FastQCFastQC Report
Wed 27 Sept 2023
SRR1062301.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1062301.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Total Bases304 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG1802784.50695TruSeq Adapter, Index 10 (100% over 49bp)
ACCGCCTGGGAATACCAGGTGCTGTAAGAGATCGGAAGAGCACACGTCTG1668134.170325Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGT386500.9662499999999999No Hit
ATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTCTG374040.9351Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGCCTACGTCCTCCTAAGCGCAGACCGAGATCGGAAGAGCACACGTCT340400.851Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGCCTGGGAATACCAGGTGCTGTAAAGATCGGAAGAGCACACGTCTGA287300.7182499999999999Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTCGGTCGGTGCGGAGAGCCAGCCGTGAGATCGGAAGAGCACACGTCTG246150.615375Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCTTTCGAACGCACATTGCGGCCCCGGGTAGATCGGAAGAGCACACGTC219790.5494749999999999No Hit
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGTC172790.431975No Hit
AACTCTTAGCGGTGGATCACTCGGCTCGAGATCGGAAGAGCACACGTCTG153790.384475Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTC153180.38295No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACG151000.3775No Hit
ACTTCGCCGCTTGCTCTCCGGGGCCGCGGGTAGATCGGAAGAGCACACGT150140.37534999999999996No Hit
ACCTTTCGAACGCACATTGCGGCCCCGGGAGATCGGAAGAGCACACGTCT148640.3716Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCT146910.36727499999999996Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGTCT143630.35907500000000003Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGTCTG134550.336375Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACG130960.3274No Hit
ACACTTCGAACGCACTTTGCGGCCCCGGAGATCGGAAGAGCACACGTCTG120740.30185Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACTCTTAGCGGTGGATCACTCGGAGATCGGAAGAGCACACGTCTGAACT119310.298275Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCTG117800.29450000000000004Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACCGCCTGGGAATACCAGGTGCTGTAAGAGATCGGAAGAGCACACGTCT115480.28869999999999996Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGT114690.286725No Hit
AACTCTTAGCGGTGGATCACTCGGCTCGTGAGATCGGAAGAGCACACGTC108960.2724No Hit
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGTCT104300.26075Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGT101750.254375No Hit
CGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGTCTG101070.252675Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTTTCGAACGCACATTGCGGCCCCGGGAGATCGGAAGAGCACACGTCTG98930.24732500000000002Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGAAC97180.24294999999999997Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGT96740.24185No Hit
CGCCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTC94360.2359No Hit
AGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGA93260.23314999999999997Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACGTC92980.23245000000000002No Hit
TACCTGGTTGATCCTGCCAGTAACAGATCGGAAGAGCACACGTCTGAACT89780.22444999999999998Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGTC84490.211225No Hit
ACTTCGAACGCACTTTGCGGCCCCGGAGATCGGAAGAGCACACGTCTGAA82340.20585Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGCCTGGGAATACCAGGTGCTGTAAGAGATCGGAAGAGCACACGTCTGA82250.205625Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGAACTC82180.20545Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTCT80360.2009Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGA80060.20015Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTCGGTCGGTGCGGAGAGCCAGCCGAGATCGGAAGAGCACACGTCTGAA79470.198675Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGCGGGGGGCGCACCACCGGCCCGTCTCGTAGATCGGAAGAGCACACGT75960.1899No Hit
GACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGAACT70240.1756Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCT69210.173025Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTC68410.17102499999999998No Hit
GAGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTG66630.166575Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGACCGCCTGGGAATACCAGGTGCTGTAAGAGATCGGAAGAGCACACGTC64520.1613No Hit
TCTCTCGGCGCGGGCCGGGGGGCCAAAAGATCGGAAGAGCACACGTCTGA62360.1559Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGCGAGAACTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTGAA60760.1519Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCTGA60230.150575Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGGGTCAGGGGGCGAAGGTGGCCCGTCGGAGATCGGAAGAGCACACGTC59570.148925No Hit
CCTCGGTCGGTGCGGAGAGCCAGCCGTAGATCGGAAGAGCACACGTCTGA59250.148125Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGTCTGAA57930.144825Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCGCCTGGGAATACCAGGTGCTGAGATCGGAAGAGCACACGTCTGAACT57130.142825Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGAGAAGACGATCGAACTCGGTCGTTTAAGATCGGAAGAGCACACGTCT57130.142825Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGCCTGGGAATACCAGGTGCTGTAAGATCGGAAGAGCACACGTCTGAA56110.14027499999999998Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGAACCCCGCCCGTGATGGGGACCGGGGATTAGATCGGAAGAGCACACG52790.131975No Hit
CATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTC52020.13005No Hit
CCGCTTGCTACCCGGGGCCGCGGGCAAGATCGGAAGAGCACACGTCTGAA51680.1292Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAACTCTTAGCGGTGGATCACTCGGCTCGAGATCGGAAGAGCACACGTCT49930.124825Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGTC49650.124125No Hit
CCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGTCT49400.1235Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTCTGA48650.12162500000000001Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGATTAATAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTG47260.11815Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACCCCGCCCGTGATGGGGACCGGGGATTAGATCGGAAGAGCACACGTCT45660.11414999999999999Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAAACTGCGAATGGCTCATTAAATCAGAGATCGGAAGAGCACACGTCTG45460.11364999999999999Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAACGATGCCGACCCGCGATCCGGCGGCGAGATCGGAAGAGCACACGTCT45410.113525Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTGAACCCCGCCCGTGATGGGGACCGGGGATTAGATCGGAAGAGCACAC45170.112925No Hit
AGTTCTACAGTCCGACGATCAAACAGATCGGAAGAGCACACGTCTGAACT45120.11280000000000001Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGTC43780.10944999999999999No Hit
TTTCCTCACTTACCCGGTTGGGCAGGGAGATCGGAAGAGCACACGTCTGA43380.10844999999999999Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTCGCCGCTTGCTCTCCGGGGCCGCGGGTAGATCGGAAGAGCACACGTC42670.10667499999999999No Hit
GTTGAACCCCGCCCGTGATGGGGACCGGGGATAGATCGGAAGAGCACACG42330.10582499999999999No Hit
ACACTTCGAACGCACTTTGCGGCCCCGGGAGATCGGAAGAGCACACGTCT42010.105025Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTTCGCCGCTTGCTCTCCGGGGCCGCGGGTGAGATCGGAAGAGCACACG41370.103425No Hit
CATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCTG40860.10215Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGGGGGGCGCACCACCGGCCCGTCTCGTAGATCGGAAGAGCACACGTCT40430.101075Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCCCCTCGCCCCGACTTCGCCGCTTGCAGATCGGAAGAGCACACGTCT40120.1003Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)