FastQCFastQC Report
Wed 27 Sept 2023
SRR1062227.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1062227.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Total Bases304 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGT55070413.7676No Hit
CCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGTCT1319493.298725Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CATCCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACG893992.2349750000000004No Hit
TCCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGTC651121.6278000000000001No Hit
AGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGA495241.2381Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCCGCGGACGGTGTGAGGCCGGTGTCGGCCCCCAGATCGGAAGAGCAC476201.1905000000000001No Hit
GGATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTC412501.03125No Hit
CATTGAATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGT405371.013425No Hit
AGCCCGCGGACGGTGTGAGGCCGGTGTCGGCCCCAGATCGGAAGAGCACA392530.9813250000000001No Hit
AGCCCGCGGACGGTGTGAGGCCGGTGTCGGCCCAGATCGGAAGAGCACAC383240.9581No Hit
AGCCCGCGGACGGTGTGAGGCCGGTGTCGGCCAGATCGGAAGAGCACACG329840.8246No Hit
TCTCGGCGCGGGCCGGGGGGCCAAAGTCAGATCGGAAGAGCACACGTCTG309620.77405Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGTC306890.767225No Hit
GAGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTG281530.703825Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGTCT234240.5856Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGAGATCGGAAGAGCACA228970.572425No Hit
GCCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCTG224620.56155Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACG212670.531675No Hit
CATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTC206110.515275No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGT197730.49432499999999996No Hit
GGTGGGATCCCCCCGCCCCGGCGGGGGGCGCACCACCGAGATCGGAAGAG183780.45944999999999997No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGTAGATCGGAAGAGCAC182150.455375No Hit
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGT178150.445375No Hit
ACCCGGGGACGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA175860.43965000000000004Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGAATGAGTGCATCCCAAACCCATGGGCGAAGATCGGAAGAGCACACGT156540.39135000000000003No Hit
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGTAGATCGGAAGAGCACA155350.388375No Hit
GCCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCT155330.388325Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGGAGATCGGAAGAGCAC151570.378925No Hit
CTCTCGGCGCGGGCCGGGGGGCCAAAGTCAGATCGGAAGAGCACACGTCT144070.36017499999999997Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTAAATGACTCGTAGACGACCTGATTAGATCGGAAGAGCACACGTCTGAA143200.358Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGAGATCGGAAGAGCACAC143020.35755000000000003No Hit
AACGATGCCGACCCGCGATCCGGCGGCGAGATCGGAAGAGCACACGTCTG141670.354175Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCTTGCTCTCCGGGGCCGCGGGTGAGAGATCGGAAGAGCACACGTCTGA140450.351125Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGGCGGGGGGCGCACCACCGGCCCGCCTCGCAGATCGGAAGAGCACACG136930.342325No Hit
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGT136440.3411No Hit
ATTGAATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTC129690.324225No Hit
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACG111700.27925No Hit
GGGTGGGATCCCCCCGCCCCGGCGGGGGGCGCACCACCGAGATCGGAAGA108570.271425No Hit
CTCCGGGGCCGCGGGTGAGTGTCCTCCGCGCAGATCGGAAGAGCACACGT107210.268025No Hit
AGCCCGCGGACGGTGTGAGGCCGGTGTCGGCCCCCGAGATCGGAAGAGCA104850.262125No Hit
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACAC103090.25772500000000004No Hit
CGCTTGCTCTCCGGGGCCGCGGGTGAGTGAGATCGGAAGAGCACACGTCT102790.256975Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG102240.2556TruSeq Adapter, Index 4 (100% over 49bp)
AGCCCGCGGACGGTGTGAGGCCGGTGTCGAGATCGGAAGAGCACACGTCT98240.24559999999999998Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGGAGATCGGAAGAGCACA97620.24405No Hit
