FastQCFastQC Report
Tue 18 Jul 2023
SRR10620357_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10620357_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37833254
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length49
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGACCGACGCGGATTACGGTGGCGGCCTAAGATCGGAAGAGCACACGT25101686.634819199004135No Hit
GACCGACGCGGATTACGGTGGCGGCCTAAGCCCGAGATCGGAAGAGCAC17775224.6983058871964865No Hit
ACCGACGCGGATTACGGTGGCGGCCTAAGCCCGAGATCGGAAGAGCACA10643132.813168013515306No Hit
CCGACGCGGATTACGGTGGCGGCCTAAGCCCGAGATCGGAAGAGCACAC9262752.4483090986569644No Hit
CTGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGT8111542.1440238790985306No Hit
GTGGACCGACGCGGATTACGGTGGCGGCCTAAGATCGGAAGAGCACACG5557161.468855943504093No Hit
TGTGGAGACGTCGCTGCCGTGATCGTGGTCTAGATCGGAAGAGCACACG4053101.0713062111971654No Hit
TCTGATGATTCATGATAACTCGACGGATCGCATAGATCGGAAGAGCACA3555510.939784349503746No Hit
GGACCGACGCGGATTACGGTGGCGGCCTAAGCCCGAGATCGGAAGAGCA3120950.8249224346391141No Hit
GGACCGACGCGGATTACGGTGGCGGCCTAAGCCCAGATCGGAAGAGCAC2918610.7714403841657395No Hit
GACCGACGCGGATTACGGTGGCGGCCTAAGCCCAGATCGGAAGAGCACA2295860.6068365147761279No Hit
GGACCGACGCGGATTACGGTGGCGGCCTAAAGATCGGAAGAGCACACGT2147480.5676170492762795No Hit
CGACGCGGATTACGGTGGCGGCCTAAGCCCGAGATCGGAAGAGCACACG2130450.5631157182514621No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTAT2088840.5521174573035669TruSeq Adapter, Index 4 (100% over 48bp)
GGACCGACGCGGATTACGGTGGCGGCCTAAGATCGGAAGAGCACACGTC2008070.5307685138582052No Hit
TCTGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACG1806050.4773710450599888No Hit
TGGAGACGTCGCTGCCGTGATCGTGGTCTAGATCGGAAGAGCACACGTC1786540.4722142060526964No Hit
AAGAAGGGGTAGAGAAAATGCCCCGAGCCAATGTTCAGATCGGAAGAGC1655790.43765466221858684No Hit
CGTAGGTTCGAATCCTACTTGGGGAAGATCGGAAGAGCACACGTCTGAA1531440.4047867518876383Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGACCGACGCGGATTACGGTGGCGGCCTAAAGATCGGAAGAGCACACG1510200.39917264319902274No Hit
TGGACCGACGCGGATTACGGTGGCGGCCAGATCGGAAGAGCACACGTCT1506810.3982766060778171Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTC1409340.37251355646014483No Hit
GTGGACCGACGCGGATTACGGTGGCGGCCAGATCGGAAGAGCACACGTC1356040.3584254211916321No Hit
TGTGGAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGT1237530.327101126432318No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACTCGGAGATCGGAAGAGCACAC1204720.31842886155126915No Hit
TGGACCGACGCGGATTACGGTGGCGGCCTAAGAGATCGGAAGAGCACAC1116170.2950235261286275No Hit
CCGACGCGGATTACGGTGGCGGCCTAAGCCCAGATCGGAAGAGCACACG1026870.27141995240483413No Hit
GGACCGACGCGGATTACGGTGGCGGCCTAAGCAGATCGGAAGAGCACAC950060.2511177071895534No Hit
GTGGAGACGTCGCTGCCGTGATCGTGGTCTAGATCGGAAGAGCACACGT934580.2470260686537827No Hit
TTGGACCGACGCGGATTACGGTGGCGGCCTAAGATCGGAAGAGCACACG886940.234433971764628No Hit
TGGACCGACGCGGATTACGGTGGCGGCCTAAGCAGATCGGAAGAGCACA815870.21564891034749484No Hit
TCTGATGATTCATGATAACTCGACGGATCGCAAGATCGGAAGAGCACAC799200.21124273370723015No Hit
ACCGACGCGGATTACGGTGGCGGCCTAAGCCCAGATCGGAAGAGCACAC791070.20909383052274594No Hit
TGGACCGACGCGGATTACGGTGGCGGCCTAAGCCCAGATCGGAAGAGCA783050.2069740022890973No Hit
AAATCGAGGCAAACTCTGAATACTAGATAAGATCGGAAGAGCACACGTC776040.2051211349676663No Hit
CGGATTACGGTGGCGGCCTAAGCCCGAGATCGGAAGAGCACACGTCTGA752380.19886737736066795Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTCATGATAACTCGACGGATCGCATAGATCGGAAGAGCACACGTCTGA744070.19667089698390733Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGACCGACGCGGATTACGGTGGCGGCCTAAGAGATCGGAAGAGCACACG743390.19649116092419647No Hit
GACCGACGCGGATTACGGTGGCGGCCTAAGCAGATCGGAAGAGCACACG701380.1853871728823537No Hit
CTGATGATTCATGATAACTCGACGGATCGCAAGATCGGAAGAGCACACG619040.16362325059324792No Hit
GGAGACGTCGCTGCCGTGATCGTGGTCTAGATCGGAAGAGCACACGTCT596090.15755715857800653Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTCT562100.1485729987698124Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGATGATTCATGATAACTCGACGGATCGCATAGATCGGAAGAGCACAC558880.14772189566353452No Hit
CGTGGACCGACGCGGATTACGGTGGCGGCCTAAGATCGGAAGAGCACAC517470.1367764982626131No Hit
GCGGGTATAGTTTAGTGGTAAAACCTTAGCAGATCGGAAGAGCACACGT503580.13310512492528398No Hit
TGGACCGACGCGGATTACGGTGGCGGCCTAGATCGGAAGAGCACACGTC501080.13244433058811172No Hit
ATCGAGGCAAACTCTGAATACTAGATATGACCTAGATCGGAAGAGCACA489890.12948661513492865No Hit
ATTACGGTGGCGGCCTAAGCCCGAGATCGGAAGAGCACACGTCTGAACT481780.1273429983051418Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AATCGAGGCAAACTCTGAATACTAGATAAGATCGGAAGAGCACACGTCT465850.12313241678868014Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTCTG446760.11808659123003272Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGACCGACGCGGATTACGGTGGCGGCCTAAAGATCGGAAGAGCACAC446600.11804430039245369No Hit
TCGAGGCAAACTCTGAATACTAGATATGACCTCAGATCGGAAGAGCACA444790.11756588529234097No Hit
GTCTAGTGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACT443090.11711654514306381Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCGACGCGGATTACGGTGGCGGCCTAAGCAGATCGGAAGAGCACACGT439670.11621257848981217No Hit
TCCGATGTCGTCCAGCGGTTAGGATATCTGGAGATCGGAAGAGCACACG433420.1145605926468815No Hit
TGGAGACGTCGCTGCCGTGATCGTGGTCTGAGATCGGAAGAGCACACGT409800.10831740774927792No Hit
ATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTCTGAAC399720.10565308498179934Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGCTGCCGTGATCGTGGTCTAGATCGGAAGAGCACACGTCTGAACTCC394350.10423369874555331Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)