACGCGATGGAATTCTCGGGTGCCAAGGAAC530190.16963988408085237RNA PCR Primer, Index 1 (100% over 24bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTTGGAATTCTCGGGTGCC528790.16919193931065074No Hit
CCCCTTGTAAATTTAACTGTTAGTCCAATGGAATTCTCGGGTGCCAAGGA508630.16274153461974752RNA PCR Primer, Index 1 (100% over 22bp)
TTAACCAGTGAAATTGACCTGCCCGTGATGGAATTCTCGGGTGCCAAGGA505420.16171446125378525RNA PCR Primer, Index 1 (100% over 22bp)
CCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC498090.15936915042122968RNA PCR Primer, Index 12 (100% over 44bp)
CGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC485810.1554400348654612RNA PCR Primer, Index 12 (100% over 44bp)
CGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG469310.15016068578808509RNA PCR Primer, Index 12 (100% over 45bp)
AGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG466730.14933518756871353RNA PCR Primer, Index 12 (100% over 45bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAG456030.14591160968217262No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAA453250.14502211935277226No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCCA448200.14340631857454503No Hit
ATTGATCATCGACACTTCGAACGCATGGAATTCTCGGGTGCCAAGGAACT439540.1406354602102979RNA PCR Primer, Index 1 (100% over 25bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG426330.13640878134289555No Hit
GCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG421840.13497215847274893RNA PCR Primer, Index 12 (100% over 45bp)
CGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCT415370.1329020137133172RNA PCR Primer, Index 12 (100% over 43bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGGAATTCTCGGGTGCCA397090.1270531348566847No Hit
AGGTCGGTTTCTATCTACTTCAAATTCCTTGGAATTCTCGGGTGCCAAGG393280.12583408516063602Illumina Small RNA Adapter 2 (100% over 21bp)
CCCCTTGTAAATTTAACTGTTAGTCCTGGAATTCTCGGGTGCCAAGGAAC391390.12522935972086385RNA PCR Primer, Index 1 (100% over 24bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG389070.12448705124452975No Hit
CGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGT387230.12389832383226478RNA PCR Primer, Index 12 (100% over 46bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAAGCTCGTATGC368950.11804944497563229RNA PCR Primer, Index 12 (98% over 50bp)
GGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG351490.11246293377011787RNA PCR Primer, Index 12 (100% over 45bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTATCTCGTATGCC350030.11199579136690764RNA PCR Primer, Index 12 (97% over 39bp)
AAAGCTGAACAAAATGAGTGAAAACTTGGAATTCTCGGGTGCCAAGGAAC345570.11056876731326536RNA PCR Primer, Index 1 (100% over 24bp)
CACAAAGCACCCAACTTACACTTAGGAGATTTGGAATTCTCGGGTGCCAA345510.1105495696802567No Hit
GCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCC337610.10802188133411904RNA PCR Primer, Index 1 (100% over 27bp)
CCCCTTGTAAATTTAACTGTTAGTCCAAAGTGGAATTCTCGGGTGCCAAG331580.10609251921675067No Hit
GCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC328680.10516463362133302RNA PCR Primer, Index 12 (100% over 44bp)
GCGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCT326790.10455990818156084RNA PCR Primer, Index 12 (100% over 43bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAA325520.10415355828287795No Hit
AGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC318950.10205141746843181RNA PCR Primer, Index 12 (100% over 44bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA314260.10055080248825642No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)