FastQCFastQC Report
Wed 27 Sept 2023
SRR1062205.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1062205.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Total Bases304 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTGAATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGT1752964.3824000000000005No Hit
CATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTC1179702.94925No Hit
GCCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCTG703721.7593Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGAATGAGTGCATCCCAAACCCATGGGCGAAGATCGGAAGAGCACACGT681431.7035749999999998No Hit
GGATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTC662781.6569500000000001No Hit
GCCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCT631841.5796000000000001Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTGAATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTC624261.56065No Hit
ACGATTAATAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTG581211.453025Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGT493201.233No Hit
ATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTCT426561.0664Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGT421491.053725No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGTAGATCGGAAGAGCAC411171.027925No Hit
CATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCTG401381.00345Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGTC396170.990425No Hit
AGCCCGCGGACGGTGTGAGGCCGGTGTCGGCCCAGATCGGAAGAGCACAC363120.9077999999999999No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGAGATCGGAAGAGCACA360340.9008499999999999No Hit
AGCCCGCGGACGGTGTGAGGCCGGTGTCGGCCAGATCGGAAGAGCACACG325620.81405No Hit
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGTAGATCGGAAGAGCACA324360.8109No Hit
ACGATTAATAGTCCTACGTGATCTGAGAGATCGGAAGAGCACACGTCTGA271410.6785249999999999Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCT255380.6384500000000001Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTC254380.63595No Hit
CATTGAATACCCCAGCTAGGAATAATGGAAGATCGGAAGAGCACACGTCT252650.631625Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCCGCGGACGGTGTGAGGCCGGTGTCGGCCCCAGATCGGAAGAGCACA243370.608425No Hit
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGAGATCGGAAGAGCACAC241790.604475No Hit
GTTGAACCCCGCCCGTGATGGGGACCGGGGATTAGATCGGAAGAGCACAC239180.59795No Hit
CGTTGAACCCCGCCCGTGATGGGGACCGGGGATTAGATCGGAAGAGCACA228350.570875No Hit
CGATTAATAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGA207860.51965Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGAGATCGGAAGAGCAC204840.5121No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACG203180.50795No Hit
CGACCTCAGCTCAGACGAGAAGACCCGCTGAAGATCGGAAGAGCACACGT200030.500075No Hit
TTGAATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCT196340.49084999999999995Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGT195310.488275No Hit
GGAATGAGTGCATCCCAAACCCATGGGCGAAGATCGGAAGAGCACACGTC194960.4874No Hit
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGT185760.4644No Hit
CCCATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACG184850.462125No Hit
AGGGAGTCGGGTCCAGATCCCCGAGCCCGGAGAGATCGGAAGAGCACACG170800.42700000000000005No Hit
AGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGA162880.4072Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGTAGATCGGAAGAGCA159650.399125No Hit
CCCATTGAATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACAC154360.3859No Hit
CCATTGAATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACG149940.37485No Hit
CGACCTCAGCTCAGACGAGAAGACCCGCTGAAAGATCGGAAGAGCACACG148830.372075No Hit
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGT144690.36172499999999996No Hit
ATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCTGA144570.361425Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTC136620.34155No Hit
TTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTCTG136580.34145Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTGAACCCCGCCCGTGATGGGGACCGGGGATTGAGATCGGAAGAGCACA135360.3384No Hit
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACG130370.325925No Hit
CCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCTG128980.32245Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCGAGAAGACGATCGAACTCGGTCGTTTAAGATCGGAAGAGCACACGTC127230.318075No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGTCAGATCGGAAGAGCA118720.2968No Hit
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACAC118630.29657500000000003No Hit
CGACCTCAGCTCAGACGAGAAGACCCGCTGAATAGATCGGAAGAGCACAC103990.259975No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGGAGATCGGAAGAGCAC102890.257225No Hit
CATTGAATACCCCAGCTAGGAATAATGAGATCGGAAGAGCACACGTCTGA99510.248775Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGAGAAGACGATCGAACTCGGTCGTAGATCGGAAGAGCACACGTCTG98680.2467Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGT98440.2461No Hit
