FastQCFastQC Report
Wed 27 Sept 2023
SRR1062215.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1062215.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3993526
Total Bases303.5 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCTG1343793.3649211248405546Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCT1121232.8076191315644374Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTC910872.2808665825638794No Hit
TGGAATGAGTGCATCCCAAACCCATGGGCGAAGATCGGAAGAGCACACGT767151.920984112786545No Hit
CATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTC591851.4820236552860804No Hit
ACGATTAATAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTG585071.4650461772378596Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGT576581.443786768885441No Hit
CGCCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCT444301.1125506632484676Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CATTGAATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGT432561.083153083265265No Hit
CCATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGT415081.039382240155692No Hit
CGCCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTC406931.0189742097584942No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGTC346610.8679297442911352No Hit
CATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCTG297790.7456818861327058Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCCGCGGACGGTGTGAGGCCGGTGTCGGCCAGATCGGAAGAGCACACG297100.74395408969417No Hit
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGTAGATCGGAAGAGCACA275930.690943291717645No Hit
ACGATTAATAGTCCTACGTGATCTGAGAGATCGGAAGAGCACACGTCTGA270930.6784230276702843Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCCGCGGACGGTGTGAGGCCGGTGTCGGCCCAGATCGGAAGAGCACAC259530.6498768256423021No Hit
CCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCTG255710.6403113439101186Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACG234910.5882270454730982No Hit
GGAATGAGTGCATCCCAAACCCATGGGCGAAGATCGGAAGAGCACACGTC225210.5639377332212185No Hit
AGCCCGCGGACGGTGTGAGGCCGGTGTCGAGATCGGAAGAGCACACGTCT218500.5471355388696605Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGA215110.5386467998455501Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGTAGATCGGAAGAGCAC213940.5357170580584676No Hit
CGACCTCAGCTCAGACGAGAAGACCCGCTGAAGATCGGAAGAGCACACGT211500.5296071692033557No Hit
CATTGAATACCCCAGCTAGGAATAATGGAAGATCGGAAGAGCACACGTCT206150.5162104866726797Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTC201030.5033897362881824No Hit
ACCCGGGGACGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA192940.4831319490595529Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGAGAAGACGATCGAACTCGGTCGTTTAAGATCGGAAGAGCACACGTC190860.47792351921585086No Hit
AAACGATGCCGACCCGCGATCCGGCGGCGAGATCGGAAGAGCACACGTCT171710.4299709079144596Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTCT168430.421757614699391Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGAGATCGGAAGAGCACAC168040.42078103410369677No Hit
GAGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTG148900.37285346333040026Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGAGATCGGAAGAGCACA146950.3679705603519296No Hit
CGATTAATAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGA142480.35677744429358915Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCATTGAATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACG136160.3409518305377253No Hit
ATTGAATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTC135040.3381472913911165No Hit
AGGGAGTCGGGTCCAGATCCCCGAGCCCGGAGAGATCGGAAGAGCACACG132300.3312861866931629No Hit
GCCGAGAAGACGATCGAACTCGGTCGTAGATCGGAAGAGCACACGTCTGA125960.3154104918811096Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGTC122540.3068466312727149No Hit
CGCTTGCTCTCCGGGGCCGCGGGTGAGTGAGATCGGAAGAGCACACGTCT121240.30359136262040115Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGT112770.28238203532417216No Hit
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGT110460.2765976733342915No Hit
GGGAGTCGGGTCCAGATCCCCGAGCCCGGAGCAGATCGGAAGAGCACACG105210.26345139608456286No Hit
CGGATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGT102400.2564150076899462No Hit
GTTGAACCCCGCCCGTGATGGGGACCGGGGATTAGATCGGAAGAGCACAC99240.24850220081201424No Hit
GGGAGTCGGGTCCAGATCCCCGAGCCCGGAGCGAGATCGGAAGAGCACAC99120.2482017144748776No Hit
GTTGAACCCCGCCCGTGATGGGGACCGGGGATTGAGATCGGAAGAGCACA94690.23710876052891605No Hit
CCATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCT89970.22528963126820759Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACCTCAGCTCAGACGAGAAGACCCGCTGAAAGATCGGAAGAGCACACG89010.22288574057111435No Hit
CCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCTGA87640.21945518822213753Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGAGTCGGGTCCAGATCCCCGAGCCCGGAGAGATCGGAAGAGCACACGT85980.2152984605584138No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGTCT83670.20951409856853317Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCCGCGGACGGTGTGAGGCCGGTGTCGGCCCCAGATCGGAAGAGCACA81490.20405526344388392No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACG80620.2018767374996432No Hit
ATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCTGA77990.19529107861073147Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATTCCAGAGCTAATACATGCAGATCGGAAGAGCACACGTCTGAACTCCA76930.19263678263269102Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGTC72900.18254544981051832No Hit
CATTGAATACCCCAGCTAGGAATAATGAGATCGGAAGAGCACACGTCTGA72360.18119326129340335Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGAGAAGACGATCGAACTCGGTCGTAGATCGGAAGAGCACACGTCTG67940.17012534787553657Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGT67290.16849771354937967No Hit
AACGATGCCGACCCGCGATCCGGCGGCGAGATCGGAAGAGCACACGTCTG66670.16694520080750697Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGAGAAGACGATCGAACTCGGTCGTTTAAGATCGGAAGAGCACACGT65970.16519236384087646No Hit
TTGGATAACTGTGGCAATTCCAGAGCTAATACAGATCGGAAGAGCACACG64420.16131108198619465No Hit
CCCCATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGT63200.15825613755863865No Hit
CCCATTGAATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACAC61520.1540493288387255No Hit
CGACCTTTCGAACGCACATTGCGGCCCCGGGAGATCGGAAGAGCACACGT59890.1499677227592859No Hit
CGATTAATAGTCCTACGTGATCTGAGAGATCGGAAGAGCACACGTCTGAA59760.14964219589405453Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT58950.1476139131183821No Hit
CCCATTGAATACCCCAGCTAGGAATAATGAGATCGGAAGAGCACACGTCT58220.14578595456746746Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGCCCCATTGAATACCCCAGCTAGGAATAAGATCGGAAGAGCACACGTC57960.14513490083700467No Hit
TTGGAATGAGTGCATCCCAAACCCATGGGCGAAGATCGGAAGAGCACACG56460.1413788216227965No Hit
AGCCCGCGGACGGTGTGAGGCCGGTGTCGGCAGATCGGAAGAGCACACGT55910.14000159257758682No Hit
CTCCGGGGCCGCGGGTGAGTGTCCTCCGCGCAGATCGGAAGAGCACACGT55680.13942566043140822No Hit
CCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGTCT54940.13757266135239887Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACGATTAATAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCT54200.13571966227338947Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG53580.13416714953151676TruSeq Adapter, Index 2 (100% over 49bp)
TTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTCTG53400.13371642002581177Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CATCCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACG52080.13041107031730856No Hit
ATTGAATACCCCAGCTAGGAATAATGGAAGATCGGAAGAGCACACGTCTG51440.1288084765192464Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCTTGCTCTCCGGGGCCGCGGGTGAGTAGATCGGAAGAGCACACGTCTG50080.12540296469836432Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGGGAGTCGGGTCCAGATCCCCGAGCCCGGAGAGATCGGAAGAGCACAC49140.1230491550574605No Hit
AAGGAGTCTAACACGTGCGCGAGTAGATCGGAAGAGCACACGTCTGAACT47600.11919291373087343Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAAACGATGCCGACCCGCGATCCGGCGGCGAGATCGGAAGAGCACACGTC46290.11591260455046493No Hit
CCCCTGTCCGCGGCTGGAGCGTCGCAGACAGATCGGAAGAGCACACGTCT46020.11523651029190746Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTCT45070.11285766012290893Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACG45030.11275749801053005No Hit
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGAGATCGGAAGAGCAC44050.11030352625724736No Hit
GCCGAGAAGACGATCGAACTCGGTCGTTTAGAGATCGGAAGAGCACACGT42580.10662256862732332No Hit
TTGAATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCT42560.10657248757113388Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGCCCCATTGAATACCCCAGCTAGGAAGATCGGAAGAGCACACGTCTGA42210.10569606908781863Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTAAATGACTCGTAGACGACCTGATTAGATCGGAAGAGCACACGTCTGAA40310.1009383687498216Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTTGAACCCCGCCCGTGATGGGGACCGGGGATAGATCGGAAGAGCACACG40260.10081316610934798No Hit
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACG40150.10053772030030605No Hit
CCCATTGAATACCCCAGCTAGGAATAATGGAAGATCGGAAGAGCACACGT40040.1002622744912641No Hit
CTCCGGGGCCGCGGGTGAGTGTCCTCCGCGAGATCGGAAGAGCACACGTC39980.10011203132269579No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)