FastQCFastQC Report
Tue 18 Jul 2023
SRR10620362_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10620362_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42848978
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length49
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACGCGGATTACGGTGGCGGCCTAAGCCCGAGATCGGAAGAGCACAC21996385.133466660511717No Hit
ACCGACGCGGATTACGGTGGCGGCCTAAGCCCGAGATCGGAAGAGCACA19395524.526483688829171No Hit
GAGACGTCGCTGCCGTGATCGTGGTCTAGATCGGAAGAGCACACGTCTG12913243.0136634764077685Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGTGGAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGT12501252.917514158680751No Hit
TGTGGAGACGTCGCTGCCGTGATCGTGGTCTAGATCGGAAGAGCACACG12231282.854509155387557No Hit
CTGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGT12078472.8188466945466004No Hit
TGGAGACGTCGCTGCCGTGATCGTGGTCTAGATCGGAAGAGCACACGTC11852882.7661989977917325No Hit
GACCGACGCGGATTACGGTGGCGGCCTAAGCCCGAGATCGGAAGAGCAC10738912.506223135590305No Hit
GAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTCTGA10350102.415483515149416Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGACCGACGCGGATTACGGTGGCGGCCTAAGATCGGAAGAGCACACGT8315611.9406787251728619No Hit
TGGAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTCT7051341.6456261803957144Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACGCGGATTACGGTGGCGGCCTAAGCCCGAGATCGGAAGAGCACACG6057481.4136813251415237No Hit
AGACGTCGCTGCCGTGATCGTGGTCTAGATCGGAAGAGCACACGTCTGA5718291.3345219108843156Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGAGACGTCGCTGCCGTGATCGTGGTCTAGATCGGAAGAGCACACGT5645921.3176323598663193No Hit
GGACCGACGCGGATTACGGTGGCGGCCTAAGATCGGAAGAGCACACGTC5615951.3106380273527178No Hit
GAGACGTCGCTGCCGTGATCGTGGTCTGAGATCGGAAGAGCACACGTCT5578561.301912031600847Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGAGACGTCGCTGCCGTGATCGTGGTCTGAGATCGGAAGAGCACACGT5297881.2364075521241138No Hit
GGAGACGTCGCTGCCGTGATCGTGGTCTAGATCGGAAGAGCACACGTCT4821011.1251166830630126Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGACCGACGCGGATTACGGTGGCGGCCAGATCGGAAGAGCACACGTCTG4204130.9811505889358668Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGACGCGGATTACGGTGGCGGCCTAAGCCCAGATCGGAAGAGCACACG3240790.756328423982481No Hit
GGAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTCTG2980450.6955708488543181Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTC2767740.6459290580979551No Hit
GGAGACGTCGCTGCCGTGATCGTGGTCTGAGATCGGAAGAGCACACGTC2472000.5769099090298023No Hit
TGTGGAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTC2223790.5189832065539579No Hit
AGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTCTGAA2082180.4859345770160493Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGACGTCGCTGCCGTGATCGTGGTCTGAGATCGGAAGAGCACACGTCTG1873010.4371189436536853Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACCGACGCGGATTACGGTGGCGGCCTAAGCCCAGATCGGAAGAGCACA1475280.3442975932821548No Hit
CGGCTCAGCCGGAGGTAGGGTCCAGCGGCTGGAGATCGGAAGAGCACAC1427380.3331187969057278No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTAT1426020.33280140310464346TruSeq Adapter, Index 12 (100% over 48bp)
TGGACCGACGCGGATTACGGTGGCGGCCAGATCGGAAGAGCACACGTCT1409260.3288899912618686Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACGCGGATTACGGTGGCGGCCTAAGCCCAGATCGGAAGAGCACACGT1406130.32815951876378474No Hit
TGGAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTCTG1368210.31930983278060915Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTCTGAAC1170460.2731593738361741Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGACCGACGCGGATTACGGTGGCGGCCTAAAGATCGGAAGAGCACACGT1157300.27008812205509314No Hit
TTGTGGAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACG1118180.2609583827180195No Hit
GAGACGTCGCTGCCGTGATCGTGGTCTGCAGATCGGAAGAGCACACGTC1008590.23538251017328815No Hit
CTGATGATTCATGATAACTCGACGGAGATCGGAAGAGCACACGTCTGAA999190.23318875890108742Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGCTGCCGTGATCGTGGTCTAGATCGGAAGAGCACACGTCTGAACTCC984130.229674089309668Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAGACGTCGCTGCCGTGATCGTGGTCTGCAAGATCGGAAGAGCACACGT973220.22712793756714572No Hit
ACCGACGCGGATTACGGTGGCGGCCTAAGCCCAGATCGGAAGAGCACAC957760.2235199168577603No Hit
CGGCTCAGCCGGAGGTAGGGTCCAGCGGCTGAGATCGGAAGAGCACACG956210.2231581812756421No Hit
TCTGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACG953280.2224743843365412No Hit
TGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTC878140.2049383768266305No Hit
GTGGACCGACGCGGATTACGGTGGCGGCCTAAGATCGGAAGAGCACACG842380.19659278688047124No Hit
CGTAGGTTCGAATCCTACTTGGGGAAGATCGGAAGAGCACACGTCTGAA786080.1834536170267585Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGATTACGGTGGCGGCCTAAGCCCGAGATCGGAAGAGCACACGTCTGA742000.1731663238269067Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTCTGAA735120.1715606845978917Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACCGACGCGGATTACGGTGGCGGCCTAAGATCGGAAGAGCACACGTCT712360.166249005985627Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTCTGA690430.16113103094314174Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGATGTCGTCCAGCGGTTAGGATATCTGGAGATCGGAAGAGCACACG589620.13760421543776377No Hit
ATCGAGGCAAACTCTGAATACTAGATAAGATCGGAAGAGCACACGTCTG588380.1373148269720692Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGTGGAGACGTCGCTGCCGTGATCGTGGAGATCGGAAGAGCACACGTCT578100.13491570324034333Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGAGACGTCGCTGCCGTGATCGTGGTCTGAGATCGGAAGAGCACACG576010.13442794364897104No Hit
AATCGAGGCAAACTCTGAATACTAGATAAGATCGGAAGAGCACACGTCT548840.12808706896113134Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGATGATTCATGATAACTCGACGGATCGCAAGATCGGAAGAGCACACG533360.12447438069584764No Hit
GGAGACGTCGCTGCCGTGATCGTGGTCTGCAGATCGGAAGAGCACACGT524700.122453328991884No Hit
GACGCGGATTACGGTGGCGGCCTAAGCCCGAGATCGGAAGAGCACACGT511750.11943108654773515No Hit
GTGGAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTCT507010.11832487579983822Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGTGGAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGT476470.11119751794313507No Hit
AGACGTCGCTGCCGTGATCGTGGTCTGCAGAGATCGGAAGAGCACACGT454570.10608654423449726No Hit
TGATGATTCATGATAACTCGACGGAGATCGGAAGAGCACACGTCTGAAC452940.10570613842878587Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGACCGACGCGGATTACGGTGGCGGAGATCGGAAGAGCACACGTCTGA449600.10492665659376987Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)