FastQCFastQC Report
Wed 27 Sept 2023
SRR1062315.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1062315.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2727752
Total Bases207.3 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTGAATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGT1212024.443292498731556No Hit
CATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTC810632.971787757831357No Hit
GCCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCTG485731.780697072167851Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGAATGAGTGCATCCCAAACCCATGGGCGAAGATCGGAAGAGCACACGT467811.7150019503239295No Hit
GGATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTC452141.6575553789347417No Hit
GCCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCT435511.596589426018201Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTGAATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTC428751.5718071144297574No Hit
ACGATTAATAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTG387911.4220867586202852Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGT350091.2834377905322771No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGTAGATCGGAAGAGCAC341671.2525698817194526No Hit
ATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTCT294881.0810366924852406Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGTC287381.053541524302796No Hit
CCATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGT285941.0482624520117665No Hit
CATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCTG273131.0013007047561509Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGAGATCGGAAGAGCACA272140.9976713425560682No Hit
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGTAGATCGGAAGAGCACA250220.9173121310148431No Hit
AGCCCGCGGACGGTGTGAGGCCGGTGTCGGCCCAGATCGGAAGAGCACAC243960.8943628306385624No Hit
AGCCCGCGGACGGTGTGAGGCCGGTGTCGGCCAGATCGGAAGAGCACACG216610.7940971173332473No Hit
ACGATTAATAGTCCTACGTGATCTGAGAGATCGGAAGAGCACACGTCTGA181840.6666295176394335Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGAGATCGGAAGAGCACAC171120.6273297572506591No Hit
CATTGAATACCCCAGCTAGGAATAATGGAAGATCGGAAGAGCACACGTCT170050.6234071132566303Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTC167650.614608659438248No Hit
CGCCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCT165190.6055902442744062Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTGAACCCCGCCCGTGATGGGGACCGGGGATTAGATCGGAAGAGCACAC165140.6054069431531899No Hit
AGCCCGCGGACGGTGTGAGGCCGGTGTCGGCCCCAGATCGGAAGAGCACA160970.5901196296437505No Hit
CGTTGAACCCCGCCCGTGATGGGGACCGGGGATTAGATCGGAAGAGCACA152590.559398361727899No Hit
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGAGATCGGAAGAGCAC148340.5438177664245137No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGT148200.543304523285108No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACG146990.5388686361516736No Hit
CGATTAATAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGA141630.5192187559572865Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGAATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCT136650.5009619642841431Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGAATGAGTGCATCCCAAACCCATGGGCGAAGATCGGAAGAGCACACGTC133880.49080708216876023No Hit
CGACCTCAGCTCAGACGAGAAGACCCGCTGAAGATCGGAAGAGCACACGT128630.47156046444104893No Hit
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGTAGATCGGAAGAGCA125730.4609289994105036No Hit
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGT125070.45850942461044847No Hit
CCCATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACG114800.4208593743126208No Hit
AGGGAGTCGGGTCCAGATCCCCGAGCCCGGAGAGATCGGAAGAGCACACG114510.4197962278095662No Hit
AGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGA108810.39889989999090825Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCATTGAATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACG101120.37070818754784157No Hit
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGT99040.36308286090524355No Hit
CCCATTGAATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACAC98160.35985676117183674No Hit
ATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCTGA98080.3595634793778907Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGTCAGATCGGAAGAGCA97850.3587202942202957No Hit
CGACCTCAGCTCAGACGAGAAGACCCGCTGAAAGATCGGAAGAGCACACG97570.3576938079414844No Hit
TTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTCTG96720.3545776888808074Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACG93790.3438362431775323No Hit
GTTGAACCCCGCCCGTGATGGGGACCGGGGATTGAGATCGGAAGAGCACA93440.34255313532901815No Hit
GCCGAGAAGACGATCGAACTCGGTCGTTTAAGATCGGAAGAGCACACGTC89090.32660593778320024No Hit
CCCATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTC85710.31421478198897845No Hit
CCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCTG84200.3086790881282463Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACAC82050.3007971399159455No Hit
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGTCAGATCGGAAGAGCAC73700.27018585267282363No Hit
CGACCTCAGCTCAGACGAGAAGACCCGCTGAATAGATCGGAAGAGCACAC69210.2537254119876No Hit
