FastQCFastQC Report
Wed 27 Sept 2023
SRR1062207.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1062207.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Total Bases304 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCTG1374433.436075Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCT1140472.851175Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTC919622.2990500000000003No Hit
TGGAATGAGTGCATCCCAAACCCATGGGCGAAGATCGGAAGAGCACACGT792061.98015No Hit
ATCCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGT597751.494375No Hit
ACGATTAATAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTG592841.4821Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTC585001.4625000000000001No Hit
CGCCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCT465971.164925Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CATTGAATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGT431691.0792249999999999No Hit
CGCCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTC423951.0598750000000001No Hit
CCATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGT415561.0389000000000002No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGTC352060.88015No Hit
AGCCCGCGGACGGTGTGAGGCCGGTGTCGGCCAGATCGGAAGAGCACACG317200.7929999999999999No Hit
CATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCTG295940.73985Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCCGCGGACGGTGTGAGGCCGGTGTCGGCCCAGATCGGAAGAGCACAC276920.6923No Hit
ACGATTAATAGTCCTACGTGATCTGAGAGATCGGAAGAGCACACGTCTGA275020.68755Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCTG259210.648025Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGTAGATCGGAAGAGCACA243240.6081No Hit
CCCATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACG237680.5942000000000001No Hit
AGCCCGCGGACGGTGTGAGGCCGGTGTCGAGATCGGAAGAGCACACGTCT232840.5821Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCCGGGGACGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA228880.5721999999999999Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGAATGAGTGCATCCCAAACCCATGGGCGAAGATCGGAAGAGCACACGTC227840.5696No Hit
AGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGA227460.56865Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACCTCAGCTCAGACGAGAAGACCCGCTGAAGATCGGAAGAGCACACGT221480.5537000000000001No Hit
CCCATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTC203510.508775No Hit
CATTGAATACCCCAGCTAGGAATAATGGAAGATCGGAAGAGCACACGTCT201590.503975Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGAGAAGACGATCGAACTCGGTCGTTTAAGATCGGAAGAGCACACGTC194370.485925No Hit
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGAGATCGGAAGAGCACAC180780.45195No Hit
AAACGATGCCGACCCGCGATCCGGCGGCGAGATCGGAAGAGCACACGTCT173550.43387499999999996Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTCT167770.419425Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGTAGATCGGAAGAGCAC164110.41027499999999995No Hit
GAGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTG156100.39025000000000004Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGAGATCGGAAGAGCACA144210.360525No Hit
CGATTAATAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGA143730.359325Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGGAGTCGGGTCCAGATCCCCGAGCCCGGAGAGATCGGAAGAGCACACG137530.343825No Hit
CCATTGAATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACG136390.34097500000000003No Hit
ATTGAATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTC134310.335775No Hit
GCCGAGAAGACGATCGAACTCGGTCGTAGATCGGAAGAGCACACGTCTGA130290.32572500000000004Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCTTGCTCTCCGGGGCCGCGGGTGAGTGAGATCGGAAGAGCACACGTCT126210.315525Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGTC123540.30885No Hit
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGT112520.2813No Hit
GGGAGTCGGGTCCAGATCCCCGAGCCCGGAGCAGATCGGAAGAGCACACG110560.2764No Hit
CGGATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGT108590.271475No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGT107170.267925No Hit
GGGAGTCGGGTCCAGATCCCCGAGCCCGGAGCGAGATCGGAAGAGCACAC102510.256275No Hit
GTTGAACCCCGCCCGTGATGGGGACCGGGGATTAGATCGGAAGAGCACAC100710.251775No Hit
GTTGAACCCCGCCCGTGATGGGGACCGGGGATTGAGATCGGAAGAGCACA91940.22985000000000003No Hit
CCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCTGA91800.2295Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACCTCAGCTCAGACGAGAAGACCCGCTGAAAGATCGGAAGAGCACACG91100.22775No Hit
CCATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCT90640.22659999999999997Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGAGTCGGGTCCAGATCCCCGAGCCCGGAGAGATCGGAAGAGCACACGT90050.22512500000000002No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGTCT86410.216025Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCCGCGGACGGTGTGAGGCCGGTGTCGGCCCCAGATCGGAAGAGCACA85140.21285No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACG80900.20224999999999999No Hit
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGTC78260.19565No Hit
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGGAGATCGGAAGAGCACA78050.19512500000000002No Hit
ATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCTGA78030.195075Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATTCCAGAGCTAATACATGCAGATCGGAAGAGCACACGTCTGAACTCCA76820.19205Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGT72980.18245No Hit
CGCCGAGAAGACGATCGAACTCGGTCGTAGATCGGAAGAGCACACGTCTG72170.180425Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CATTGAATACCCCAGCTAGGAATAATGAGATCGGAAGAGCACACGTCTGA71730.17932499999999998Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGAGAAGACGATCGAACTCGGTCGTTTAAGATCGGAAGAGCACACGT70820.17704999999999999No Hit
CCCCATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGT67710.169275No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGGAGATCGGAAGAGCAC67220.16805No Hit
AACGATGCCGACCCGCGATCCGGCGGCGAGATCGGAAGAGCACACGTCTG66550.166375Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGATAACTGTGGCAATTCCAGAGCTAATACAGATCGGAAGAGCACACG64720.1618No Hit
CCCATTGAATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACAC63000.1575No Hit
GCGCCCCATTGAATACCCCAGCTAGGAATAAGATCGGAAGAGCACACGTC62530.156325No Hit
CGACCTTTCGAACGCACATTGCGGCCCCGGGAGATCGGAAGAGCACACGT61110.152775No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT61080.1527No Hit
AGCCCGCGGACGGTGTGAGGCCGGTGTCGGCAGATCGGAAGAGCACACGT60330.15082500000000001No Hit
CTCCGGGGCCGCGGGTGAGTGTCCTCCGCGCAGATCGGAAGAGCACACGT59710.149275No Hit
CGATTAATAGTCCTACGTGATCTGAGAGATCGGAAGAGCACACGTCTGAA59510.148775Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGGAATGAGTGCATCCCAAACCCATGGGCGAAGATCGGAAGAGCACACG59210.148025No Hit
CCCATTGAATACCCCAGCTAGGAATAATGAGATCGGAAGAGCACACGTCT58100.14525Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGTCT57560.1439Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG55540.13885TruSeq Adapter, Index 2 (100% over 49bp)
CATCCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACG55220.13805No Hit
AACGATTAATAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCT52800.132Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTCTG52710.131775Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCTTGCTCTCCGGGGCCGCGGGTGAGTAGATCGGAAGAGCACACGTCTG51930.129825Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTGAATACCCCAGCTAGGAATAATGGAAGATCGGAAGAGCACACGTCTG51220.12805Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGGGAGTCGGGTCCAGATCCCCGAGCCCGGAGAGATCGGAAGAGCACAC50360.12589999999999998No Hit
AAGGAGTCTAACACGTGCGCGAGTAGATCGGAAGAGCACACGTCTGAACT48970.122425Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCCCTGTCCGCGGCTGGAGCGTCGCAGACAGATCGGAAGAGCACACGTCT48880.1222Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACG48070.12017499999999999No Hit
GATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTCT47580.11895Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAAACGATGCCGACCCGCGATCCGGCGGCGAGATCGGAAGAGCACACGTC46880.11720000000000001No Hit
GCGCCCCATTGAATACCCCAGCTAGGAAGATCGGAAGAGCACACGTCTGA45450.113625Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGAGATCGGAAGAGCAC45300.11325No Hit
TTGAATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCT43760.1094Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACG42160.10540000000000001No Hit
AGCCCGCGGACGGTGTGAGGCCGGTGTCAGATCGGAAGAGCACACGTCTG42110.10527500000000001Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCCGGGGCCGCGGGTGAGTGTCCTCCGCGAGATCGGAAGAGCACACGTC41590.10397500000000001No Hit
CCCATTGAATACCCCAGCTAGGAATAATGGAAGATCGGAAGAGCACACGT40930.10232500000000001No Hit
GCCGAGAAGACGATCGAACTCGGTCGTTTAGAGATCGGAAGAGCACACGT40510.101275No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)