FastQCFastQC Report
Tue 25 Jul 2023
SRR10620360_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10620360_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44366576
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length49
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACGCGGATTACGGTGGCGGCCTAAGCCCGAGATCGGAAGAGCACAC16969813.8249086429387744No Hit
CTGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGT12872352.9013620523702346No Hit
TGTGGAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGT12014922.7081017025068603No Hit
GAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTCTGA10809592.4364264666265885Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGACGCGGATTACGGTGGCGGCCTAAGCCCGAGATCGGAAGAGCACA10139112.2853036934831303No Hit
TGTGGAGACGTCGCTGCCGTGATCGTGGTCTAGATCGGAAGAGCACACG10065072.2686154550218163No Hit
GAGACGTCGCTGCCGTGATCGTGGTCTAGATCGGAAGAGCACACGTCTG8640141.9474434989078266Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGAGACGTCGCTGCCGTGATCGTGGTCTAGATCGGAAGAGCACACGTC6782081.5286462493747546No Hit
TGGAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTCT6734361.5178904047046586Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACCGACGCGGATTACGGTGGCGGCCTAAGCCCGAGATCGGAAGAGCAC6318391.4241328877847144No Hit
TGGACCGACGCGGATTACGGTGGCGGCCTAAGATCGGAAGAGCACACGT5526731.2456967605523581No Hit
AGACGTCGCTGCCGTGATCGTGGTCTAGATCGGAAGAGCACACGTCTGA4845461.0921419764283815Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACGCGGATTACGGTGGCGGCCTAAGCCCGAGATCGGAAGAGCACACG4770601.0752689141483445No Hit
GTGGAGACGTCGCTGCCGTGATCGTGGTCTAGATCGGAAGAGCACACGT4139920.9331168580599954No Hit
GAGACGTCGCTGCCGTGATCGTGGTCTGAGATCGGAAGAGCACACGTCT3765030.8486185636682895Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGAGACGTCGCTGCCGTGATCGTGGTCTGAGATCGGAAGAGCACACGT3710180.8362556533549038No Hit
CCGACGCGGATTACGGTGGCGGCCTAAGCCCAGATCGGAAGAGCACACG3595270.8103555252945369No Hit
CCGACGCGGATTACGGTGGCGGCCTAAGCCCGAGATCGTAAGAGCACAC3225570.7270270304384093No Hit
GGACCGACGCGGATTACGGTGGCGGCCTAAGATCGGAAGAGCACACGTC2838960.6398871078083646No Hit
GGAGACGTCGCTGCCGTGATCGTGGTCTAGATCGGAAGAGCACACGTCT2661520.5998930365958374Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGACGCGGATTACGGTGGCGGCCTAAGCCCGAGATCTGAAGAGCACA2627260.5921710072916152No Hit
GTGGAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTC2562360.5775428782243642No Hit
GGAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTCTG2452350.5527471851783199Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTCTGAA2410760.5433730112506315Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAGACGTCGCTGCCGTGATCGTGGTCTAGATCGGAAGATCACACGTCTG2223790.5012309266326975Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TGTGGAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTC1940050.43727737745639866No Hit
CTGATGATTCATGATAACTCGACGGATCGCAGATCGGAATAGCACACGT1865870.4205575837089614No Hit
TCTGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACG1837720.41421271724912917No Hit
GGACCGACGCGGATTACGGTGGCGGCCAGATCGGAAGAGCACACGTCTG1824880.4113186467218024Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGTGGAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAATAGCACACGT1809600.40787461263632335No Hit
TGGAGACGTCGCTGCCGTGATCGTGGTCTAGATCGGAATAGCACACGTC1681820.37907365220160333No Hit
AGACGTCGCTGCCGTGATCGTGGTCTGAGATCGGAAGAGCACACGTCTG1591270.3586641439267254Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACGCGGATTACGGTGGCGGCCTAAGCCCAGATCGGAAGAGCACACGT1499950.3380810815781682No Hit
CGGCTCAGCCGGAGGTAGGGTCCAGCGGCTGGAGATCGGAAGAGCACAC1418850.3198015551166265No Hit
TGGAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTCTG1306700.29452351698269436Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGCTGCCGTGATCGTGGTCTAGATCGGAAGAGCACACGTCTGAACTCC1303670.2938405704330215Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCGAGGCAAACTCTGAATACTAGATAAGATCGGAAGAGCACACGTCTG1280120.28853252051724704Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGACCGACGCGGATTACGGTGGCGGCCAGATCGGAAGAGCACACGTCT1269110.28605092265853466Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGATCACACGTCT1218600.27466622621497766Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GGAGACGTCGCTGCCGTGATCGTGGTCTGAGATCGGAAGAGCACACGTC1214560.2737556308154138No Hit
