FastQCFastQC Report
Tue 18 Jul 2023
SRR10530152_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10530152_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38271716
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGTATGC908547823.739405883969248RNA PCR Primer, Index 13 (100% over 50bp)
CGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCG3667710.958334348007808RNA PCR Primer, Index 13 (100% over 45bp)
CGCCGCGACCGGCTCCGGGACGGCTGGGTGGAATTCTCGGGTGCCAAGGA3466930.9058726292805894RNA PCR Primer, Index 1 (100% over 22bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTC2795780.7305081381770286RNA PCR Primer, Index 13 (100% over 37bp)
ATGTACACGGAGTCGACCCAACGCGATGGAATTCTCGGGTGCCAAGGAAC2540570.6638244284630457RNA PCR Primer, Index 1 (100% over 24bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCG2007990.5246668322894119RNA PCR Primer, Index 13 (100% over 45bp)
CGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCG1933330.5051589534161468RNA PCR Primer, Index 13 (100% over 45bp)
ACGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTC1794560.4688998005733529RNA PCR Primer, Index 13 (100% over 44bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTC1501110.39222437791919235RNA PCR Primer, Index 13 (100% over 44bp)
GGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGT1460840.38170224716341433RNA PCR Primer, Index 13 (100% over 46bp)
GCAGGACACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAA1435360.37504458906415383RNA PCR Primer, Index 13 (100% over 40bp)
CGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTC1432900.37440181673588924RNA PCR Primer, Index 13 (100% over 44bp)
CCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTC1387940.36265423792337925RNA PCR Primer, Index 13 (100% over 44bp)
CCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTC1221590.3191887188962209RNA PCR Primer, Index 13 (100% over 44bp)
CTAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC1172110.30626011125291586RNA PCR Primer, Index 1 (100% over 24bp)
TAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACT1134280.29637552703411574RNA PCR Primer, Index 1 (100% over 25bp)
AGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCG1083010.2829792110706507RNA PCR Primer, Index 13 (100% over 45bp)
CGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCG1058710.27662987465730565RNA PCR Primer, Index 13 (100% over 45bp)
CCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCG998220.26082446891067024RNA PCR Primer, Index 13 (100% over 45bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAATCTCGTATGCC982510.25671960985496445RNA PCR Primer, Index 13 (97% over 39bp)
AGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAACTC970250.25351619979621504RNA PCR Primer, Index 1 (100% over 26bp)
GCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCG935730.2444964840353644RNA PCR Primer, Index 13 (100% over 45bp)
GAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTC889660.2324588738064423RNA PCR Primer, Index 13 (100% over 44bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGA872690.2280247898996742RNA PCR Primer, Index 1 (100% over 22bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGTGGAATTCTCGGGTGCCAAG847550.22145597025228764No Hit
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCT831670.21730669197064487RNA PCR Primer, Index 13 (100% over 43bp)
ACGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCT816280.21328544557552634RNA PCR Primer, Index 13 (100% over 43bp)
CGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGT728840.19043828607005758RNA PCR Primer, Index 13 (100% over 46bp)
AAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAACT725520.18957080471646476RNA PCR Primer, Index 1 (100% over 25bp)
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGATGGAATTCTCGGGTGC720450.18824606662528537No Hit
CCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTC680890.17790945146018536RNA PCR Primer, Index 13 (100% over 44bp)
CGCCGCGACCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCA680320.17776051640851434RNA PCR Primer, Index 13 (100% over 38bp)
CGGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTC668810.1747530735230163RNA PCR Primer, Index 13 (100% over 44bp)
GCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTC663130.17326894879759247RNA PCR Primer, Index 13 (100% over 44bp)
ATTGATCATCGACACTTCGAACGCATGGAATTCTCGGGTGCCAAGGAACT584460.1527132987713433RNA PCR Primer, Index 1 (100% over 25bp)
GCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTC564910.14760508778859038RNA PCR Primer, Index 13 (100% over 44bp)
CGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTC563960.14735686270247197RNA PCR Primer, Index 13 (100% over 44bp)
CGCGACCTCAGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGT555580.1451672561533431RNA PCR Primer, Index 13 (100% over 36bp)
ATGTACACGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAA552230.14429193611282023RNA PCR Primer, Index 13 (100% over 39bp)
ACCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTC532180.13905308034789973RNA PCR Primer, Index 13 (100% over 44bp)
CCCCTTGTAAATTTAACTGTTAGTCCATGGAATTCTCGGGTGCCAAGGAA524470.13703853780687544RNA PCR Primer, Index 1 (100% over 23bp)
AACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTCC502960.1314181992780256RNA PCR Primer, Index 1 (100% over 27bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA499160.13042529893355187RNA PCR Primer, Index 1 (100% over 34bp)
CGGCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTC483290.12627863354755245RNA PCR Primer, Index 13 (100% over 44bp)
CGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCT467800.12223125819600042RNA PCR Primer, Index 13 (100% over 43bp)
GCGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCT460120.12022455434190617RNA PCR Primer, Index 13 (100% over 43bp)
GGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCG450660.11775275506329531RNA PCR Primer, Index 13 (100% over 45bp)
AAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTC449860.11754372341182716RNA PCR Primer, Index 1 (100% over 26bp)
GGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTC444400.11611708239055703RNA PCR Primer, Index 13 (100% over 44bp)
CGGCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTC443080.11577218016563459RNA PCR Primer, Index 13 (100% over 44bp)
AGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTC440800.11517643995895037RNA PCR Primer, Index 13 (100% over 44bp)
ATGTACACGGAGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGT436740.1141156043277495RNA PCR Primer, Index 13 (100% over 36bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAGG419820.10969458489919816Illumina Small RNA Adapter 2 (100% over 21bp)
AAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAAC416760.10889503883233247RNA PCR Primer, Index 1 (100% over 24bp)
GGGAGAGATCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAA414310.10825487939971126RNA PCR Primer, Index 13 (100% over 39bp)
GAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCG412340.10774013895797094RNA PCR Primer, Index 13 (100% over 45bp)
NGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGTATGC397840.10395144027511073RNA PCR Primer, Index 13 (98% over 50bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAG391160.1022060259853517No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)