FastQCFastQC Report
Tue 25 Jul 2023
SRR10620361_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10620361_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43598909
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length49
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGT21956115.035931059651057No Hit
CCGACGCGGATTACGGTGGCGGCCTAAGCCCGAGATCGGAAGAGCACAC18704504.290130287434486No Hit
ACCGACGCGGATTACGGTGGCGGCCTAAGCCCGAGATCGGAAGAGCACA16559763.7982051339862655No Hit
TGTGGAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGT12019502.7568350391520116No Hit
GAGACGTCGCTGCCGTGATCGTGGTCTAGATCGGAAGAGCACACGTCTG9761582.238950520527933Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGAGACGTCGCTGCCGTGATCGTGGTCTAGATCGGAAGAGCACACGTC9042342.073983089806215No Hit
TGGACCGACGCGGATTACGGTGGCGGCCTAAGATCGGAAGAGCACACGT6808721.561672105143732No Hit
GAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTCTGA6723521.5421303317475215Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTCT6679551.5320452170030217Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGAGACGTCGCTGCCGTGATCGTGGTCTAGATCGGAAGAGCACACGT4539421.0411774294627418No Hit
GGACCGACGCGGATTACGGTGGCGGCCTAAGATCGGAAGAGCACACGTC4294310.9849581327826346No Hit
CGACGCGGATTACGGTGGCGGCCTAAGCCCGAGATCGGAAGAGCACACG4189320.960877254979018No Hit
TGTGGAGACGTCGCTGCCGTGATCGTGGTCTAGATCGGAAGAGCACACG4156130.9532646791689214No Hit
GAGACGTCGCTGCCGTGATCGTGGTCTGAGATCGGAAGAGCACACGTCT4147530.9512921527462992Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGAGACGTCGCTGCCGTGATCGTGGTCTGAGATCGGAAGAGCACACGT4090700.9382574229093669No Hit
GGAGACGTCGCTGCCGTGATCGTGGTCTAGATCGGAAGAGCACACGTCT3471830.7963112104479496Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGACGTCGCTGCCGTGATCGTGGTCTAGATCGGAAGAGCACACGTCTGA3450600.791441822546523Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACCGACGCGGATTACGGTGGCGGCCTAAGCCCGAGATCGGAAGAGCAC3250420.7455278296069289No Hit
CCGACGCGGATTACGGTGGCGGCCTAAGCCCAGATCGGAAGAGCACACG2903010.6658446430391183No Hit
TGTGGAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTC2710400.6216669320785068No Hit
GGAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTCTG2564950.5883060055470654Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTC2554560.5859229183922928No Hit
ATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTCTGAAC2171310.498019342639973Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGACCGACGCGGATTACGGTGGCGGCCAGATCGGAAGAGCACACGTCTG1900510.43590769668112567Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGACCGACGCGGATTACGGTGGCGGCCAGATCGGAAGAGCACACGTCT1829470.4196137109761164Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGAGACGTCGCTGCCGTGATCGTGGTCTGAGATCGGAAGAGCACACGTC1767290.40535188621348306No Hit
AGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTCTGAA1673340.38380318186402324Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTCTG1666240.38217470074767235Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGACGTCGCTGCCGTGATCGTGGTCTGAGATCGGAAGAGCACACGTCTG1562540.3583897019074491Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGATGATTCATGATAACTCGACGGAGATCGGAAGAGCACACGTCTGAA1456360.3340358814941906Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACG1315520.3017323208706897No Hit
CGTAGGTTCGAATCCTACTTGGGGAAGATCGGAAGAGCACACGTCTGAA1305390.29940886823567076Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTC1211780.27793814748896584No Hit
GACCGACGCGGATTACGGTGGCGGCCTAAGCCCAGATCGGAAGAGCACA1113280.2553458390438164No Hit
CGACGCGGATTACGGTGGCGGCCTAAGCCCAGATCGGAAGAGCACACGT1026110.235352219478703No Hit
GGACCGACGCGGATTACGGTGGCGGCCTAAAGATCGGAAGAGCACACGT1017810.23344850211733512No Hit
CGGCTCAGCCGGAGGTAGGGTCCAGCGGCTGGAGATCGGAAGAGCACAC956240.21932658911258535No Hit
TCCGATGTCGTCCAGCGGTTAGGATATCTGGAGATCGGAAGAGCACACG930550.21343423983384538No Hit
AATCGAGGCAAACTCTGAATACTAGATAAGATCGGAAGAGCACACGTCT922960.21169337058411256Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGACGCGGATTACGGTGGCGGCCTAAGCCCAGATCGGAAGAGCACAC832480.1909405577098271No Hit
CGGCTCAGCCGGAGGTAGGGTCCAGCGGCTGAGATCGGAAGAGCACACG824100.18901849126545806No Hit
TGTGGAGACGTCGCTGCCGTGATCGTGGAGATCGGAAGAGCACACGTCT739100.16952259057675045Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGCTGCCGTGATCGTGGTCTAGATCGGAAGAGCACACGTCTGAACTCC722920.16581148853977057Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCGAGGCAAACTCTGAATACTAGATAAGATCGGAAGAGCACACGTCTG718210.16473118627807865Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGACGTCGCTGCCGTGATCGTGGTCTGCAGATCGGAAGAGCACACGTC716150.1642586973907994No Hit
GGAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTCTGA714370.1638504302940241Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTCTG701670.16093751336759368Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGATGATTCATGATAACTCGACGGATCGCAAGATCGGAAGAGCACACG699770.16050172264631668No Hit
GAGACGTCGCTGCCGTGATCGTGGTCTGCAAGATCGGAAGAGCACACGT670380.15376072827877413No Hit
AATCGAGGCAAACTCTGAATACTAGATAGATCGGAAGAGCACACGTCTG659850.15134553022874953Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGTGGAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGT642930.14746469917400915No Hit
GTGGAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTCT622990.14289119023597588Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGACCGACGCGGATTACGGTGGCGGCCTAAGATCGGAAGAGCACACG615010.14106086920661248No Hit
GACCGACGCGGATTACGGTGGCGGCCTAAGATCGGAAGAGCACACGTCT556580.12765915770965738Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATCGAGGCAAACTCTGAATACTAGATAAGATCGGAAGAGCACACGTC534560.12260857261359452No Hit
TTGTGGAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACG495220.11358541104778562No Hit
TCCGTTATCGTCCAGCGGTTAGGATATCTGGAGATCGGAAGAGCACACG492440.11294778041349614No Hit
TGATGATTCATGATAACTCGACGGAGATCGGAAGAGCACACGTCTGAAC469500.10768618086292021Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGATTACGGTGGCGGCCTAAGCCCGAGATCGGAAGAGCACACGTCTGA463090.10621596058745415Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGAGACGTCGCTGCCGTGATCGTGGAGATCGGAAGAGCACACGTCTGA449320.10305762467588352Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTCTGAA438700.10062178390748264Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)