FastQCFastQC Report
Tue 25 Jul 2023
SRR10533464_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10533464_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41378873
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGT10460392.527954301703674No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG4251461.0274470259255248No Hit
TCGCTTCTGGCGCCAAGCGCCCGGAGATCGGAAGAGCACACGTCTGAACT4068720.983284392496625Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT3649120.8818799874032335No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACG3492870.8441191716362116No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCAGATCGGAAGAGCACACGTCTGAAC3224880.7793542371248245Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT3007200.7267476811173663No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC2983700.7210684544260062No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG2800670.6768357369230429TruSeq Adapter, Index 8 (100% over 49bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC2167420.5237987027824562No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGAGATCGGAAGAGCACACGTCTGAAC1950860.47146281630241593Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC1820280.4399056494361265No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAG1623150.392265395918347No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACGT1457720.352286056703381No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGA1398930.33807832320614434No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA1183750.2860759402509585No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG1139780.27544974460759236No Hit
AGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTC1099900.2658119760777439Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCGCTTCTGGCGCCAAGCGCCCGGCAGATCGGAAGAGCACACGTCTGAA1091000.26366112001165426Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG1066510.2577426407915943No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC1065000.2573777202680218No Hit
AACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG1056180.25524619773960494Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA1018660.2461787685711015Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG961350.23232870552081009Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGTC924200.22335069396404295No Hit
AACCCGGCGGCGGGTCCGGCCGTGAGATCGGAAGAGCACACGTCTGAACT922410.22291810605861595Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGTCCGGCCGTGAGATCGGAA893750.21599186618736574No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA887780.21454910093853932No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA833980.20154729685363834Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGTCTCCGCCCCCCGGCCCCGCGAGATCGGAAGAGCACACGTCTGAACT811350.19607832238446898Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA794260.1919481953991352Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC762110.18417852994691278No Hit
CGCTTCTGGCGCCAAGCGCCCGGCAGATCGGAAGAGCACACGTCTGAACT759290.18349702274394955Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC757870.18315385245025884Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT741330.17915664353642496No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACAC713470.1724237390419019No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT693230.16753235401070493No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA675400.16322339180189852No Hit
GAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAA668410.16153412394774502Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACG643450.15550206019385787No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA636150.1537378748812226No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGAGATCGGAAGAGCACACGT635140.15349378896810456No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA621520.1502022541793248No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCAGATCGGAAG607680.14685755216194504No Hit
GTCCGGTGCGGAGTGCCCTTCGTCCAGATCGGAAGAGCACACGTCTGAAC587250.1419202499787754Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCAGATCGGAAGAGCACACGTC564610.136448858817397No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACAC563880.13627244028613347No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC548020.13243956644251767No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACGT536250.1295951197124194No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG527690.1275264311814389Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACACGTCTGA519990.12566557818044005Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC507100.1225504619229238No Hit
CTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAAC502510.12144120019895177Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGATTCAACCCGGCGGCGGGTCCGGCCGTGAGATCGGAAGAGCACACGTC500420.12093611152725209No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACAGATCGGAAGAGCAC492750.11908250860288051No Hit
TAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACT489930.1184010013999173Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAGC488420.1180360808763448No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT484880.11718057183432715Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA466050.11262994040461179No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT465080.11239552126033012Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC459090.11094792262708555No Hit
GCTTAACACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGC456580.11034133288260412No Hit
GTCCGGTGCGGAGTGCCCTTCGTCAGATCGGAAGAGCACACGTCTGAACT442060.1068322957950063Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTCTG431100.10418360113384431Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGATTCAACCCGGCGGCGGGTCCGGCCGTGTAGATCGGAAGAGCACACGT429330.10375584661283549No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT426220.10300425533580869No Hit
CGGCCGGGCGCGACCCGCTCCGGGGACAGATCGGAAGAGCACACGTCTGA419800.1014527389375733Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)