FastQCFastQC Report
Wed 27 Sept 2023
SRR1062217.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1062217.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2318708
Total Bases176.2 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGT26645411.491485775699225No Hit
AGCCCGCGGACGGTGTGAGGCCGGTGTCGGCCCCAGATCGGAAGAGCACA543022.3419076485698067No Hit
GCCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCTG523342.2570327958501024Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGTC504702.1766431995749356No Hit
AGCCCGCGGACGGTGTGAGGCCGGTGTCGGCCCAGATCGGAAGAGCACAC476132.0534280297476006No Hit
AGCCCGCGGACGGTGTGAGGCCGGTGTCGGCCCCCAGATCGGAAGAGCAC442241.9072690481078256No Hit
CATCCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACG428031.8459849191877546No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGT350811.5129546281808661No Hit
GGATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTC344731.4867331289666486No Hit
AGCCCGCGGACGGTGTGAGGCCGGTGTCGGCCAGATCGGAAGAGCACACG341331.472069790590277No Hit
GCCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCT333391.437826582734868Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACG273731.1805281216953578No Hit
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGT269621.1628027332462734No Hit
GTTGAACCCCGCCCGTGATGGGGACCGGGGATTAGATCGGAAGAGCACAC231320.9976245391830278No Hit
CCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGTCT190190.8202412722947434Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGTC179400.7737067366826699No Hit
GTTGAACCCCGCCCGTGATGGGGACCGGGGATTGAGATCGGAAGAGCACA177710.7664181949602968No Hit
TCCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGTC172030.7419217943785936No Hit
AGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGA171350.7389891267033193Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGTAGATCGGAAGAGCAC165770.7149240007797446No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGAGATCGGAAGAGCACA159130.686287363480007No Hit
CGTTGAACCCCGCCCGTGATGGGGACCGGGGATTGAGATCGGAAGAGCAC152060.6557962451503164No Hit
CATTGAATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGT148590.6408310145132547No Hit
CGCTTGCTCTCCGGGGCCGCGGGTGAGTGAGATCGGAAGAGCACACGTCT139650.60227506007656Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGTCT125280.5403008917034832Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACGATGCCGACCCGCGATCCGGCGGCGAGATCGGAAGAGCACACGTCTG124210.5356862528615073Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGT122470.5281820738100701No Hit
CGCTTGCTCTCCGGGGCCGCGGGTGAGTAGATCGGAAGAGCACACGTCTG121220.5227911405834629Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGTAGATCGGAAGAGCACA108030.4659060131763033No Hit
GTTGAACCCCGCCCGTGATGGGGACCGGGGATAGATCGGAAGAGCACACG107590.46400840468053767No Hit
CGACCTCAGCTCAGACGAGAAGACCCGCTGAAGATCGGAAGAGCACACGT106150.457798049603486No Hit
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACG104600.45111329240249315No Hit
GAGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACG91840.39608264602528653No Hit
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACAC91290.3937106354055793No Hit
AGCCCGCGGACGGTGTGAGGCCGGTGTCGAGATCGGAAGAGCACACGTCT88500.381678072443792Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGAGATCGGAAGAGCACAC86730.37404451099491615No Hit
CCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCTG85690.3695592545503789Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTTGAACCCCGCCCGTGATGGGGACCGGGGATTAGATCGGAAGAGCACA85030.3667128418067303No Hit
CATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTC76970.33195210436156686No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG76410.32953696627604684TruSeq Adapter, Index 3 (100% over 49bp)
CGCCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCT75350.3249654548998839Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGAGAAGACGATCGAACTCGGTCGTAGATCGGAAGAGCACACGTCTGA75230.3244479253101296Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCA70040.30206477055325637No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGGAGATCGGAAGAGCAC69320.29895959301473063No Hit
GAGCGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGT68260.29438808163856767No Hit
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAGATCGGAAGAGCAC67700.29197294355304765No Hit
