FastQCFastQC Report
Wed 27 Sept 2023
SRR1062247.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1062247.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Total Bases304 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTGAATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGT1550393.8759750000000004No Hit
CATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTC1039642.5991No Hit
GCCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCTG666841.6670999999999998Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTC610921.5273No Hit
GCCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCT592411.481025Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGAATGAGTGCATCCCAAACCCATGGGCGAAGATCGGAAGAGCACACGT583011.457525No Hit
ATTGAATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTC559051.3976250000000001No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGTAGATCGGAAGAGCAC535861.33965No Hit
ACGATTAATAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTG526101.31525Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGT500661.25165No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGTC440471.101175No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGAGATCGGAAGAGCACA419911.049775No Hit
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGTAGATCGGAAGAGCACA411431.028575No Hit
ATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTCT393140.98285Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGT371510.9287750000000001No Hit
AGCCCGCGGACGGTGTGAGGCCGGTGTCGGCCCAGATCGGAAGAGCACAC365270.913175No Hit
CATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCTG358430.896075Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCCGCGGACGGTGTGAGGCCGGTGTCGGCCAGATCGGAAGAGCACACG328970.8224250000000001No Hit
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGAGATCGGAAGAGCACAC284740.71185No Hit
CGCCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCT257170.642925Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTC254390.635975No Hit
ACGATTAATAGTCCTACGTGATCTGAGAGATCGGAAGAGCACACGTCTGA246330.615825Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCCGCGGACGGTGTGAGGCCGGTGTCGGCCCCAGATCGGAAGAGCACA239970.599925No Hit
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGAGATCGGAAGAGCAC232100.58025No Hit
CATTGAATACCCCAGCTAGGAATAATGGAAGATCGGAAGAGCACACGTCT224920.5623Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTGAACCCCGCCCGTGATGGGGACCGGGGATTAGATCGGAAGAGCACAC224690.561725No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGT222490.5562250000000001No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACG218650.546625No Hit
CGTTGAACCCCGCCCGTGATGGGGACCGGGGATTAGATCGGAAGAGCACA210370.525925No Hit
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGTAGATCGGAAGAGCA205850.514625No Hit
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGT195320.4883No Hit
CGATTAATAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGA189300.47324999999999995Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACCTCAGCTCAGACGAGAAGACCCGCTGAAGATCGGAAGAGCACACGT185760.4644No Hit
GGAATGAGTGCATCCCAAACCCATGGGCGAAGATCGGAAGAGCACACGTC181810.45452499999999996No Hit
TTGAATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCT171970.429925Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGAGTCGGGTCCAGATCCCCGAGCCCGGAGAGATCGGAAGAGCACACG171140.42785No Hit
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGT160860.40215No Hit
CCCATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACG160070.400175No Hit
AGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGA157160.3929Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGTCAGATCGGAAGAGCA151100.37775000000000003No Hit
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACG147810.369525No Hit
CGACCTCAGCTCAGACGAGAAGACCCGCTGAAAGATCGGAAGAGCACACG136590.341475No Hit
CCATTGAATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACG135040.3376No Hit
CCCATTGAATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACAC134330.33582500000000004No Hit
ATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCTGA130290.32572500000000004Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGAACCCCGCCCGTGATGGGGACCGGGGATTGAGATCGGAAGAGCACA126160.3154No Hit
TTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTCTG124680.3117Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCTG123100.30774999999999997Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACAC121800.3045No Hit
GCCGAGAAGACGATCGAACTCGGTCGTTTAAGATCGGAAGAGCACACGTC119850.29962500000000003No Hit
CCCATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTC119790.299475No Hit
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGTCAGATCGGAAGAGCAC118590.296475No Hit
CGCCGAGAAGACGATCGAACTCGGTCGTAGATCGGAAGAGCACACGTCTG99260.24815Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACCTCAGCTCAGACGAGAAGACCCGCTGAATAGATCGGAAGAGCACAC97230.243075No Hit
GAGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACG92410.23102499999999998No Hit
