FastQCFastQC Report
Wed 27 Sept 2023
SRR1062298.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1062298.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Total Bases304 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG1825574.563925TruSeq Adapter, Index 10 (100% over 49bp)
ACCGCCTGGGAATACCAGGTGCTGTAAGAGATCGGAAGAGCACACGTCTG1712614.281525Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGT403791.009475No Hit
ATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTCTG375010.9375249999999999Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGCCTACGTCCTCCTAAGCGCAGACCGAGATCGGAAGAGCACACGTCT346580.86645Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGCCTGGGAATACCAGGTGCTGTAAAGATCGGAAGAGCACACGTCTGA293140.73285Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTCGGTCGGTGCGGAGAGCCAGCCGTGAGATCGGAAGAGCACACGTCTG248580.6214500000000001Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCTTTCGAACGCACATTGCGGCCCCGGGTAGATCGGAAGAGCACACGTC223050.5576249999999999No Hit
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGTC180170.45042499999999996No Hit
AACTCTTAGCGGTGGATCACTCGGCTCGAGATCGGAAGAGCACACGTCTG156400.391Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTC155500.38875No Hit
ACCTTTCGAACGCACATTGCGGCCCCGGGAGATCGGAAGAGCACACGTCT154660.38665Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTTCGCCGCTTGCTCTCCGGGGCCGCGGGTAGATCGGAAGAGCACACGT152870.382175No Hit
CGCCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCT151950.379875Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACG148950.372375No Hit
CCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGTCT143600.359Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGTCTG139190.34797500000000003Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACG135660.33915No Hit
AACTCTTAGCGGTGGATCACTCGGAGATCGGAAGAGCACACGTCTGAACT120040.30010000000000003Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACACTTCGAACGCACTTTGCGGCCCCGGAGATCGGAAGAGCACACGTCTG116780.29195Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCTG114030.285075Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACCGCCTGGGAATACCAGGTGCTGTAAGAGATCGGAAGAGCACACGTCT113910.284775Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGT112450.281125No Hit
AACTCTTAGCGGTGGATCACTCGGCTCGTGAGATCGGAAGAGCACACGTC112080.2802No Hit
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGTCT107570.26892499999999997Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGTCTG105140.26285000000000003Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGT104540.26134999999999997No Hit
CCTTTCGAACGCACATTGCGGCCCCGGGAGATCGGAAGAGCACACGTCTG100900.25225Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGAAC97250.24312499999999998Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTC95440.2386No Hit
CGGATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGT94940.23735No Hit
AGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGA94130.23532499999999998Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACGTC92020.23005No Hit
TACCTGGTTGATCCTGCCAGTAACAGATCGGAAGAGCACACGTCTGAACT88440.2211Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGTC86640.2166No Hit
ACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGAACTC83180.20795000000000002Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACTTCGAACGCACTTTGCGGCCCCGGAGATCGGAAGAGCACACGTCTGAA82640.2066Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGCCTGGGAATACCAGGTGCTGTAAGAGATCGGAAGAGCACACGTCTGA81600.20400000000000001Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTCGGTCGGTGCGGAGAGCCAGCCGAGATCGGAAGAGCACACGTCTGAA81000.20249999999999999Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTCT80480.2012Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGCGGGGGGCGCACCACCGGCCCGTCTCGTAGATCGGAAGAGCACACGT78680.19669999999999999No Hit
CGGGGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGA77240.1931Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCT69940.17485Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGAACT69770.174425Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCCATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTC69050.172625No Hit
GAGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTG67310.168275Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGACCGCCTGGGAATACCAGGTGCTGTAAGAGATCGGAAGAGCACACGTC64990.16247499999999998No Hit
TCTCTCGGCGCGGGCCGGGGGGCCAAAAGATCGGAAGAGCACACGTCTGA61320.15330000000000002Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTCGGTCGGTGCGGAGAGCCAGCCGTAGATCGGAAGAGCACACGTCTGA60770.151925Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGCCTGGGAATACCAGGTGCTGAGATCGGAAGAGCACACGTCTGAACT59830.14957499999999999Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGTCTGAA58780.14695Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGGGTCAGGGGGCGAAGGTGGCCCGTCGGAGATCGGAAGAGCACACGTC58730.14682499999999998No Hit
ACCGCCTGGGAATACCAGGTGCTGTAAGATCGGAAGAGCACACGTCTGAA58710.14677500000000002Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCTGA57660.14415Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGCGAGAACTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTGAA57460.14365Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGAGAAGACGATCGAACTCGGTCGTTTAAGATCGGAAGAGCACACGTCT56640.1416Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGTCT51850.12962500000000002Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTC51410.128525No Hit
CCGCTTGCTACCCGGGGCCGCGGGCAAGATCGGAAGAGCACACGTCTGAA50610.126525Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGTC49980.12494999999999999No Hit
CAACTCTTAGCGGTGGATCACTCGGCTCGAGATCGGAAGAGCACACGTCT49240.12310000000000001Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGAACCCCGCCCGTGATGGGGACCGGGGATTAGATCGGAAGAGCACACG47840.1196No Hit
ACGATTAATAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTG47780.11945Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTCTGA46710.11677499999999999Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACCCCGCCCGTGATGGGGACCGGGGATTAGATCGGAAGAGCACACGTCT46620.11655000000000001Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTTCTACAGTCCGACGATCAAACAGATCGGAAGAGCACACGTCTGAACT46110.11527499999999999Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTTGAACCCCGCCCGTGATGGGGACCGGGGATTAGATCGGAAGAGCACAC45620.11405No Hit
TGAAACTGCGAATGGCTCATTAAATCAGAGATCGGAAGAGCACACGTCTG44720.11180000000000001Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTTCGCCGCTTGCTCTCCGGGGCCGCGGGTGAGATCGGAAGAGCACACG43770.109425No Hit
CTTCGCCGCTTGCTCTCCGGGGCCGCGGGTAGATCGGAAGAGCACACGTC43550.10887500000000001No Hit
CGCCCCCTCGCCCCGACTTCGCCGCTTGCAGATCGGAAGAGCACACGTCT43430.10857499999999999Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACGATGCCGACCCGCGATCCGGCGGCGAGATCGGAAGAGCACACGTCT43290.10822500000000002Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTTCGGGGGCGAAGGTGGCTCGCGGCTAGATCGGAAGAGCACACGTCTG42980.10744999999999999Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGTC42120.10529999999999999No Hit
GTTGAACCCCGCCCGTGATGGGGACCGGGGATAGATCGGAAGAGCACACG42030.105075No Hit
TTTCCTCACTTACCCGGTTGGGCAGGGAGATCGGAAGAGCACACGTCTGA41480.1037Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGTCTGAAC41430.103575Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACACTTCGAACGCACTTTGCGGCCCCGGGAGATCGGAAGAGCACACGTCT41250.10312500000000001Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCTTTCGAACGCACATTGCGGCCCCGGAGATCGGAAGAGCACACGTCTG41100.10275Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCTG40770.101925Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGGGGGGCGCACCACCGGCCCGTCTCGTAGATCGGAAGAGCACACGTCT40520.1013Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)