FastQCFastQC Report
Wed 27 Sept 2023
SRR1062287.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1062287.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Total Bases304 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTC1745864.36465No Hit
CATTGAATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGT1433943.58485No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGT861072.152675No Hit
CATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCTG845382.1134500000000003Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGT610821.52705No Hit
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGT565181.41295No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGTC562431.406075No Hit
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGT554091.385225No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACG480771.2019250000000001No Hit
ACGATTAATAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTG469231.173075Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACG468851.172125No Hit
GGATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTC430511.0762749999999999No Hit
ATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTCT429401.0735Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCT356770.891925Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CATTGAATACCCCAGCTAGGAATAATGGAAGATCGGAAGAGCACACGTCT352340.88085Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTGAATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTC333950.834875No Hit
CCCATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTC333570.8339249999999999No Hit
GCCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCTG306450.766125Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGA237040.5926Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG225310.563275TruSeq Adapter, Index 8 (100% over 49bp)
CGCCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCT225280.5632Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCT216790.541975Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGTC210750.526875No Hit
AGCCCGCGGACGGTGTGAGGCCGGTGTCGGCCAGATCGGAAGAGCACACG199710.49927499999999997No Hit
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGTC199080.4977No Hit
ATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCTGA174040.4351Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGAACCCCGCCCGTGATGGGGACCGGGGATTAGATCGGAAGAGCACAC167600.41900000000000004No Hit
CGCCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTC162820.40705No Hit
GAGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTG157810.394525Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCTG155370.388425Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTCTG146740.36685Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGAATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCT132590.331475Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGTCT131840.3296Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGAGAAGACGATCGAACTCGGTCGTAGATCGGAAGAGCACACGTCTG127190.317975Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGAATGAGTGCATCCCAAACCCATGGGCGAAGATCGGAAGAGCACACGT123950.309875No Hit
AATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCTGAA110550.27637500000000004Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTCTG110120.2753Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCGAGAAGACGATCGAACTCGGTCGTTTAAGATCGGAAGAGCACACGTC106680.2667No Hit
CCCATTGAATACCCCAGCTAGGAATAATGAGATCGGAAGAGCACACGTCT103180.25795Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CATTGAATACCCCAGCTAGGAATAATGAGATCGGAAGAGCACACGTCTGA101060.25265Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGT96170.240425No Hit
AAACGATGCCGACCCGCGATCCGGCGGCGAGATCGGAAGAGCACACGTCT94880.2372Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGATTAATAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGA92600.23149999999999998Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGCCTACGTCCTCCTAAGCGCAGACCGAGATCGGAAGAGCACACGTCT91840.2296Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGAGATCGGAAGAGCACAC88130.220325No Hit
AATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTCTGAAC87530.21882500000000002Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGAATGAGTGCATCCCAAACCCATGGGCGAAGATCGGAAGAGCACACGTC86510.21627500000000002No Hit
ACGATTAATAGTCCTACGTGATCTGAGAGATCGGAAGAGCACACGTCTGA86300.21575000000000003Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTGCGGTGACGCAAACGAACCCGGAGAAGATCGGAAGAGCACACGTCTG86230.21557500000000002Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGCCCCATTGAATACCCCAGCTAGGAATAAGATCGGAAGAGCACACGTC85780.21445No Hit
AGCCCGCGGACGGTGTGAGGCCGGTGTCGAGATCGGAAGAGCACACGTCT82690.20672500000000002Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACG81380.20345No Hit
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGAGATCGGAAGAGCACACG79580.19895No Hit
ATTGAATACCCCAGCTAGGAATAATGGAAGATCGGAAGAGCACACGTCTG73370.183425Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGTCT69130.172825Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACGATTAATAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCT67120.1678Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGCCTGGGAATACCAGGTGCTGTAAGAGATCGGAAGAGCACACGTCTG66460.16615Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGGCGCAATGAAGGTGAGAACGGGGGAGATCGGAAGAGCACACGTCTGA64620.16155Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGCGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGT64550.161375No Hit
CAGTGCGGTGACGCAAACGAACCCGGAGAAGATCGGAAGAGCACACGTCT61920.1548Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTGAACCCCGCCCGTGATGGGGACCGGGGATAGATCGGAAGAGCACACG61230.153075No Hit
ACTGTGGCAATTCCAGAGCTAATACATGCAGATCGGAAGAGCACACGTCT60830.152075Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCTGAA59210.148025Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGTCT58280.1457Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTGCGGTGACGCAAACGAACCCGGAGATAGATCGGAAGAGCACACGTCT56410.141025Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCCATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGT56290.14072500000000002No Hit
CGCCGAGAAGACGATCGAACTCGGTCGTTTAAGATCGGAAGAGCACACGT55990.139975No Hit
CCATTGAATACCCCAGCTAGGAATAATGGAAGATCGGAAGAGCACACGTC54940.13735No Hit
CCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCTGA51230.128075Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGAGAAGACGATCGAACTCGGTCGTTTAAGATCGGAAGAGCACACGTCT50430.126075Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCTG48660.12165Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGAGTCGGGTCCAGATCCCCGAGCCCGGAGAGATCGGAAGAGCACACG48580.12145No Hit
CAGTGCGGTGACGCAAACGAACCCGGAGAGATCGGAAGAGCACACGTCTG47750.119375Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCCGCGGACGGTGTGAGGCCGGTGTCGGAGATCGGAAGAGCACACGTC47400.11850000000000001No Hit
AGCCCGCGGACGGTGTGAGGCCGGTGTCAGATCGGAAGAGCACACGTCTG46700.11674999999999999Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTTTCCTCACTTACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCT46300.11574999999999999Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACTGTGGCAATTCCAGAGCTAATACATGCAGATCGGAAGAGCACACGTC45980.11495No Hit
CGCTTGCTCTCCGGGGCCGCGGGTGAGTGAGATCGGAAGAGCACACGTCT45430.11357500000000001Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCCGGGGCCGCGGGTGAGTGTCCTCCGCGCAGATCGGAAGAGCACACGT44990.112475No Hit
TCCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGTC44240.1106No Hit
AGTGCGGTGACGCAAACGAACCCGGAGATGAGATCGGAAGAGCACACGTC44240.1106No Hit
GCCGAGAAGACGATCGAACTCGGTCGTAGATCGGAAGAGCACACGTCTGA44120.11030000000000001Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGCGGCGCAATGAAGGTGAGAACGGGGGAGATCGGAAGAGCACACGTCTG40560.10139999999999999Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)