CGCTTGCTCTCCGGGGCCGCGGGTGAGTAGATCGGAAGAGCACACGTCTG96620.24155000000000001Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACCTCAGCTCAGACGAGAAGACCCGCTGAAGATCGGAAGAGCACACGT96570.24142500000000003No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGAGATCGGAAGAGCACACGTC94070.23517500000000002No Hit
CCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGTCTG93470.233675Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTTTCCTCACTTACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCT91160.22790000000000002Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGTC88570.221425No Hit
GCCGAGAAGACGATCGAACTCGGTCGTAGATCGGAAGAGCACACGTCTGA87590.21897499999999998Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGTC86050.21512499999999998No Hit
CCCGGCGGGGGGCGCACCACCGGCCCGCCTCGAGATCGGAAGAGCACACG82450.206125No Hit
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGAGATCGGAAGAGCAC82250.205625No Hit
GGTGGGATCCCCCCGCCCCGGCGGGGGGCGCACCACCGGAGATCGGAAGA78340.19585000000000002No Hit
GAGCGTCGGGCCCATACCCGGCCGTCGACGGCACAAGAGATCGGAAGAGC78180.19545No Hit
GAGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACG78140.19535No Hit
CATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCTG76610.191525Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCGGCGGGGGGCGCACCACCGGCCCGCCTCGCAGATCGGAAGAGCACAC71170.177925No Hit
GGGTGGGATCCCCCCGCCCCGGCGGGGGGCGCACCACCAGATCGGAAGAG69540.17385No Hit
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGTAGATCGGAAGAGCA68810.172025No Hit
ATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTCT66120.1653Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGT66060.16515No Hit
CTCCGGGGCCGCGGGTGAGTGTCCTCCGCGCCAGATCGGAAGAGCACACG65050.16262500000000002No Hit
CCATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGT64800.16199999999999998No Hit
GCCCCGACTTCGCCGCTTGCTCTCCGGGGCCGCGGGTGAGATCGGAAGAG64000.16No Hit
GTTGAACCCCGCCCGTGATGGGGACCGGGGATTAGATCGGAAGAGCACAC63890.159725No Hit
CGTTGAACCCCGCCCGTGATGGGGACCGGGGATTGAGATCGGAAGAGCAC58220.14554999999999998No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGAGATCGGAAGAGCACAC54420.13605No Hit
ATGCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGT53420.13355No Hit
GAGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACAC52180.13045No Hit
CCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCTGA50300.12575Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTC49900.12474999999999999No Hit
CATTGAATACCCCAGCTAGGAATAATGGAAGATCGGAAGAGCACACGTCT49820.12455000000000001Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCTGCGATCCATTGAAAGTCAGCCCTCGATCCAAAGATCGGAAGAGCAC47760.11939999999999999No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCGAGATCGGAAGAGCACA47280.11820000000000001No Hit
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGGAGATCGGAAGAGCA45950.114875No Hit
CCCCTGTCCGCGGCTGGAGCGTCGCAGACAGATCGGAAGAGCACACGTCT45140.11284999999999999Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGATAACTGTGGCAATTCCAGAGCTAATACATGCAGATCGGAAGAGCA45070.112675No Hit
AGCCCGCGGACGGTGTGAGGCCGGTGTCGGAGATCGGAAGAGCACACGTC44810.112025No Hit
TCGTTTCCTCACTTACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTC44480.1112No Hit
CGACCTCAGCTCAGACGAGAAGACCCGCTGAAAGATCGGAAGAGCACACG44460.11115000000000001No Hit
TTGAATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCT43640.1091Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGAATGAGTGCATCCCAAACCCATGGGCGAAGATCGGAAGAGCACACGTC43630.10907499999999999No Hit
CGCCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCT43420.10855000000000001Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGGGGCCAAAGTCCTTCTGATGGAGGCTAGATCGGAAGAGCACACGTC43340.10835No Hit
AAACGATGCCGACCCGCGATCCGGCGGCGAGATCGGAAGAGCACACGTCT43140.10785Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTGAACCCCGCCCGTGATGGGGACCGGGGATTGAGATCGGAAGAGCACA42780.10694999999999999No Hit
GGGAGTCGGGTCCAGATCCCCGAGCCCGGAGCAGATCGGAAGAGCACACG41380.10345No Hit
TCTCCGGGGCCGCGGGTGAGTGTCCTCCGCGCAGATCGGAAGAGCACACG41350.103375No Hit
GAGCGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGT40700.10175No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)