FastQCFastQC Report
Wed 27 Sept 2023
SRR1062203.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1062203.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Total Bases304 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTGAATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGT1742904.35725No Hit
CATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTC1163032.907575No Hit
GCCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCTG716021.79005Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGAATGAGTGCATCCCAAACCCATGGGCGAAGATCGGAAGAGCACACGT680031.7000749999999998No Hit
GGATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTC671061.6776499999999999No Hit
GCCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCT633921.5848Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTGAATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTC614211.535525No Hit
ACGATTAATAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTG577931.444825Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGT501081.2527No Hit
ATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTCT425941.06485Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGT416671.0416750000000001No Hit
CATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCTG396090.9902249999999999Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGTC395390.988475No Hit
AGCCCGCGGACGGTGTGAGGCCGGTGTCGGCCCAGATCGGAAGAGCACAC374700.9367500000000001No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGTAGATCGGAAGAGCAC366530.916325No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGAGATCGGAAGAGCACA360500.90125No Hit
AGCCCGCGGACGGTGTGAGGCCGGTGTCGGCCAGATCGGAAGAGCACACG337040.8425999999999999No Hit
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGTAGATCGGAAGAGCACA311490.778725No Hit
ACGATTAATAGTCCTACGTGATCTGAGAGATCGGAAGAGCACACGTCTGA270830.677075Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCT262190.655475Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTC261060.6526500000000001No Hit
AGCCCGCGGACGGTGTGAGGCCGGTGTCGGCCCCAGATCGGAAGAGCACA249540.62385No Hit
CATTGAATACCCCAGCTAGGAATAATGGAAGATCGGAAGAGCACACGTCT249180.62295Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGAGATCGGAAGAGCACAC246540.61635No Hit
GTTGAACCCCGCCCGTGATGGGGACCGGGGATTAGATCGGAAGAGCACAC241240.6031No Hit
CGTTGAACCCCGCCCGTGATGGGGACCGGGGATTAGATCGGAAGAGCACA232550.5813750000000001No Hit
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGAGATCGGAAGAGCAC212460.53115No Hit
CGATTAATAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGA205500.5137499999999999Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACG203700.50925No Hit
CGACCTCAGCTCAGACGAGAAGACCCGCTGAAGATCGGAAGAGCACACGT202960.5074000000000001No Hit
TTGAATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCT195060.48765Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGAATGAGTGCATCCCAAACCCATGGGCGAAGATCGGAAGAGCACACGTC194870.487175No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGT189280.4732No Hit
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGT185880.4647No Hit
CCCATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACG182930.457325No Hit
AGGGAGTCGGGTCCAGATCCCCGAGCCCGGAGAGATCGGAAGAGCACACG173650.434125No Hit
AGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGA165100.41275Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCATTGAATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACAC154770.38692499999999996No Hit
CGACCTCAGCTCAGACGAGAAGACCCGCTGAAAGATCGGAAGAGCACACG153680.3842No Hit
CCATTGAATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACG152520.3813No Hit
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGTAGATCGGAAGAGCA151650.379125No Hit
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGT148940.37234999999999996No Hit
ATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCTGA140220.35055Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGGAGATCGGAAGAGCAC136870.342175No Hit
TTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTCTG135620.33904999999999996Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTGAACCCCGCCCGTGATGGGGACCGGGGATTGAGATCGGAAGAGCACA135270.338175No Hit
CCCATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTC135090.337725No Hit
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACG134640.3366No Hit
CCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCTG131340.32835Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCGAGAAGACGATCGAACTCGGTCGTTTAAGATCGGAAGAGCACACGTC130530.32632500000000003No Hit
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACAC120930.302325No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGTCAGATCGGAAGAGCA107730.269325No Hit
CGACCTCAGCTCAGACGAGAAGACCCGCTGAATAGATCGGAAGAGCACAC105190.262975No Hit
CGGATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGT100010.250025No Hit