GTTGAACCCCGCCCGTGATGGGGACCGGGGATAGATCGGAAGAGCACACG95800.2395No Hit
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGTCAGATCGGAAGAGCAC95610.239025No Hit
CGATTAATAGTCCTACGTGATCTGAGAGATCGGAAGAGCACACGTCTGAA94620.23655Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGAGTCGGGTCCAGATCCCCGAGCCCGGAGCAGATCGGAAGAGCACACG93610.23402499999999998No Hit
ATTGAATACCCCAGCTAGGAATAATGGAAGATCGGAAGAGCACACGTCTG89880.22469999999999998Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGAGAAGACGATCGAACTCGGTCGTTTAAGATCGGAAGAGCACACGT88460.22114999999999999No Hit
GAGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACG88330.22082500000000002No Hit
GCGCCCCATTGAATACCCCAGCTAGGAATAAGATCGGAAGAGCACACGTC88030.220075No Hit
CGCTTGCTCTCCGGGGCCGCGGGTGAGTGAGATCGGAAGAGCACACGTCT85110.212775Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGTC84610.211525No Hit
GGGAGTCGGGTCCAGATCCCCGAGCCCGGAGAGATCGGAAGAGCACACGT83550.20887499999999998No Hit
AGCCCGCGGACGGTGTGAGGCCGGTGTCGAGATCGGAAGAGCACACGTCT83310.208275Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACGATGCCGACCCGCGATCCGGCGGCGAGATCGGAAGAGCACACGTCT80130.20032499999999998Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CATCCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACG79540.19884999999999997No Hit
GGGAGTCGGGTCCAGATCCCCGAGCCCGGAGCGAGATCGGAAGAGCACAC78840.1971No Hit
ACCCGGGGACGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA78130.19532500000000003Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTG74890.187225Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCATTGAATACCCCAGCTAGGAATAATGAGATCGGAAGAGCACACGTCT72610.181525Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCCGGGGCCGCGGGTGAGTGTCCTCCGCGCAGATCGGAAGAGCACACGT70690.176725No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG69710.17427499999999999TruSeq Adapter, Index 1 (100% over 49bp)
GAGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACAC69300.17325000000000002No Hit
AAGGGAGTCGGGTCCAGATCCCCGAGCCCGGAGAGATCGGAAGAGCACAC68920.17229999999999998No Hit
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGGAGATCGGAAGAGCACA68750.171875No Hit
CGTTGAACCCCGCCCGTGATGGGGACCGGGGATAGATCGGAAGAGCACAC68060.17015000000000002No Hit
CCCCATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGT65300.16325No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGTCT63670.159175Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTTGAACCCCGCCCGTGATGGGGACCGGGGATTGAGATCGGAAGAGCAC62960.1574No Hit
CGCTTGCTCTCCGGGGCCGCGGGTGAGTAGATCGGAAGAGCACACGTCTG62730.156825Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGAGTCGGGTCCAGATCCCCGAGCCCGGAGCAGATCGGAAGAGCACAC62470.156175No Hit
TCCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGTC61740.15435No Hit
CCATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCT61350.15337499999999998Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCCGGGGCCGCGGGTGAGTGTCCTCCGCGCCAGATCGGAAGAGCACACG58480.1462No Hit
GAGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACA56130.140325No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGAGATCGGAAGAGCACAC54280.1357No Hit
CGACCTCAGCTCAGACGAGAAGACCCGCTGAATTAGATCGGAAGAGCACA53580.13394999999999999No Hit
GCCGAGAAGACGATCGAACTCGGTCGTAGATCGGAAGAGCACACGTCTGA52590.131475Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGGAATGAGTGCATCCCAAACCCATGGGCGAAGATCGGAAGAGCACACG52220.13055No Hit
AGCCCGCGGACGGTGTGAGGCCGGTGTCGGCCCCCAGATCGGAAGAGCAC52220.13055No Hit
CCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGTCT50170.12542499999999998Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCCCATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACAC49190.122975No Hit
GTGAAGGGCAGGGCGCCCTGGAACGGGTTAGATCGGAAGAGCACACGTCT49150.122875Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTAATAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGAAC47700.11925Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AACGATTAATAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCT47220.11804999999999999Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGAGTCGGGTCCAGATCCCCGAGCCCGGAGCGAGATCGGAAGAGCACA44950.112375No Hit
TTGGAGCGCGCGCGATGGCACCCGAAAAGATCGGAAGAGCACACGTCTGA44230.11057499999999999Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCTGAA43590.108975Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGGATAACTGTGGCAATTCCAGAGCTAATACAGATCGGAAGAGCACACG40820.10204999999999999No Hit
TCTCTCGGCGCGGGCCGGGGGGCCAAAGTCAGATCGGAAGAGCACACGTC40720.1018No Hit
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGGAGATCGGAAGAGCA40690.101725No Hit
GATTAATAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGAA40200.1005Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAGCGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGT40030.100075No Hit
CCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCTGA40010.100025Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)