FastQCFastQC Report
Tue 18 Jul 2023
SRR10533465_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10533465_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52438781
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACG16268033.102289887325947No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGT14972712.855274229200713No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACGT7339661.3996625894106882No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG6679291.273730981656496No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT6055201.154717917641907No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC5305491.0117493005796607No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCAGATCGGAAGAGCACACGTCTGAAC4833920.9218215808639793Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGTCCGGCCGTGAGATCGGAA3734550.7121733054778676No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT3733740.7120188396446516No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA3357990.6403638559027526No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA3217720.6136145689580389No Hit
TCGCTTCTGGCGCCAAGCGCCCGGAGATCGGAAGAGCACACGTCTGAACT3119760.5949337380668708Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC3047070.5810718597749249No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC2766240.5275179833032351No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC2735180.5215948860443571No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACAC2582830.49254196050056925No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC2507870.4782471964784994No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC2371860.45231028539736656No Hit
AACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG2194660.41851850064935725Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA2155940.4111346524245101No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT1879200.35836073306128147No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG1855860.35390982868194437TruSeq Adapter, Index 10 (100% over 49bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC1828230.3486408274822407No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAG1773430.33819054642021523No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGTCCGGCCGTGAGATCGGA1591790.30355206006791047No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC1569560.2993128310896472No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG1543560.2943546685419709No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACG1458300.2780957093567831No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT1416620.2701473933957389Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGCTTCTGGCGCCAAGCGCCCGGAGATCGGAAGAGCACACGTCTGAAC1294630.24688407611916074Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGTC1291430.2462738407286775No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGA1289020.24581425720021982No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGAGATCGGAAGAGCACACGTCT1250170.23840561816263428Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA1193650.22762733557822407Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGAGATCGGAAGAGCACACGT1193520.2276025447654857No Hit
GAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAA1184840.22594728126879993Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA1123550.21425936655545064No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA1116730.21295880237948323Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC1105590.21083442042636344No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA1074820.20496662574974808Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTG1024630.19539546504713756Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT951500.18144967938900028No Hit
CGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGA943760.17997367253826896Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG895810.17082967660899667Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCAGATCGGAAG885840.16892841197052236No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA827480.1577992440365843Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTC824220.1571775667325295Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC815760.15556425691893944No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCAGATCGGAAGAGCACACGTC806210.15374308567546602No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACGT797580.15209735710675656No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCAGATCGGAAGAGCACACGTCTGAA789480.15055269877459584Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGATTCAACCCGGCGGCGGGTCCGGCCGTGAGATCGGAAGAGCACACGTC787920.15025520902173528No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC755450.14406322679392566Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCA750510.14312117590986717No Hit
AGTCCGCCCGGAGGATTCAACCCGAGATCGGAAGAGCACACGTCTGAACT727110.1386588296169585Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGAGATCGGAAGAGC725640.13837850273445523No Hit
TCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACGTC723930.13805240819766576No Hit
AGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACGTCTG716140.13656686641895813Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGCAGATCGGAAGAG677560.1292097159924446No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAGATCGGAAGAGCA669350.12764408081873604No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA667790.12734659106587548No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA661830.12621002765110043No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAGATCGGAAGAGCACAC650110.12397504053345558No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGTAGATCGGAAGAGCACAC647770.12352880590416469No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAGC644780.12295861721118194No Hit
AGCTGGGGCGATCCACGGGAAGGGCCCGGCTCGCGTCCAGATCGGAAGAG606590.11567583922288352No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGAGATCGGAAGAGCACACGT583330.11124019072830851No Hit
GATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTCC572270.10913106465995082Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGCTTGGGCTGCAGGTCGACCCGTGCAGATCGGAAGAGCACACGTCTGA567520.10822524650220225Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC561260.10703147351956942Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGGAGGACAGAAACCTCCCGTGGAGCAGATCGGAAGAGCACACGTCTGA553820.1056126762366959Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTGA548850.1046649043958516Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGCCGGGCGCGACCCGCTCCGGGGACAGATCGGAAGAGCACACGTCTGA548610.10461913674156537Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCCGCCCGGAGGATTCAACCCGGCGGCGGAGATCGGAAGAGCACACGTC545460.10401843627905842No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT543120.10357220164976756Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCAGATCGGAAGAGCACACGTCTG543060.10356075973619601Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG530980.10125712113712176Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACCCGGCGGCGGGTCCGGCCGTGAGATCGGAAGAGCACACGTCTGAACT526090.10032460518103958Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)