FastQCFastQC Report
Tue 18 Jul 2023
SRR10533469_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10533469_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58391812
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGT12627482.1625429263952283No Hit
AACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG9792171.676976559658741Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG7184631.2304173742715845No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC5922941.0143442714194242No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC5505170.9427982813754778No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG4228920.7242316782359828Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACG4186680.7169977872925061No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA4026320.6895350327542499Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTAACACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCA3746570.641625918373624No Hit
GCTTAACACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGC3681670.6305113463510946No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT3657290.6263361034249116No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA3293310.5640020213792989Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC2921340.5002995967996334No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA2747020.47044609610676236Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC2565710.4393955097677051Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTAACACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCAC2506580.42926908998816476No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA2504640.428936851625704Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA2453970.4202592651175134No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA2420070.4144536566188424No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT2299260.3937641119957024Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG2183380.3739188638297438Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT2034270.34838274927998464No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC2031960.34798714586901325No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT1879150.32181738083414846Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA1845490.31605287398856535No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC1817900.3113278964523314No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACGT1809680.30992016483406953No Hit
TTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAGA1581020.27076056485453814No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG1442850.24709800065803744TruSeq Adapter, Index 11 (100% over 49bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAG1417380.2427360877240802No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC1291020.2210960673732817No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC1257240.21531100970115466No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA1199460.205415786720234No Hit
TTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGAGATCGGAAGAG1164170.1993721311474287No Hit
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA1132880.19401350312608898Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTGCGGAAGGATCATTAACGGAGCAGATCGGAAGAGCACACGTCTGAAC1127020.19300993776319186Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGAGATCGGAAGA1118210.19150116458108885No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT1090930.18682927668009344Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA1060940.18169328261298004No Hit
ATTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGAAC1007190.17248822489016097Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGA1000690.17137505511902934No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGTCCGGCCGTGAGATCGGAA985840.16883189033421328No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT974500.1668898372258083No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC973580.1667322808889712No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC945870.16198675252619324No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGAGATCGGAAGAGCACACGT923770.15820197530434574No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACAC897900.15377155961524194No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGTC895650.15338623161754256No Hit
AGCTGGGGCGATCCACGGGAAGGGCCCGGCTCGCGTCCAGATCGGAAGAG859380.14717474429462818No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGATCGGAAGAG828490.14188461902843502No Hit
AACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTCTGAACTCCAGT780580.1336797015307557Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG778830.13338000197698952No Hit
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCA764420.13091219022283465No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT759760.13011413312537723No Hit
CCTGCGGAAGGATCATTAACGGAGAGATCGGAAGAGCACACGTCTGAACT755740.1294256804361543Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA736000.12604506946967153No Hit
TTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGAACT728780.12480859473927612Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCGCTTCTGGCGCCAAGCGCCCGGCAGATCGGAAGAGCACACGTCTGAA655740.11229999164951415Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACAC653970.1119968669579906No Hit
ATTGATCATCGACACTTCGAACGCACAGATCGGAAGAGCACACGTCTGAA620170.10620838414810625Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAACT616430.10556788338748592Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACGT613490.10506438813715868No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAG598670.10252636105897862No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACG595110.1019166865381742No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)