FastQCFastQC Report
Wed 27 Sept 2023
SRR1062398.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1062398.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences246773
Total Bases18.7 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTC120224.871683693110673No Hit
CATTGAATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGT100144.057980411147087No Hit
CATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCTG56912.3061680167603424Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGT44031.7842308518354926No Hit
CCATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGT41171.668334866456217No Hit
ACGATTAATAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTG32361.3113266038018747Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTCT28551.1569337002022102Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGT28501.1549075466116634No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGTC27521.1151949362369467No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACG26631.0791294023252138No Hit
GGATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTC25371.028070331843435No Hit
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGT25211.0215866403536853No Hit
CATTGAATACCCCAGCTAGGAATAATGGAAGATCGGAAGAGCACACGTCT24100.9766060306435469Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACG24010.9729589541805627No Hit
ATTGAATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTC23700.9603968019191728No Hit
CCCATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTC18080.7326571383417149No Hit
GCCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCT17680.7164479096173406Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCTG13990.5669177746349884Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCT13490.5466562387295206Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGA12560.5089697819453506Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCTGA11960.4846559388587892Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTCTG11850.4801984009595864Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGAATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCT11430.46317871079899336Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGTC10370.4202242546794017No Hit
GTTGAACCCCGCCCGTGATGGGGACCGGGGATTAGATCGGAAGAGCACAC10280.4165771782164175No Hit
CGCCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCT10230.4145510246258708Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG9490.38456395148577843TruSeq Adapter, Index 8 (100% over 49bp)
TGGAATGAGTGCATCCCAAACCCATGGGCGAAGATCGGAAGAGCACACGT9200.37281226066060713No Hit
CCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCTG8860.359034416244889Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCCGCGGACGGTGTGAGGCCGGTGTCGGCCAGATCGGAAGAGCACACG8660.3509298018827019No Hit
GAGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTG8660.3509298018827019Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGTC8570.3472827254197177No Hit
CGCCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTC7880.31932180587017217No Hit
CATTGAATACCCCAGCTAGGAATAATGAGATCGGAAGAGCACACGTCTGA7210.2921713477568454Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCTGAA7120.28852427129386116Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTCTG6650.26947842754272144Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGATTAATAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGA6360.25772673671755014Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGAGAAGACGATCGAACTCGGTCGTAGATCGGAAGAGCACACGTCTG6240.25286396810023787Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTCTGAAC5930.24030181583884783Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCCATTGAATACCCCAGCTAGGAATAATGAGATCGGAAGAGCACACGTCT5720.2317919707585514Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGATTAATAGTCCTACGTGATCTGAGAGATCGGAAGAGCACACGTCTGA5630.22814489429556717Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGT5580.2261187407050204No Hit
GGAATGAGTGCATCCCAAACCCATGGGCGAAGATCGGAAGAGCACACGTC5550.22490304855069232No Hit
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGTCT5430.22004027993338007Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACGATGCCGACCCGCGATCCGGCGGCGAGATCGGAAGAGCACACGTCT5340.21639320347039587Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGAGAAGACGATCGAACTCGGTCGTTTAAGATCGGAAGAGCACACGTC5320.21558274203417718No Hit
CGGGCCTACGTCCTCCTAAGCGCAGACCGAGATCGGAAGAGCACACGTCT5250.2127461270074117Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTGAATACCCCAGCTAGGAATAATGGAAGATCGGAAGAGCACACGTCTG5150.2086938198263181Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTGCGGTGACGCAAACGAACCCGGAGAAGATCGGAAGAGCACACGTCTG5150.2086938198263181Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCTGAA4830.19572643684681873Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGCCCCATTGAATACCCCAGCTAGGAATAAGATCGGAAGAGCACACGTC4750.19248459110194388No Hit
AACGATTAATAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCT4720.1912688989476158Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTGTGGCAATTCCAGAGCTAATACATGCAGATCGGAAGAGCACACGTCT4390.1778962852500071Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACG4240.17181782447836677No Hit
AACTGTGGCAATTCCAGAGCTAATACATGCAGATCGGAAGAGCACACGTC3920.15885044149886737No Hit
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGAGATCGGAAGAGCACACG3800.15398767288155513No Hit
GAGCGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGT3710.15034059641857092No Hit
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGAGATCGGAAGAGCACAC3670.1487196735461335No Hit
CAGTGCGGTGACGCAAACGAACCCGGAGAAGATCGGAAGAGCACACGTCT3590.14547782780125865Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGCCTGGGAATACCAGGTGCTGTAAGAGATCGGAAGAGCACACGTCTG3550.14385690492882122Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCATTGAATACCCCAGCTAGGAATAATGGAAGATCGGAAGAGCACACGTC3480.14102028990205573No Hit
TGAATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCTG3480.14102028990205573Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTGCGGTGACGCAAACGAACCCGGAGATAGATCGGAAGAGCACACGTCT3420.13858890559339962Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTTTCCTCACTTACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCT3400.13777844415718088Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGGCGCAATGAAGGTGAGAACGGGGGAGATCGGAAGAGCACACGTCTGA3360.13615752128474345Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGAACCCCGCCCGTGATGGGGACCGGGGATAGATCGGAAGAGCACACG3330.13494182913041539No Hit
CAGTGCGGTGACGCAAACGAACCCGGAGAGATCGGAAGAGCACACGTCTG3320.13453659841230606Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCCGCGGACGGTGTGAGGCCGGTGTCGAGATCGGAAGAGCACACGTCT3070.12440583045957217Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGTCT3020.12237967686902539Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGAGTCGGGTCCAGATCCCCGAGCCCGGAGAGATCGGAAGAGCACACG2990.1211639847146973No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGTCT2950.11954306184225989Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGAGAAGACGATCGAACTCGGTCGTTTAAGATCGGAAGAGCACACGT2950.11954306184225989No Hit
CCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCTGA2880.1167064468154944Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTGCGGTGACGCAAACGAACCCGGAGATGAGATCGGAAGAGCACACGTC2880.1167064468154944No Hit
CCGAGAAGACGATCGAACTCGGTCGTTTAAGATCGGAAGAGCACACGTCT2880.1167064468154944Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGTGGCAATTCCAGAGCTAATACATGCAGATCGGAAGAGCACACGTCTG2820.11427506250683828Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTTCCTCACTTACCCGGTTGGGCAGGGAGATCGGAAGAGCACACGTCTG2660.10779137101708859Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGATTAATAGTCCTACGTGATCTGAGTTAGATCGGAAGAGCACACGTCT2620.10617044814465115Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTAAATGACTCGTAGACGACCTGATTAGATCGGAAGAGCACACGTCTGAA2480.10049721809112018Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)