FastQCFastQC Report
Tue 25 Jul 2023
SRR10533468_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10533468_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38841828
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG36741689.459307630938483TruSeq Adapter, Index 7 (100% over 49bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC11588122.9834126241432304No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT10129752.607948832892211No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT6805831.752190962794027No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG5160551.3286063673419284No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCAC3690340.9500943158493983No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC2719990.7002734268840283No Hit
CGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCA2705070.696432207052665No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC2618600.6741701240219693No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC2488290.6406212395564905No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC2237210.5759795857187772No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC2117200.5450824816998828No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT2016200.519079586058617No Hit
GCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAACT1882200.484580694811789Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAACTCC1826140.4701478004588249Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT1752890.4512892647586Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC1681790.4329842560447979No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACG1534940.3951770756000464No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAGATCGGAAGAGCA1381100.35557028881338953No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGAGATCGGAAGAGCACACGTCT1367890.35216931602704177Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACG1257470.3237411998220063No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAG1185330.30516843851942294No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA1159440.298502943785241Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA964390.24828646066812304No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGT960250.24722059940124344No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA953160.24539524761810905No Hit
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCA952360.2451892840882772No Hit
GGACGGTGTGAGGCCGGTAGCGGCCCCCGAGATCGGAAGAGCACACGTCT939330.2418346530961416Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACA911350.2346310786402741No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCG898410.23129961854524458TruSeq Adapter, Index 7 (100% over 50bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACG896930.23091858601505572No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC855300.22020075883143297No Hit
CGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAAC846240.21786822185608773Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTTAACACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCA845970.21779870916476948No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACAC838840.21596305920514347No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG833550.21460112536413065No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACG827270.21298431165495094No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA815610.20998239320765233No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACAC801850.2064398204945452No Hit
ACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAACTCCA781390.20117230321909668Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GACGGCGCGACCCGCCCGGGGCGCACTGGGGACAGATCGGAAGAGCACAC778450.2004153872469648No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT775900.1997588784956259No Hit
CGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAACTC775060.1995426167893025Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC768520.19785886493292748No Hit
AACCCGGCGGCGGGTCCGGCCGTGAGATCGGAAGAGCACACGTCTGAACT761430.19603351314979306Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGCGGTGCGCCGCGACCGGCTCCGAGATCGGAAGAGCACACGTCTGAACT757730.19508093182432093Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC756570.19478228470606482TruSeq Adapter, Index 7 (100% over 50bp)
ACCGCGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAG754370.19421588499902734No Hit
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC736930.1897258800486939Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC728330.18751177210300196TruSeq Adapter, Index 7 (100% over 50bp)
GCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT709650.1827025236814292Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCAGATCGGAAG700570.18036483761783817No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT696280.1792603581891151No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT672570.17315611407372483Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACGT670230.17255367074896683No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA637110.16402678061392992No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT630460.162314708772203Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACGGTGTGAGGCCGGTAGCGGCCCCCGAGATCGGAAGAGCACACGTCTG607710.15645762089261092Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC604220.15555910499371967No Hit
TCAACCCGGCGGCGGGTCCGGCCGTGAGATCGGAAGAGCACACGTCTGAA591460.15227398669290232Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGT582600.149992940600015No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCAGATCGGAAGAGCACACGTCTG581560.14972518801123366Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGT581390.1496814207611444No Hit
GCTTAACACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGC568180.14628044797479664No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGTC561370.14452718342710336No Hit
TTAACACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCAC557750.14359519845461444No Hit
CCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTGAA556710.1433274458658331Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA550380.1416977594360389Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC546600.14072458175758362Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAAGATCGGAAGAGCACAC545820.14052376731599758No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT532190.13701466367648812No Hit
TTCAACCCGGCGGCGGGTCCGGCCGTGAGATCGGAAGAGCACACGTCTGA529550.13633498402804317Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACG514200.13238305879939533No Hit
ACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTCTGAACTC509500.13117302306163345Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTC499840.12868601343891437No Hit
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAGATCGGAAGAGCAC485170.12490915721062355No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCAGATCGGAAGAGCACACGT465620.11987592345035873No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACG464100.11948459274367829No Hit
CGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCAC444320.1143921444685868No Hit
CACCGCGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAA441700.11371761390838762No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGT432310.11130011697698676TruSeq Adapter, Index 7 (100% over 50bp)
AGCTGGGGCGATCCACGGGAAGGGCCCGGCTCGCGTCCAGATCGGAAGAG428860.11041189925458708No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG416770.10729927541000388No Hit
AACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTCTGAACTCCAGT413610.10648571946716823Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA413460.10644710130532477Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACAGATCGGAAGAGCACA412830.10628490502558222No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGA411510.10594506520135974No Hit
GGATTCAACCCGGCGGCGGGTCCGGCCGTGAGATCGGAAGAGCACACGTC395380.10179232553112587No Hit
CGACGGCGCGACCCGCCCGGGGCGCACTGGGGACAGATCGGAAGAGCACA394700.10161725653076883No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACA391040.10067497338178831No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)