GGGAGTCGGGTCCAGATCCCCGAGCCCGGAGCAGATCGGAAGAGCACACG91290.228225No Hit
CGGATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGT89960.22490000000000002No Hit
GTTGAACCCCGCCCGTGATGGGGACCGGGGATAGATCGGAAGAGCACACG88710.221775No Hit
CGCCGAGAAGACGATCGAACTCGGTCGTTTAAGATCGGAAGAGCACACGT88070.220175No Hit
CATTGAATACCCCAGCTAGGAATAATGAGATCGGAAGAGCACACGTCTGA87660.21914999999999998Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCCGCGGACGGTGTGAGGCCGGTGTCGAGATCGGAAGAGCACACGTCT87270.218175Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGTC86970.217425No Hit
CGATTAATAGTCCTACGTGATCTGAGAGATCGGAAGAGCACACGTCTGAA86150.21537499999999998Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGCCCCATTGAATACCCCAGCTAGGAATAAGATCGGAAGAGCACACGTC84620.21155000000000002No Hit
CGCTTGCTCTCCGGGGCCGCGGGTGAGTGAGATCGGAAGAGCACACGTCT81620.20405Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTGAATACCCCAGCTAGGAATAATGGAAGATCGGAAGAGCACACGTCTG81570.203925Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGAGTCGGGTCCAGATCCCCGAGCCCGGAGAGATCGGAAGAGCACACGT79400.19849999999999998No Hit
ATTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAAGTCCAGT77390.193475Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
GAGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACAC77010.192525No Hit
GGGAGTCGGGTCCAGATCCCCGAGCCCGGAGCGAGATCGGAAGAGCACAC76370.19092499999999998No Hit
AAACGATGCCGACCCGCGATCCGGCGGCGAGATCGGAAGAGCACACGTCT76340.19085Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG73000.1825TruSeq Adapter, Index 1 (100% over 49bp)
ACCCGGGGACGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA72580.18145Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAAGTCCAG72450.18112499999999998Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CATCCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACG72410.181025No Hit
GAGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTG72040.1801Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGTCT71060.17765Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCCGGGGCCGCGGGTGAGTGTCCTCCGCGCAGATCGGAAGAGCACACGT68450.171125No Hit
AAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAAGTCCA67320.1683Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
AAGGGAGTCGGGTCCAGATCCCCGAGCCCGGAGAGATCGGAAGAGCACAC65090.162725No Hit
CCCATTGAATACCCCAGCTAGGAATAATGAGATCGGAAGAGCACACGTCT63450.158625Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTTGAACCCCGCCCGTGATGGGGACCGGGGATAGATCGGAAGAGCACAC61830.154575No Hit
AGGGAGTCGGGTCCAGATCCCCGAGCCCGGAGCAGATCGGAAGAGCACAC61190.152975No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGAGATCGGAAGAGCACAC59740.14935No Hit
GAGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACA58500.14625No Hit
CGCTTGCTCTCCGGGGCCGCGGGTGAGTAGATCGGAAGAGCACACGTCTG57930.144825Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTTGAACCCCGCCCGTGATGGGGACCGGGGATTGAGATCGGAAGAGCAC57580.14395No Hit
CCCCATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGT56540.14135No Hit
CTCCGGGGCCGCGGGTGAGTGTCCTCCGCGCCAGATCGGAAGAGCACACG56010.140025No Hit
AGATCGGAAGAGCACACGTCTGAAGTCCAGTCACATCACGATCTCGTATG54650.136625TruSeq Adapter, Index 1 (97% over 49bp)
CCATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCT54320.1358Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCCGCGGACGGTGTGAGGCCGGTGTCGGCCCCCAGATCGGAAGAGCAC53060.13265No Hit
GTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAAGTCCAGT52500.13125Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
TCCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGTC51660.12915No Hit
GCCGAGAAGACGATCGAACTCGGTCGTAGATCGGAAGAGCACACGTCTGA50080.1252Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACCTCAGCTCAGACGAGAAGACCCGCTGAATTAGATCGGAAGAGCACA49450.123625No Hit
TTGGAATGAGTGCATCCCAAACCCATGGGCGAAGATCGGAAGAGCACACG48930.12232499999999999No Hit
CGCCCCATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACAC48290.120725No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGGAGATCGGAAGAGCAC47830.119575No Hit
AGGGAGTCGGGTCCAGATCCCCGAGCCCGGAGCGAGATCGGAAGAGCACA46980.11745No Hit
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGAGATCGGAAGAGCACACG46430.11607500000000001No Hit
CCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGTCT45060.11264999999999999Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGAAGGGCAGGGCGCCCTGGAACGGGTTAGATCGGAAGAGCACACGTCT44190.110475Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACGATTAATAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCT43870.10967500000000001Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTAATAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGAAC43380.10844999999999999Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAAGTCC43370.108425Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGGAGATCGGAAGAGCACA41490.103725No Hit
TAAGCTGTAGCTAAATCTAGACAGATCGGAAGAGCACACGTCTGAAGTCC41380.10345Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
TTGGAGCGCGCGCGATGGCACCCGAAAAGATCGGAAGAGCACACGTCTGA40710.101775Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)