CGGCTCAGCCGGAGGTAGGGTCCAGCGGCTGAGATCGGAAGAGCACACG1209500.2726151326169502No Hit
ACCGACGCGGATTACGGTGGCGGCCTAAGCCCGAGATCGTAAGAGCACA1193720.26905840108102996No Hit
AATCGAGGCAAACTCTGAATACTAGATAGATCGGAAGAGCACACGTCTG1186390.2674062564575639Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTCTGAAC1169430.2635835589386028Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGTGGAGACGTCGCTGCCGTGATCGTGGTCTAGATCGGAATAGCACACG1155640.260475363255438No Hit
CTGATGATTCATGATAACTCGACGGATCGCAAGATCGGAAGAGCACACG1094300.24664964003532752No Hit
TCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTCTGAACTCCA1041310.23470596423758283Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GACCGACGCGGATTACGGTGGCGGCCTAAGCCCGAGATCTGAAGAGCAC1025820.23121459722291846No Hit
GACCGACGCGGATTACGGTGGCGGCCTAAGCCCAGATCGGAAGAGCACA1007460.22707634684272232No Hit
AATCGAGGCAAACTCTGAATACTAGATAAGATCGGAAGAGCACACGTCT962120.216856942036726Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGATGATTCATGATAACTCGACGGAGATCGGAAGAGCACACGTCTGAA867630.19555937785237248Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCGACGCGGATTACGGTGGCGGCCTAAGCCCAGATCGGAAGAGCACAC831780.1874789706557477No Hit
TGTGGAGACGTCGCTGCCGTGATCGTGGAGATCGGAAGAGCACACGTCT828390.18671488194175723Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGACCGACGCGGATTACGGTGGCGGCCTAAGATCGGAATAGCACACGT771350.1738583567954399No Hit
TGGAGACGTCGCTGCCGTGATCGTGGTCTAGATCGGAAGATCACACGTC750250.1691025243868267No Hit
GAGACGTCGCTGCCGTGATCGTGGTCTGCAAGATCGGAAGAGCACACGT741740.16718441378031967No Hit
GAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTCTGAA718260.16189214150760697Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGGAGACGTCGCTGCCGTGATCGTGGTCTAGATCGGAATAGCACACGT703930.1586622325779659No Hit
GAGACGTCGCTGCCGTGATCGTGGTCTGAGATCGGAAGATCACACGTCT666770.15028655806118552Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TTGTGGAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACG664960.14987859329058884No Hit
GGAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTCTGA649320.14635341704079216Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGATCACACGTCTG643580.14505965030972867Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GGACCGACGCGGATTACGGTGGCGGCCTAAGATCGGAATAGCACACGTC613860.1383609138555114No Hit
CGGATTACGGTGGCGGCCTAAGCCCGAGATCGGAAGAGCACACGTCTGA611880.13791463195176476Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTCT599060.1350250693224557Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGAGACGTCGCTGCCGTGATCGTGGTCTGAGATCGGAAGAGCACACG593540.1337808894695863No Hit
GAGACGTCGCTGCCGTGATCGTGGTCTGCAGATCGGAAGAGCACACGTC573680.12930454673806696No Hit
TGGAGACGTCGCTGCCGTGATCGTGGTCTGAGATCGGAATAGCACACGT572510.12904083470403485No Hit
GTGGAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAATAGCACACGTC566700.12773129032990962No Hit
TGGAGACGTCGCTGCCGTGATCGTGGAGATCGGAAGAGCACACGTCTGA562670.12682294887935458Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACCGACGCGGATTACGGTGGCGGCCTAAGATCGGAAGAGCACACGTCT529320.11930602893493517Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATCGAGGCAAACTCTGAATACTAGATAGATCGGAAGAGCACACGTCT514690.11600850153502944Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTC511300.11524441282103896No Hit
AGACGTCGCTGCCGTGATCGTGGTCTGCAGAGATCGGAAGAGCACACGT492600.11102952817454294No Hit
TCCGATGTCGTCCAGCGGTTAGGATATCTGGAGATCGGAAGAGCACACG476170.10732628995304934No Hit
GGAGACGTCGCTGCCGTGATCGTGGTCTAGATCGGAAGATCACACGTCT467120.10528646610006595Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CGTAGGTTCGAATCCTACTTGGGGAAGATCGGAAGAGCACACGTCTGAA461900.10410990471746119Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCGACGCGGATTACGGTGGCGGCCTAAGCCCGAGATCTTAAGAGCACA450890.10162830685874881No Hit
GGACCGACGCGGATTACGGTGGCGGCCAGATCGGAAGATCACACGTCTG450090.1014479909380431Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GGACCGACGCGGATTACGGTGGCGGCCTAAAGATCGGAAGAGCACACGT447060.10076504438837021No Hit
GTGGACCGACGCGGATTACGGTGGCGGCCTAAGATCGGAAGAGCACACG445220.10035031777074707No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)