FastQCFastQC Report
Wed 27 Sept 2023
SRR1062396.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1062396.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Total Bases304 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTC1800534.501325No Hit
CATTGAATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGT1479873.699675No Hit
CATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCTG857952.144875Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGT767331.9183249999999998No Hit
CCATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGT628861.57215No Hit
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGT532841.3321No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGTC512341.28085No Hit
ACGATTAATAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTG506391.265975Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGT492471.231175No Hit
ATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTCT463201.158Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACG448841.1221No Hit
GGATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTC430701.0767499999999999No Hit
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACG419621.04905No Hit
CATTGAATACCCCAGCTAGGAATAATGGAAGATCGGAAGAGCACACGTCT360780.90195Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTGAATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTC360640.9016No Hit
GCCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCT332030.8300750000000001Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTC317700.79425No Hit
GCCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCTG273620.68405Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGA256400.641Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCT225140.56285Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCT224130.560325Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGTC193310.483275No Hit
AGCCCGCGGACGGTGTGAGGCCGGTGTCGGCCAGATCGGAAGAGCACACG189960.4749No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG187070.46767500000000006TruSeq Adapter, Index 8 (100% over 49bp)
ATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCTGA180870.452175Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTCTG176080.4402Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTGAACCCCGCCCGTGATGGGGACCGGGGATTAGATCGGAAGAGCACAC168630.421575No Hit
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGTC164880.41219999999999996No Hit
GAGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTG162890.40722500000000006Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTC161720.4043No Hit
TTGAATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCT156820.39205Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCTG152690.381725Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGAATGAGTGCATCCCAAACCCATGGGCGAAGATCGGAAGAGCACACGT137640.3441No Hit
CGCCGAGAAGACGATCGAACTCGGTCGTAGATCGGAAGAGCACACGTCTG127660.31915Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGTCT115050.28762499999999996Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCTGAA111410.278525Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTCTG110840.2771Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CATTGAATACCCCAGCTAGGAATAATGAGATCGGAAGAGCACACGTCTGA104380.26094999999999996Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGAGAAGACGATCGAACTCGGTCGTTTAAGATCGGAAGAGCACACGTC101320.2533No Hit
CCCATTGAATACCCCAGCTAGGAATAATGAGATCGGAAGAGCACACGTCT97970.244925Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGT97550.243875No Hit
CGATTAATAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGA96440.24109999999999998Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGCCTACGTCCTCCTAAGCGCAGACCGAGATCGGAAGAGCACACGTCT95340.23835Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGATTAATAGTCCTACGTGATCTGAGAGATCGGAAGAGCACACGTCTGA92790.23197500000000001Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTCTGAAC90140.22535Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTGCGGTGACGCAAACGAACCCGGAGAAGATCGGAAGAGCACACGTCTG87660.21914999999999998Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGCCCCATTGAATACCCCAGCTAGGAATAAGATCGGAAGAGCACACGTC87470.21867499999999998No Hit
AAACGATGCCGACCCGCGATCCGGCGGCGAGATCGGAAGAGCACACGTCT86860.21714999999999998Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGAATGAGTGCATCCCAAACCCATGGGCGAAGATCGGAAGAGCACACGTC83830.20957499999999998No Hit
ATTGAATACCCCAGCTAGGAATAATGGAAGATCGGAAGAGCACACGTCTG80140.20035Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCCGCGGACGGTGTGAGGCCGGTGTCGAGATCGGAAGAGCACACGTCT77400.1935Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACG76300.19075No Hit
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGAGATCGGAAGAGCACAC75290.188225No Hit
AACGATTAATAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCT68710.171775Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGAGATCGGAAGAGCACACG67350.168375No Hit
TTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCTGAA66790.166975Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCGCCTGGGAATACCAGGTGCTGTAAGAGATCGGAAGAGCACACGTCTG65450.163625Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTGTGGCAATTCCAGAGCTAATACATGCAGATCGGAAGAGCACACGTCT65010.162525Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGGCGCAATGAAGGTGAGAACGGGGGAGATCGGAAGAGCACACGTCTGA64940.16235Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTTTCCTCACTTACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCT63950.159875Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAGTGCGGTGACGCAAACGAACCCGGAGAAGATCGGAAGAGCACACGTCT63050.157625Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGTCT60510.151275Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTGAACCCCGCCCGTGATGGGGACCGGGGATAGATCGGAAGAGCACACG60170.150425No Hit
GAGCGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGT59420.14855000000000002No Hit
AGTGCGGTGACGCAAACGAACCCGGAGATAGATCGGAAGAGCACACGTCT58220.14554999999999998Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCATTGAATACCCCAGCTAGGAATAATGGAAGATCGGAAGAGCACACGTC56500.14125No Hit
CGCCGAGAAGACGATCGAACTCGGTCGTTTAAGATCGGAAGAGCACACGT55630.139075No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGTCT53940.13485Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGAGTCGGGTCCAGATCCCCGAGCCCGGAGAGATCGGAAGAGCACACG53710.134275No Hit
TGAATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCTG52040.1301Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGAGAAGACGATCGAACTCGGTCGTTTAAGATCGGAAGAGCACACGTCT51460.12865000000000001Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCCATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGT51310.128275No Hit
CCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCTGA51250.12812500000000002Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACTGTGGCAATTCCAGAGCTAATACATGCAGATCGGAAGAGCACACGTC49110.122775No Hit
CAGTGCGGTGACGCAAACGAACCCGGAGAGATCGGAAGAGCACACGTCTG48840.12210000000000001Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTGCGGTGACGCAAACGAACCCGGAGATGAGATCGGAAGAGCACACGTC46620.11655000000000001No Hit
AGCCCGCGGACGGTGTGAGGCCGGTGTCGGAGATCGGAAGAGCACACGTC44010.11002500000000001No Hit
CTGTGGCAATTCCAGAGCTAATACATGCAGATCGGAAGAGCACACGTCTG43250.108125Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCCGCGGACGGTGTGAGGCCGGTGTCAGATCGGAAGAGCACACGTCTG42870.107175Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCTTGCTCTCCGGGGCCGCGGGTGAGTGAGATCGGAAGAGCACACGTCT42190.10547499999999999Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCGGCGCAATGAAGGTGAGAACGGGGGAGATCGGAAGAGCACACGTCTG41970.104925Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGTCAGGGGGCGAAGGTGGCCCGTCGGAGATCGGAAGAGCACACGTCT41740.10435Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGTC41730.10432499999999999No Hit
GCCGAGAAGACGATCGAACTCGGTCGTAGATCGGAAGAGCACACGTCTGA41630.104075Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCCGGGGCCGCGGGTGAGTGTCCTCCGCGCAGATCGGAAGAGCACACGT41320.1033No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)