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGTAGATCGGAAGAGCA65020.28041478271520176No Hit
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGAGATCGGAAGAGCAC63890.2755413790783488No Hit
CGCCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTC62770.2707111029073087No Hit
GAGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTG61240.2641126006379415Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGAATGAGTGCATCCCAAACCCATGGGCGAAGATCGGAAGAGCACACGT56030.24164319094944253No Hit
CGGATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGT53910.23250016819711666No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGAGATCGGAAGAGCACAC48450.20895257186329627No Hit
AGCCCGCGGACGGTGTGAGGCCGGTGTCGGAGATCGGAAGAGCACACGTC47990.2069687084359048No Hit
ACCCGGGGACGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA46890.20222468719649045Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACG46760.20166403014092332No Hit
CGCCGAGAAGACGATCGAACTCGGTCGTAGATCGGAAGAGCACACGTCTG46530.20067209842722758Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACAC45660.19692000890150896No Hit
AGCCCGCGGACGGTGTGAGGCCGGTGTCGGCCCCCGAGATCGGAAGAGCA45520.19631622438012897No Hit
CTCCGGGGCCGCGGGTGAGTGTCCTCCGCGCAGATCGGAAGAGCACACGT45150.19472050814505318No Hit
CGACCTCAGCTCAGACGAGAAGACCCGCTGAAAGATCGGAAGAGCACACG44820.1932973017732289No Hit
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCAGATCGGAAGAGCA44240.19079590875608313No Hit
GCTTGCTCTCCGGGGCCGCGGGTGAGTGAGATCGGAAGAGCACACGTCTG43400.18717320162780307Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTGAATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTC42820.1846718086106573No Hit
AAGCTTGGGCTGCAGGTCGACCCGTGCAGATCGGAAGAGCACACGTCTGA42210.18204103319607298Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCTGA41200.17768515914897434Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGGAGATCGGAAGAGCACA40050.17272550058049568No Hit
CGCTTGCTCTCCGGGGCCGCGGGTGAGAGATCGGAAGAGCACACGTCTGA38600.16647201803763131Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGAGAAGACGATCGAACTCGGTCGTTTAAGATCGGAAGAGCACACGTC37710.16263367358028696No Hit
ATGCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGT36330.15668208329811256No Hit
ATCGACCTTTCGAACGCACATTGCGGCCCCGGGAGATCGGAAGAGCACAC35360.15249871911426535No Hit
CGCTTGCTCTCCGGGGCCGCGGGTGAGTGTCCTAGATCGGAAGAGCACAC35160.1516361697980082No Hit
CATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCTG34880.15042860075524817Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAACGATGCCGACCCGCGATCCGGCGGCGAGATCGGAAGAGCACACGTCT33720.14542581472095667Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACA32470.14003488149434945No Hit
ACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGTC32320.13938796950715657No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGTCAGATCGGAAGAGCA32190.1388273124515894No Hit
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGTC31910.1376197434088294No Hit
CCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGTCTG31130.13425580107542648Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTCGGCGCGGGCCGGGGGGCCAAAGTCAGATCGGAAGAGCACACGTCTG30820.1329188496352279Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCCGGGGCCGCGGGTGAGTGTCCTCCGCGCCAGATCGGAAGAGCACACG30380.13102124113946215No Hit
ACCCGGAGATGCCGGCGGGTGCCCCGGGAAAGAGAGATCGGAAGAGCACA27870.12019624722043483No Hit
GAACGGAAGGACGGACGGCGCCGGACGCGCAGATCGGAAGAGCACACGTC27550.11881616831442338No Hit
ACCCGGAGATGCCGGCGGGTGCCCCGGGAAAGAGTAGATCGGAAGAGCAC27380.1180830013956048No Hit
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGAGATCGGAAGAGCACACG27200.11730670701097334No Hit
CGCTTGCTCTCCGGGGCCGCGGGTGAGTGTCCAGATCGGAAGAGCACACG26540.11446029426732474No Hit
CCCCTGTCCGCGGCTGGAGCGTCGCAGACAGATCGGAAGAGCACACGTCT26500.11428778440407329Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACCTCAGCTCAGACGAGAAGACCCGCTGAATTAGATCGGAAGAGCACA26490.11424465693826046No Hit
CGCTTGCTCTCCGGGGCCGCGGGTGAGTGTAGATCGGAAGAGCACACGTC25860.1115276265920504No Hit
ACGCGGCGCTCCCCCGGGGAGGGGGGAGGACAGATCGGAAGAGCACACGT25840.1114413716604247No Hit
CGACCTCAGCTCAGACGAGAAGACCCGCTGAATAGATCGGAAGAGCACAC23560.1016083094550931No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)