FastQCFastQC Report
Tue 18 Jul 2023
SRR10603617.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10603617.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4866597
Total Bases248.1 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG2383574.89781668792382TruSeq Adapter, Index 7 (100% over 49bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG1610603.309499430505546No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGT1506013.0945853950922997No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA1147342.3575816941489096Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC809211.6627840768405522No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA609071.2515316143909183Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTG439180.9024375759899577Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACAC432410.8885264179466678No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTC428410.8803071222046945No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACGT404500.8311762819070492No Hit
GACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA363750.7474422065356963Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACG311050.6391529851351982No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCT275860.5668437308451881Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACACG270550.5559326157477186No Hit
GACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTG269220.5531996999135125Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC254200.5223362444024027No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTG236010.4849589970157792Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA213580.4388692961426639No Hit
ACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTGA213320.4383350419194357Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACAC199630.4102045022425321No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA190320.3910740914030893Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT187860.3860192245217757No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTG185140.3804301034172339Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG184220.37853966539657996No Hit
CGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGCACA178300.3663751076984595No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA176590.36286135876876596Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTAAAATTGGAACGATACAGAGAAAGATCGGAAGAGCACACGTCTGAACT171620.35264888380936416Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA170760.35088173522483984No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC168460.3461556401732052No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCAC157540.32371696279761814No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCT149180.30653863469689396Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC149060.30629205582463476No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGAGATCGGAAGAGCACACGT143740.29536039248781026No Hit
TAAAATTGGAACGATACAGAGAAAGATCGGAAGAGCACACGTCTGAACTC139720.2871000002671271Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGTCGCGGCGTGGGAAATGTGGCGTACGGAGATCGGAAGAGCACACGTCT138810.2852301104858282Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCAGATCGGAAGAGCACAC130640.26844219893284776No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA129720.26655176091219385No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACA129670.26644901971541923No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCAC127610.26221608240830296No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGGAAGAGATCGGAAGAGCACACG127100.26116812220120134No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACAC121350.24935288457211477No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACGTC119260.24505830254693373No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTGA117420.24127742650562603Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGAGATCGGAAGAGCA109370.22473609382490473No Hit
TTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTC106540.21892094208745866No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC105290.216352412168092No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACA99170.20377688968287286No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC97160.1996466935725313Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGT94900.19500279147831637No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT94110.19337948056927665No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAGATCGGAAGAGCA92810.19070820945313532No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC92220.18949586333119425No Hit
CGGAATGCCTAAAGGAAAGATCCAAAAAGATAAGATCGGAAGAGCACACG89610.1841327728595567No Hit
CGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTCTG89570.18405057990213697Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGAATGCCTAAAGGAAAGATCCAAAAAGATAAGATCGGAAGAGCACACGT84580.17379700846402527No Hit
CGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTCTGAAC82180.1688654310188413Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAAGATCGGAAGAGCACACGT79930.16424207716398131No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGGAAGATCGGAAGAGCACACGTC78160.16060503879815816No Hit
GACGTACGCAGTTTTATCCGGTAAAGCGAAGATCGGAAGAGCACACGTCT76470.15713238634717444Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAAGATCGGAAGAGCACACGTC71840.14761855152584033No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG71340.1465911395580937No Hit
AAAATTGGAACGATACAGAGAAAGATCGGAAGAGCACACGTCTGAACTCC70900.1456870170264766Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACTAAAATTGGAACGATACAGAGAAAGATCGGAAGAGCACACGTCTGAAC68860.14149517619807023Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTCGCGGCGTGGGAAATGTGGCGTACGGAAAGATCGGAAGAGCACACGTC68180.14009789592193478No Hit
TCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTCT65540.1346731607322324Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGC62430.12828265829284816No Hit
CGCGGCGTGGGAAATGTGGCGTACGGAAGAGATCGGAAGAGCACACGTCT60010.12330998436895432Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA57390.11792634565796181Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA57020.11716606080182927No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGAAGATCGGAAGAGCACACG55590.11422766257407384No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC54860.1127276411011637Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGTCCGGAGATCGGAAGAGC54360.11170022913341705No Hit
ACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGAA53490.10991253230953786Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC53000.10890566858114614No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT52510.10789880485275441No Hit
TGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCA52480.1078371601346896No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCAGATCGGAAGAGCACA51300.10541246789080748No Hit
GCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACA51070.10493985838564401No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGCAC50780.10434395944435096No Hit
GCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTCTGA50610.10399463937531708Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTCT49860.10245352142369708Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTC49250.10120007882304617No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)