FastQCFastQC Report
Tue 18 Jul 2023
SRR10620358_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10620358_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53977204
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length49
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGACCGACGCGGATTACGGTGGCGGCCTAAGATCGGAAGAGCACACGT9580951.7749993126728092No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTAT8785571.6276445145250575TruSeq Adapter, Index 6 (100% over 48bp)
GACCGACGCGGATTACGGTGGCGGCCTAAGCCCGAGATCGGAAGAGCAC7768721.4392594325560102No Hit
ACCGACGCGGATTACGGTGGCGGCCTAAGCCCGAGATCGGAAGAGCACA4977720.9221893005054503No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACTCGGAGATCGGAAGAGCACAC4618320.855605636779556No Hit
TCTGATGATTCATGATAACTCGACGGATCGCATAGATCGGAAGAGCACA4125130.7642355835993283No Hit
CGTAGGTTCGAATCCTACTTGGGGAAGATCGGAAGAGCACACGTCTGAA3427960.635075503355083Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGACGCGGATTACGGTGGCGGCCTAAGCCCGAGATCGGAAGAGCACAC3114060.5769213240463511No Hit
GGACCGACGCGGATTACGGTGGCGGCCTAAGCCCGAGATCGGAAGAGCA2880880.5337216058838469No Hit
CTGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGT2838930.5259498065146168No Hit
TCCGATGTCGTCCAGCGGTTAGGATATCTGGAGATCGGAAGAGCACACG2506370.4643386122778793No Hit
GTGGACCGACGCGGATTACGGTGGCGGCCTAAGATCGGAAGAGCACACG1874940.3473577475409805No Hit
TCCGATGTCGTCCAGCGGTTAGGATATCTGGCAGATCGGAAGAGCACAC1465060.27142198769688036No Hit
TGGACCGACGCGGATTACGGTGGCGGCCTAAGCCCAGATCGGAAGAGCA1201140.22252727280946233No Hit
GTCTAGTGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACT1144090.21195799619409703Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCGGGTATAGTTTAGTGGTAAAACCTTAGCAGATCGGAAGAGCACACGT1103890.20451040776398866No Hit
ATTCATGATAACTCGACGGATCGCATAGATCGGAAGAGCACACGTCTGA1059420.1962717446424235Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGTTATCGTCCAGCGGTTAGGATATCTGGAGATCGGAAGAGCACACG1057280.1958752809797262No Hit
CTCTGATGATTCATGATAACTCGACGGATCGCATAGATCGGAAGAGCAC942400.17459222230184432No Hit
TGGACCGACGCGGATTACGGTGGCGGCCTAAGCCCGAGATCGGAAGAGC853530.15812786449627886No Hit
TGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTC732320.13567208853574558No Hit
GGACCGACGCGGATTACGGTGGCGGCCTAAAGATCGGAAGAGCACACGT662300.12269994570300455No Hit
TGGACCGACGCGGATTACGGTGGCGGCCTAAGCAGATCGGAAGAGCACA623250.11546541017574752No Hit
TGGACCGACGCGGATTACGGTGGCGGCCTAAAGATCGGAAGAGCACACG608070.11265311185810957No Hit
CGGATTACGGTGGCGGCCTAAGCCCGAGATCGGAAGAGCACACGTCTGA601040.11135071019980955Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGACCGACGCGGATTACGGTGGCGGCCTAAGCCCAGATCGGAAGAGCAC592810.10982599246896893No Hit
AGCTAACGATGCGGGTTCGATTCCCGCTACCCGCAGATCGGAAGAGCAC591200.10952771840497703No Hit
TCCGTTATCGTCCAGCGGTTAGGATATCTGGCAGATCGGAAGAGCACAC589840.10927576018943108No Hit
GACCGACGCGGATTACGGTGGCGGCCTAAGCCCAGATCGGAAGAGCACA585960.10855693822154996No Hit
GTCTAGTGGTAGAATAGTACCAGATCGGAAGAGCACACGTCTGAACTCC572220.10601141919096069Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCGGGTTCGAGTCCCGGGCAACCAGATCGGAAGAGCACACGTCTGAAC547590.10144838180206593Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)