FastQCFastQC Report
Wed 27 Sept 2023
SRR1062397.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1062397.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Total Bases304 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTC1809534.523825No Hit
CATTGAATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGT1485133.712825No Hit
CATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCTG864302.16075Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGT716391.7909749999999998No Hit
CCATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGT620911.552275No Hit
ACGATTAATAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTG508621.27155Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGT485931.2148249999999998No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGTC471241.1781No Hit
ATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTCT447031.117575Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGT440241.1006No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACG415491.0387250000000001No Hit
GGATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTC412501.03125No Hit
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACG384920.9622999999999999No Hit
CATTGAATACCCCAGCTAGGAATAATGGAAGATCGGAAGAGCACACGTCT359770.899425Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTGAATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTC346070.865175No Hit
GCCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCT304200.7605Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTC299570.7489250000000001No Hit
GCCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCTG251180.62795Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGA235590.588975Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCT220930.5523250000000001Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCT198620.49655000000000005Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG179590.44897499999999996TruSeq Adapter, Index 8 (100% over 49bp)
TTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTCTG176560.4414Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCTGA175820.43955Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGTC175790.439475No Hit
AGCCCGCGGACGGTGTGAGGCCGGTGTCGGCCAGATCGGAAGAGCACACG162300.40575000000000006No Hit
TTGAATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCT161360.40340000000000004Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTGAACCCCGCCCGTGATGGGGACCGGGGATTAGATCGGAAGAGCACAC161180.40295000000000003No Hit
GAGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTG149600.374Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGTC148820.37205No Hit
CGCCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTC147240.3681No Hit
CCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCTG146030.365075Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGAATGAGTGCATCCCAAACCCATGGGCGAAGATCGGAAGAGCACACGT137010.34252499999999997No Hit
CGCCGAGAAGACGATCGAACTCGGTCGTAGATCGGAAGAGCACACGTCTG114880.2872Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCTGAA111660.27915Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTCTG107960.26990000000000003Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CATTGAATACCCCAGCTAGGAATAATGAGATCGGAAGAGCACACGTCTGA106460.26615Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGTCT101980.25495Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGATTAATAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGA97120.24280000000000002Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGAGAAGACGATCGAACTCGGTCGTTTAAGATCGGAAGAGCACACGTC93960.2349No Hit
CCCATTGAATACCCCAGCTAGGAATAATGAGATCGGAAGAGCACACGTCT93230.233075Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGATTAATAGTCCTACGTGATCTGAGAGATCGGAAGAGCACACGTCTGA91810.229525Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGT90420.22605No Hit
AATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTCTGAAC90320.2258Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGGCCTACGTCCTCCTAAGCGCAGACCGAGATCGGAAGAGCACACGTCT90130.225325Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGAATGAGTGCATCCCAAACCCATGGGCGAAGATCGGAAGAGCACACGTC87200.218No Hit
AGTGCGGTGACGCAAACGAACCCGGAGAAGATCGGAAGAGCACACGTCTG85230.213075Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAACGATGCCGACCCGCGATCCGGCGGCGAGATCGGAAGAGCACACGTCT84340.21085Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGCCCCATTGAATACCCCAGCTAGGAATAAGATCGGAAGAGCACACGTC80950.202375No Hit
ATTGAATACCCCAGCTAGGAATAATGGAAGATCGGAAGAGCACACGTCTG77460.19365000000000002Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACGATTAATAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCT72780.18195Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACG71950.179875No Hit
TTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCTGAA68040.1701Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGAGATCGGAAGAGCACAC66910.167275No Hit
ACTGTGGCAATTCCAGAGCTAATACATGCAGATCGGAAGAGCACACGTCT65810.164525Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCCGCGGACGGTGTGAGGCCGGTGTCGAGATCGGAAGAGCACACGTCT65170.16292500000000001Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGCCTGGGAATACCAGGTGCTGTAAGAGATCGGAAGAGCACACGTCTG62170.155425Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGAGATCGGAAGAGCACACG62130.155325No Hit
CAGTGCGGTGACGCAAACGAACCCGGAGAAGATCGGAAGAGCACACGTCT61240.1531Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGGCGCAATGAAGGTGAGAACGGGGGAGATCGGAAGAGCACACGTCTGA60570.151425Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTTTCCTCACTTACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCT60230.150575Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTGAACCCCGCCCGTGATGGGGACCGGGGATAGATCGGAAGAGCACACG58890.147225No Hit
GAGCGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGT56120.1403No Hit
CCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGTCT55870.139675Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTGCGGTGACGCAAACGAACCCGGAGATAGATCGGAAGAGCACACGTCT55320.13829999999999998Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCATTGAATACCCCAGCTAGGAATAATGGAAGATCGGAAGAGCACACGTC55250.138125No Hit
TGAATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCTG53610.134025Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGAGTCGGGTCCAGATCCCCGAGCCCGGAGAGATCGGAAGAGCACACG51840.1296No Hit
CCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCTGA50070.125175Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACTGTGGCAATTCCAGAGCTAATACATGCAGATCGGAAGAGCACACGTC49640.12409999999999999No Hit
CCGAGAAGACGATCGAACTCGGTCGTTTAAGATCGGAAGAGCACACGTCT48940.12235Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGTCT48760.12190000000000001Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGAGAAGACGATCGAACTCGGTCGTTTAAGATCGGAAGAGCACACGT48570.121425No Hit
CAGTGCGGTGACGCAAACGAACCCGGAGAGATCGGAAGAGCACACGTCTG47280.11820000000000001Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCCATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGT45380.11345000000000001No Hit
CTGTGGCAATTCCAGAGCTAATACATGCAGATCGGAAGAGCACACGTCTG43960.1099Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTGCGGTGACGCAAACGAACCCGGAGATGAGATCGGAAGAGCACACGTC43530.108825No Hit
TGCGGCGCAATGAAGGTGAGAACGGGGGAGATCGGAAGAGCACACGTCTG41650.10412500000000001Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)