FastQCFastQC Report
Tue 18 Jul 2023
SRR10620359_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10620359_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38730358
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length49
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCGACGCGGATTACGGTGGCGGCCTAAGCCCGAGATCGGAAGAGCAC13987353.6114693285303487No Hit
ACCGACGCGGATTACGGTGGCGGCCTAAGCCCGAGATCGGAAGAGCACA11026602.847017319075646No Hit
CTGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGT10040722.5924676451480257No Hit
TGGACCGACGCGGATTACGGTGGCGGCCTAAGATCGGAAGAGCACACGT8453182.182572131143224No Hit
TCTGATGATTCATGATAACTCGACGGATCGCATAGATCGGAAGAGCACA5825791.5041921378573366No Hit
CCGACGCGGATTACGGTGGCGGCCTAAGCCCGAGATCGGAAGAGCACAC5544191.4314843152237322No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTAT4306411.1118952218308955TruSeq Adapter, Index 8 (100% over 48bp)
ATTCATGATAACTCGACGGATCGCATAGATCGGAAGAGCACACGTCTGA3852620.9947287344981423Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGACCGACGCGGATTACGGTGGCGGCCTAAGCCCGAGATCGGAAGAGCA3495020.9023980620060367No Hit
TGTGGAGACGTCGCTGCCGTGATCGTGGTCTAGATCGGAAGAGCACACG2518810.6503451375275179No Hit
CTCTGATGATTCATGATAACTCGACGGATCGCATAGATCGGAAGAGCAC1876640.48453980208496905No Hit
TCTGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACG1856730.47939913181282756No Hit
GGACCGACGCGGATTACGGTGGCGGCCTAAGATCGGAAGAGCACACGTC1757080.45366996091283224No Hit
TGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTC1718310.4436597255310679No Hit
GTGGACCGACGCGGATTACGGTGGCGGCCTAAGATCGGAAGAGCACACG1711490.44189883295166027No Hit
CGTAGGTTCGAATCCTACTTGGGGAAGATCGGAAGAGCACACGTCTGAA1568140.40488652338302683Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCGTTGTAGTCTAGCTGGTCAGGATACTCGGAGATCGGAAGAGCACAC1527430.39437538893908497No Hit
CGGATTACGGTGGCGGCCTAAGCCCGAGATCGGAAGAGCACACGTCTGA1275480.3293230597042248Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGACCGACGCGGATTACGGTGGCGGCCAGATCGGAAGAGCACACGTCT1191480.3076346467027235Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGAGACGTCGCTGCCGTGATCGTGGTCTAGATCGGAAGAGCACACGTC1184530.3058401887222421No Hit
AAATCGAGGCAAACTCTGAATACTAGATAAGATCGGAAGAGCACACGTC1156410.29857973427459666No Hit
AATCGAGGCAAACTCTGAATACTAGATAAGATCGGAAGAGCACACGTCT1005600.2596412870751156Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGACCGACGCGGATTACGGTGGCGGCCTAAAGATCGGAAGAGCACACGT970330.2505347355684138No Hit
GACCGACGCGGATTACGGTGGCGGCCTAAGCCCAGATCGGAAGAGCACA928830.23981962676410065No Hit
CGACGCGGATTACGGTGGCGGCCTAAGCCCGAGATCGGAAGAGCACACG927730.23953561183193817No Hit
GGACCGACGCGGATTACGGTGGCGGCCTAAGCCCAGATCGGAAGAGCAC907090.23420645892299782No Hit
TGTGGAGACGTCGCTGCCGTGATCGTGGTCTGAGATCGGAAGAGCACAC886980.22901414957228125No Hit
ATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTCTGAAC809610.20903757202554132Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGACCGACGCGGATTACGGTGGCGGCCTAAGCCCAGATCGGAAGAGCA788440.2035715755583772No Hit
ATCGAGGCAAACTCTGAATACTAGATATGACCTAGATCGGAAGAGCACA769940.19879496079018943No Hit
ATGATTCATGATAACTCGACGGATCGCATAGATCGGAAGAGCACACGTC729740.18841550599661383No Hit
TTGTGGAGACGTCGCTGCCGTGATCGTGGTCTAGATCGGAAGAGCACAC720930.18614080458538493No Hit
TCCGATGTCGTCCAGCGGTTAGGATATCTGGAGATCGGAAGAGCACACG718350.1854746604717674No Hit
ATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTCTG685680.17703941698654063Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGACCGACGCGGATTACGGTGGCGGCCTAAGCAGATCGGAAGAGCACA665540.17183936177403783No Hit
TCCGATGTCGTCCAGCGGTTAGGATATCTGGCAGATCGGAAGAGCACAC653680.16877716441454013No Hit
GCGGGTATAGTTTAGTGGTAAAACCTTAGCAGATCGGAAGAGCACACGT596280.15395674886351424No Hit
ACCGACGCGGATTACGGTGGCGGCCTAAGCCCAGATCGGAAGAGCACAC594760.1535642918663442No Hit
GAGACGTCGCTGCCGTGATCGTGGTCTAGATCGGAAGAGCACACGTCTG593430.15322089199382047Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGACGCGGATTACGGTGGCGGCCTAAGCCCAGATCGGAAGAGCACACG546920.14121222427120347No Hit
TGGACCGACGCGGATTACGGTGGCGGCCTAAAGATCGGAAGAGCACACG543860.14042214636900593No Hit
GAGACGTCGCTGCCGTGATCGTGGTCTGAGATCGGAAGAGCACACGTCT537040.13866125378959834Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTGGAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGT524720.13548028654937816No Hit
TGGAGACGTCGCTGCCGTGATCGTGGTCTGAGATCGGAAGAGCACACGT514180.13275890710847546No Hit
TTCATGATAACTCGACGGATCGCATAGATCGGAAGAGCACACGTCTGAA504920.13036801777045284Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGGACCGACGCGGATTACGGTGGCGGCCAGATCGGAAGAGCACACGTC501850.12957535791432653No Hit
TGGACCGACGCGGATTACGGTGGCGGCCTAAGCCCGAGATCGGAAGAGC493340.12737811512096014No Hit
AAGAAGGGGTAGAGAAAATGCCCCGAGCCAGATCGGAAGAGCACACGTC488410.12610521183408632No Hit
TCGAGGCAAACTCTGAATACTAGATATGACCTCAGATCGGAAGAGCACA487760.12593738482871755No Hit
GGAGACGTCGCTGCCGTGATCGTGGTCTAGATCGGAAGAGCACACGTCT482850.12466964544970123Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTGATGATTCATGATAACTCGACGGATCGCAAGATCGGAAGAGCACAC480410.1240396486910862No Hit
GTGGAGACGTCGCTGCCGTGATCGTGGTCTAGATCGGAAGAGCACACGT474300.12246207484061986No Hit
CTGATGATTCATGATAACTCGACGGATCGCATAGATCGGAAGAGCACAC471260.12167716084627982No Hit
GGACCGACGCGGATTACGGTGGCGGCCTAAGCAGATCGGAAGAGCACAC459070.11852975900713336No Hit
CTGATGATTCATGATAACTCGACGGATCGCAAGATCGGAAGAGCACACG420060.10845755673107901No Hit
ATTACGGTGGCGGCCTAAGCCCGAGATCGGAAGAGCACACGTCTGAACT417990.10792309226782773Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGCTCAGCCGGAGGTAGGGTCCAGCGGCTGGAGATCGGAAGAGCACAC415680.10732666091028645No Hit
TGGACCGACGCGGATTACGGTGGCGGCCTAAGAGATCGGAAGAGCACAC409800.10580847200018136No Hit
CGTGGACCGACGCGGATTACGGTGGCGGCCTAAGATCGGAAGAGCACAC406120.10485831295440129No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)