CATTGAATACCCCAGCTAGGAATAATGAGATCGGAAGAGCACACGTCTGA99810.249525Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGAGAAGACGATCGAACTCGGTCGTAGATCGGAAGAGCACACGTCTG99180.24795Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGAGTCGGGTCCAGATCCCCGAGCCCGGAGCAGATCGGAAGAGCACACG96360.2409No Hit
GTTGAACCCCGCCCGTGATGGGGACCGGGGATAGATCGGAAGAGCACACG96220.24055No Hit
CGATTAATAGTCCTACGTGATCTGAGAGATCGGAAGAGCACACGTCTGAA94220.23554999999999998Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGTCAGATCGGAAGAGCAC94060.23515No Hit
GCGCCCCATTGAATACCCCAGCTAGGAATAAGATCGGAAGAGCACACGTC91550.228875No Hit
GAGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACG91100.22775No Hit
ATTGAATACCCCAGCTAGGAATAATGGAAGATCGGAAGAGCACACGTCTG90650.226625Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGAGAAGACGATCGAACTCGGTCGTTTAAGATCGGAAGAGCACACGT89450.223625No Hit
AGCCCGCGGACGGTGTGAGGCCGGTGTCGAGATCGGAAGAGCACACGTCT87790.219475Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCTTGCTCTCCGGGGCCGCGGGTGAGTGAGATCGGAAGAGCACACGTCT87690.21922500000000003Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGTC87530.21882500000000002No Hit
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGGAGATCGGAAGAGCACA85080.2127No Hit
CATCCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACG81700.20425000000000001No Hit
GGGAGTCGGGTCCAGATCCCCGAGCCCGGAGAGATCGGAAGAGCACACGT81030.20257500000000003No Hit
GGGAGTCGGGTCCAGATCCCCGAGCCCGGAGCGAGATCGGAAGAGCACAC80470.20117500000000002No Hit
ACCCGGGGACGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA79780.19945000000000002Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAACGATGCCGACCCGCGATCCGGCGGCGAGATCGGAAGAGCACACGTCT79470.198675Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTG76220.19055Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCCGGGGCCGCGGGTGAGTGTCCTCCGCGCAGATCGGAAGAGCACACGT74970.187425No Hit
CCCATTGAATACCCCAGCTAGGAATAATGAGATCGGAAGAGCACACGTCT72630.18157500000000001Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGGGAGTCGGGTCCAGATCCCCGAGCCCGGAGAGATCGGAAGAGCACAC69630.17407499999999998No Hit
GAGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACAC69420.17355No Hit
CGTTGAACCCCGCCCGTGATGGGGACCGGGGATAGATCGGAAGAGCACAC68980.17245No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG68170.170425TruSeq Adapter, Index 1 (100% over 49bp)
CCCCATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGT66140.16535No Hit
AGGGAGTCGGGTCCAGATCCCCGAGCCCGGAGCAGATCGGAAGAGCACAC64970.162425No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGTCT64350.160875Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCTTGCTCTCCGGGGCCGCGGGTGAGTAGATCGGAAGAGCACACGTCTG64110.160275Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGTC63030.157575No Hit
CGTTGAACCCCGCCCGTGATGGGGACCGGGGATTGAGATCGGAAGAGCAC63030.157575No Hit
CCATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCT62230.15557500000000002Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCCGGGGCCGCGGGTGAGTGTCCTCCGCGCCAGATCGGAAGAGCACACG59310.148275No Hit
GAGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACA58390.145975No Hit
TTGGAATGAGTGCATCCCAAACCCATGGGCGAAGATCGGAAGAGCACACG55010.137525No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGAGATCGGAAGAGCACAC54860.13715000000000002No Hit
CGACCTCAGCTCAGACGAGAAGACCCGCTGAATTAGATCGGAAGAGCACA54630.136575No Hit
AGCCCGCGGACGGTGTGAGGCCGGTGTCGGCCCCCAGATCGGAAGAGCAC53620.13405No Hit
GCCGAGAAGACGATCGAACTCGGTCGTAGATCGGAAGAGCACACGTCTGA53070.13267500000000002Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGTCT50950.127375Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCCCATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACAC50170.12542499999999998No Hit
GTGAAGGGCAGGGCGCCCTGGAACGGGTTAGATCGGAAGAGCACACGTCT48130.12032499999999999Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTAATAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGAAC47840.1196Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGGAGATCGGAAGAGCA47550.118875No Hit
AACGATTAATAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCT46970.117425Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGAGTCGGGTCCAGATCCCCGAGCCCGGAGCGAGATCGGAAGAGCACA46740.11685000000000001No Hit
TTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCTGAA45710.11427500000000002Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGGAGCGCGCGCGATGGCACCCGAAAAGATCGGAAGAGCACACGTCTGA43360.1084Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGAGATCGGAAGAGCACACG42090.105225No Hit
CCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCTGA41600.104Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GATTAATAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGAA41060.10265Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGGATAACTGTGGCAATTCCAGAGCTAATACAGATCGGAAGAGCACACG41010.10252499999999999No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)