FastQCFastQC Report
Tue 25 Jul 2023
SRR10533463_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10533463_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64580404
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG899710513.931633193251624No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGT51822858.024547198558869No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA8995931.3929813755887932No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT8827951.3669703893459693Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC6633701.027200139534587No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACGT5454260.8445688881103934No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA4977300.7707136672604278No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG4134340.6401849081030834Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAGATCGGAAGAGCACAC4065980.6295996537897162No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT3385660.5242550046605469Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACGTC2903850.44964878200514197No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAG2615660.405023790188739No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTCTG2377640.36816740880097315Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTCT2358610.3652206945004556Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTTAACACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGC2307080.3572414938748293No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGTC2305900.35705877590979457No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAAGATCGGAAGAGCACA2119920.32826056647152596No Hit
CTTAACACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCA2037240.31545792126044925No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA1854100.2870994737041286Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC1833440.2839003608586902No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA1813230.28077092859313796No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGA1792050.2774912959664978No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC1619300.2507416955768812Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT1535050.23769594256486842No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCTGAAGAGCACACG1497140.2318257408237954No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC1439440.22289114202506383No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACAC1422440.22025876456269922No Hit
GGGAGGGCGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACAC1411790.21860965750539435No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG1393430.21576668984604058Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGTCT1307070.20239421233722849Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTAACACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCAC1268060.19635368028976716No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACG1209140.18723016969667763No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG1184410.18340083471760257No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA1149780.1780385269810328No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAGC1148940.1779084565652454No Hit
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGGAGATCGGAAGAGCACACG1147960.17775670774682673No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACAGATCGGAAGAGCAC1127650.17461179090796647No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA1060490.16421235147429553No Hit
GGGCGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTCT1054350.16326159867318266Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTGCGGAAGGATCATTAACGGAGCAGATCGGAAGAGCACACGTCTGAAC1040900.16117892356325303Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA1037090.16058896132021722Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAGA1020290.15798755300446865No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCAGATCGGAAG1017270.15751991889056624No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG1009790.15636167280712585Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGAGATCGGAAGAGCACACGT997430.1544477795462537No Hit
CCGTCTCCGCCCCCCGGCCCCGCGTAGATCGGAAGAGCACACGTCTGAAC944300.14622082574769893Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC868260.13444635620427522No Hit
ATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACACGTCTGA863110.13364890067891183Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGCGGCGGCGGTCGGCGGGTGGCGGGGCGGAGATCGGAAGAGCACACG854610.13233271194772953No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT846820.1311264636870342No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA832320.1288812005573703Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA826230.1279381900429115No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGTAAGAGCACACGT782630.12118691608061169No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC777970.12046533496445765No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCAC719930.11147808861647877No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACA719330.1113851811766306No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAGATCGGAA711240.11013247919601121No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGAGATCGGAAGAGCACACGTCTG708810.10975620406462616Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTC691070.10700924075978217No Hit
GGATTCAACCCGGCGGCGGGTCCGGCCGTGTAGATCGGAAGAGCACACGT679400.10520219105473542No Hit
GGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAAC677910.10497147091244581Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCTTGCGACCCGGGTTCGATTCCCGGGCGGCGAGATCGGAAGAGCACAC664550.10290273191849342No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)