FastQCFastQC Report
Thu 24 Aug 2023
SRR10533467_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10533467_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40303232
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGT27755586.886688392633126No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG23236755.765480545083829No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG9070872.2506557290492237TruSeq Adapter, Index 1 (100% over 49bp)
AACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG7832791.9434644844363846Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC3554010.8818176170089783No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACG3103540.7700474244844682No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC2953970.7329362568242667No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT2783050.6905277472536198No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC2254120.5592901333570469No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGTC2243260.5565955603759024No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACGT2207270.5476657554411517No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAG1912670.4745698806487777No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA1684940.4180657273342247Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG1683920.41781264589400674Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT1355140.33623606166373954Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA1315790.32647257669062374No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA1261290.31295008797309354Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT1244480.30877920659067737No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT1192460.2958720531395596No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT1183400.29362409446468213No Hit
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC1180470.292897105621703Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA1178910.2925100398896049No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA1141650.2832651237498769No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA1120070.2779107144558531Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTAACACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCA1099490.2728044242208664No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT1096440.27204766109080286Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACGT1069720.2654179198333275No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG1039470.25791231829745065Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACGTC1032470.25617548488418995No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA992790.24633012062159182Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCTTAACACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGC992450.2462457601415192No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC976320.24224359971924833No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC975160.2419557816107651No Hit
TTAACACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCAC940640.2333907116927992No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA927670.23017260749708607No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG925460.22962426437661376No Hit
GGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGTCT898150.22284813287430644Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT797330.19783276934217087Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC761730.18899973084044477No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACAC737080.18288359603517654No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC716320.1777326443695632No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAGATCGGAAGAGCACAC707080.17544002426405902No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC697070.17295635248309613No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT686570.170351102363205No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTAGATCGGAAGAGCACACGTCT661280.16407617136015296Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCAGATCGGAAG641030.15905176041464864No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAGC627700.1557443333576821No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG614590.15249149249370375No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC608750.1510424771889262No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTAGATCGGAAGAGCACACGTC606310.1504370666848753No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGA593190.14718174463030656No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAAGATCGGAAGAGCACA556700.1381278801660373No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCAGATCGGAAGAGCACACGTC554570.13759938657028797No Hit
TTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAGA548610.13612059697842596No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA545500.1353489467048201No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC539700.13390985616240403No Hit
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA533680.1324161794269998Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTGA521950.12950574286449285Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGATTCAACCCGGCGGCGGGTCCGGCCGTGAGATCGGAAGAGCACACGTC514700.1277068796864728No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGTCCGGCCGTGAGATCGGA507740.1259799710355735No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGAGATCGGAAGAGCACACGTCT495680.12298765518358429Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGAGATCGGAAGAGCACACGT482040.11960331121831619No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA475860.11806993543346599No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCAGATCGGAAGAGCACACGTCTGAA454890.11286687876545483Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTCTGAACTC449150.11144267536658103Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGAGATCGGAAGAG422060.1047211300572619No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGTAAGAGCACACGT407640.10114325322594475No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)