FastQCFastQC Report
Tue 18 Jul 2023
SRR10533462_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10533462_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42924997
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT9474182.207147504285207No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT7236011.685733373493305Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC6567411.5299733160144426No Hit
GCTTAACACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGC6063611.4126058063556766No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC5739221.3370344557042135No Hit
CTTAACACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCA5487611.2784182605766985No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGT4247410.9894957010713362No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC4237900.9872802087790478No Hit
TTAACACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCAC4069690.9480932520507807No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT3717120.8659569620936722Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG3644080.8489412358025324No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT3611030.8412417594344853No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG3465630.8073687227048612TruSeq Adapter, Index 4 (100% over 49bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC3342540.7786931237292807No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACG2144550.499603995313034No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCAC2124280.4948818051169579No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG2081190.48484336527734645No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC2070420.48233433772866663No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT2055590.47887947435383627Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTG1791380.4173279266624061Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGCGGGGCGCGGGACATGTGGCGTAGATCGGAAGAGCACACGTCTGAAC1592140.3709120818342748Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC1581560.3684473175385429No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC1543320.3595387554715496No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA1342530.31276181568515893No Hit
AAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTCTG1233840.2874409053540528Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGAGAGCGACGCTCAGACAGGCGTAGCCCCGGGAGGAGATCGGAAGAGC1215760.28322890738932377No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACGT1209790.28183810938880205No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA1189180.277036711266398No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGAAGAGATCGGAAGAGCACACGT1188790.27694585511561015No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT1180170.2749377012187095No Hit
AGCTGGGGCGATCCACGGGAAGGGCCCGGCTCGCGTCCAGATCGGAAGAG1138490.2652277413088695No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC1071820.2496959988139312No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACAC984610.22937916571083278No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA980290.22837275911749044No Hit
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCA978750.22801399380412302No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACA971430.22630869374318185No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA957860.22314736562474308No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACG932030.21712989286871703No Hit
AAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTCTGA929220.2164752626540661Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTCTGAAC909750.2119394440493496Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGAGAGCGACGCTCAGACAGGCGTAGCCCCGGGAGAGATCGGAAGAGCA829940.19334654816632835No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACGTC820850.19122890095950384No Hit
TAACACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACA794970.18519977997901782No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG777310.18108562709975262Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCAC773120.18010950589000624No Hit
ACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTC761030.17729296521558288No Hit
CCCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCAGATCGGAAGAG758630.17673385044150383No Hit
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCAGATCGGAAGAGCA753780.1756039726688857No Hit
ACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTCTGAACTC731750.17047176497181818Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGC713760.16628073381111708No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGCCGGAGATCGGAAGAGCAC666770.1553337324636272No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACGT665880.15512639406823955No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT665560.15505184543169565Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC651260.15172045323614117Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA606420.14127432554043043Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACG589120.1372440398772771No Hit
CACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGT582340.1356645406405037No Hit
CGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCA541990.12626442350129927No Hit
CCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTCTGAACTCCA527390.12286314195898487Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCTTAACACAAAGCACCCAACTTACAGATCGGAAGAGCACACGTCTGAAC518540.12080140622956828Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT512630.11942458609839855Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC507970.11833897157872836No Hit
GAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGAGATCGGAAGAGC493370.11493769003641396No Hit
GGGAGAGCGACGCTCAGACAGGCGTAGCCCCGGGAGGAAAGATCGGAAGA453530.10565638478670134No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGTCCGGCCGTGAGATCGGAA452880.10550495786872158No Hit
GGGAGAGCGACGCTCAGACAGGCGTAGCCCCGGGAGGAAGATCGGAAGAG449840.10479674582155474No Hit
GCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAACT435460.10144671646686429Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACGTCT431060.10042167271438597Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)