CATTGAATACCCCAGCTAGGAATAATGAGATCGGAAGAGCACACGTCTGA68260.2502426906844904Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGT68100.24965612709659823No Hit
GTTGAACCCCGCCCGTGATGGGGACCGGGGATAGATCGGAAGAGCACACG67240.2465033478116779No Hit
CGCCGAGAAGACGATCGAACTCGGTCGTAGATCGGAAGAGCACACGTCTG65290.23935460408424225Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGAGTCGGGTCCAGATCCCCGAGCCCGGAGCAGATCGGAAGAGCACACG64390.2360551839023489No Hit
CGATTAATAGTCCTACGTGATCTGAGAGATCGGAAGAGCACACGTCTGAA63520.2328657443931853Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACG61740.22634022447788507No Hit
ATTGAATACCCCAGCTAGGAATAATGGAAGATCGGAAGAGCACACGTCTG61320.22480049505966818Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGCCCCATTGAATACCCCAGCTAGGAATAAGATCGGAAGAGCACACGTC60270.2209511715141259No Hit
CGCCGAGAAGACGATCGAACTCGGTCGTTTAAGATCGGAAGAGCACACGT57310.21009974513812105No Hit
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGTC57210.20973314289568848No Hit
CGCTTGCTCTCCGGGGCCGCGGGTGAGTGAGATCGGAAGAGCACACGTCT56270.20628708181682207Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCCGCGGACGGTGTGAGGCCGGTGTCGAGATCGGAAGAGCACACGTCT55980.20522393531376754Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGAGTCGGGTCCAGATCCCCGAGCCCGGAGAGATCGGAAGAGCACACGT55280.20265771961673934No Hit
GGGAGTCGGGTCCAGATCCCCGAGCCCGGAGCGAGATCGGAAGAGCACAC53860.19745196777419646No Hit
AAACGATGCCGACCCGCGATCCGGCGGCGAGATCGGAAGAGCACACGTCT52740.1933460226589514Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CATCCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACG52590.1927961192953025No Hit
GAGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACAC51170.18759036745275962No Hit
GAGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTG50770.18612395848302926Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGGAGATCGGAAGAGCAC48300.17706888309494412No Hit
ACCCGGGGACGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA47150.17285295730696926Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGGGAGTCGGGTCCAGATCCCCGAGCCCGGAGAGATCGGAAGAGCACAC46170.16926025533112982No Hit
CCCATTGAATACCCCAGCTAGGAATAATGAGATCGGAAGAGCACACGTCT46120.1690769542099135Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGTCT45650.1673539236704803Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCCGGGGCCGCGGGTGAGTGTCCTCCGCGCAGATCGGAAGAGCACACGT45280.1659974953734797No Hit
CGTTGAACCCCGCCCGTGATGGGGACCGGGGATAGATCGGAAGAGCACAC44880.1645310864037493No Hit
CGTTGAACCCCGCCCGTGATGGGGACCGGGGATTGAGATCGGAAGAGCAC42980.15756564379753No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG42480.155732632585367TruSeq Adapter, Index 1 (100% over 49bp)
AGGGAGTCGGGTCCAGATCCCCGAGCCCGGAGCAGATCGGAAGAGCACAC42110.15437620428836638No Hit
CCATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCT40890.14990365693068872Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACA40320.14781402414882291No Hit
TCCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGTC39920.14634761517909253No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGAGATCGGAAGAGCACAC39730.1456510709184706No Hit
CCCCATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGT38900.14260827230628004No Hit
CGCTTGCTCTCCGGGGCCGCGGGTGAGTAGATCGGAAGAGCACACGTCTG38880.14253495185779352Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGAATGAGTGCATCCCAAACCCATGGGCGAAGATCGGAAGAGCACACG37410.13714589889403436No Hit
CTCCGGGGCCGCGGGTGAGTGTCCTCCGCGCCAGATCGGAAGAGCACACG37240.13652267508189894No Hit
CGACCTCAGCTCAGACGAGAAGACCCGCTGAATTAGATCGGAAGAGCACA36970.13553284902733095No Hit
GCCGAGAAGACGATCGAACTCGGTCGTAGATCGGAAGAGCACACGTCTGA35920.1316835254817887Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCCGCGGACGGTGTGAGGCCGGTGTCGGCCCCCAGATCGGAAGAGCAC35020.1283841052998953No Hit
ATTAATAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGAAC32470.11903574811786409Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AACGATTAATAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCT32040.11745935847540392Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGGAGATCGGAAGAGCACA31800.11657951309356569No Hit
GTGAAGGGCAGGGCGCCCTGGAACGGGTTAGATCGGAAGAGCACACGTCT31120.11408661784502404Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCCCATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACAC31020.11372001560259143No Hit
TTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCTGAA30670.11243690775407736Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGGAGCGCGCGCGATGGCACCCGAAAAGATCGGAAGAGCACACGTCTGA30560.11203364528740149Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGTCT30250.11089717833586044Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGATAACTGTGGCAATTCCAGAGCTAATACAGATCGGAAGAGCACACG30010.11001733295402222No Hit
AGGGAGTCGGGTCCAGATCCCCGAGCCCGGAGCGAGATCGGAAGAGCACA29980.10990735228129243No Hit
GATTAATAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGAA28830.10569142649331759Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGAGATCGGAAGAGCACACG28830.10569142649331759No Hit
TCTCTCGGCGCGGGCCGGGGGGCCAAAGTCAGATCGGAAGAGCACACGTC27720.10162214160231574No Hit
TTAAATGACTCGTAGACGACCTGATTAGATCGGAAGAGCACACGTCTGAA27340.10022905308107188Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)