FastQCFastQC Report
Tue 18 Jul 2023
SRR10530153_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10530153_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39697317
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGTATGC935768823.5725955988411RNA PCR Primer, Index 13 (100% over 50bp)
CGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCG3828430.9644052266806847RNA PCR Primer, Index 13 (100% over 45bp)
CGCCGCGACCGGCTCCGGGACGGCTGGGTGGAATTCTCGGGTGCCAAGGA3327050.8381044996063588RNA PCR Primer, Index 1 (100% over 22bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTC2792670.7034908681611909RNA PCR Primer, Index 13 (100% over 37bp)
ATGTACACGGAGTCGACCCAACGCGATGGAATTCTCGGGTGCCAAGGAAC2709100.6824390676075162RNA PCR Primer, Index 1 (100% over 24bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCG2097460.5283631636868558RNA PCR Primer, Index 13 (100% over 45bp)
CGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCG2021270.509170430837933RNA PCR Primer, Index 13 (100% over 45bp)
ACGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTC1870930.4712988537739213RNA PCR Primer, Index 13 (100% over 44bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTC1562900.3937041891269377RNA PCR Primer, Index 13 (100% over 44bp)
GCAGGACACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAA1509120.3801566740643958RNA PCR Primer, Index 13 (100% over 40bp)
GGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGT1508040.3798846153758956RNA PCR Primer, Index 13 (100% over 46bp)
CGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTC1494270.37641586709751695RNA PCR Primer, Index 13 (100% over 44bp)
CCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTC1454170.36631442875597864RNA PCR Primer, Index 13 (100% over 44bp)
CCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTC1273780.3208730705906397RNA PCR Primer, Index 13 (100% over 44bp)
TAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACT1198880.30200529673075893RNA PCR Primer, Index 1 (100% over 25bp)
CTAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC1176350.29632985020121133RNA PCR Primer, Index 1 (100% over 24bp)
AGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCG1119280.28195356376351577RNA PCR Primer, Index 13 (100% over 45bp)
CGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCG1102770.2777945925161643RNA PCR Primer, Index 13 (100% over 45bp)
CCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCG1043450.26285151714409316RNA PCR Primer, Index 13 (100% over 45bp)
AGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAACTC1025450.2583172056690884RNA PCR Primer, Index 1 (100% over 26bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAATCTCGTATGCC991800.24984056227276016RNA PCR Primer, Index 13 (97% over 39bp)
GCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCG990560.24952819859337094RNA PCR Primer, Index 13 (100% over 45bp)
GAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTC931030.2345322229207581RNA PCR Primer, Index 13 (100% over 44bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGA891010.22445093707466426RNA PCR Primer, Index 1 (100% over 22bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGTGGAATTCTCGGGTGCCAAG883640.2225943884318429No Hit
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCT859430.21649573949796153RNA PCR Primer, Index 13 (100% over 43bp)
ACGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCT850440.21423110282238972RNA PCR Primer, Index 13 (100% over 43bp)
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGATGGAATTCTCGGGTGC777800.19593263695881513No Hit
AAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAACT774310.19505348434505032RNA PCR Primer, Index 1 (100% over 25bp)
CGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGT749380.1887734629521688RNA PCR Primer, Index 13 (100% over 46bp)
CGCCGCGACCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCA714890.18008521835367364RNA PCR Primer, Index 13 (100% over 38bp)
CCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTC708300.1784251565414358RNA PCR Primer, Index 13 (100% over 44bp)
GCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTC701090.17660891288950334RNA PCR Primer, Index 13 (100% over 44bp)
CGGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTC694930.1750571707402795RNA PCR Primer, Index 13 (100% over 44bp)
ATTGATCATCGACACTTCGAACGCATGGAATTCTCGGGTGCCAAGGAACT629220.15850441479458172RNA PCR Primer, Index 1 (100% over 25bp)
GCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTC593800.14958189743654465RNA PCR Primer, Index 13 (100% over 44bp)
ATGTACACGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAA587050.14788153063341788RNA PCR Primer, Index 13 (100% over 39bp)
CGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTC586430.14772534879372326RNA PCR Primer, Index 13 (100% over 44bp)
CGCGACCTCAGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGT585860.14758176226368144RNA PCR Primer, Index 13 (100% over 36bp)
ACCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTC563860.14203982601645346RNA PCR Primer, Index 13 (100% over 44bp)
CCCCTTGTAAATTTAACTGTTAGTCCATGGAATTCTCGGGTGCCAAGGAA557780.1405082363626741RNA PCR Primer, Index 1 (100% over 23bp)
AACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTCC535910.13499904792054335RNA PCR Primer, Index 1 (100% over 27bp)
CGGCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTC504540.12709675064438233RNA PCR Primer, Index 13 (100% over 44bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA489450.12329548619117005RNA PCR Primer, Index 1 (100% over 34bp)
CGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCT484050.12193519274866862RNA PCR Primer, Index 13 (100% over 43bp)
AAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTC479680.12083436268501471RNA PCR Primer, Index 1 (100% over 26bp)
GCGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCT478760.12060260898740335RNA PCR Primer, Index 13 (100% over 43bp)
GGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTC474340.11948918361409663RNA PCR Primer, Index 13 (100% over 44bp)
GGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCG470810.1185999547526096RNA PCR Primer, Index 13 (100% over 45bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAAGCTCGTATGC467950.11787950304046997RNA PCR Primer, Index 13 (98% over 50bp)
CGGCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTC462760.11657210989851026RNA PCR Primer, Index 13 (100% over 44bp)
AGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTC461970.11637310400599618RNA PCR Primer, Index 13 (100% over 44bp)
ATGTACACGGAGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGT461560.1162698224668433RNA PCR Primer, Index 13 (100% over 36bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAA453070.1141311388877994No Hit
AAAGGGACAAGCATCAAGCACGCAGCTGGAATTCTCGGGTGCCAAGGAAC449080.11312603317750669RNA PCR Primer, Index 1 (100% over 24bp)
GGGAGAGATCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAA431140.10860683607408532RNA PCR Primer, Index 13 (100% over 39bp)
GAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCG428610.1079695134056541RNA PCR Primer, Index 13 (100% over 45bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAGG424020.1068132639795279Illumina Small RNA Adapter 2 (100% over 21bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAG404540.10190613133880055No Hit
CCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCT402170.10130911366125826RNA PCR Primer, Index 13 (100% over 43bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)