FastQCFastQC Report
Wed 27 Sept 2023
SRR1062400.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1062400.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Total Bases304 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTC1817404.5435No Hit
CATTGAATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGT1489833.724575No Hit
CATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCTG868702.1717500000000003Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGT792771.981925No Hit
CCATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGT626031.5650750000000002No Hit
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGT532911.3322749999999999No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGTC518731.2968250000000001No Hit
ACGATTAATAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTG497081.2427000000000001Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGT494251.235625No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACG458991.147475No Hit
ATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTCT452521.1313Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACG429041.0726No Hit
GGATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTC419451.0486250000000001No Hit
CATTGAATACCCCAGCTAGGAATAATGGAAGATCGGAAGAGCACACGTCT363540.9088499999999999Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTGAATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTC353630.8840749999999999No Hit
CCCATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTC331990.829975No Hit
GCCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCT326080.8151999999999999Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCTG269190.672975Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGA240840.6021000000000001Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCT218410.546025Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCT217610.544025Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG195260.48815000000000003TruSeq Adapter, Index 8 (100% over 49bp)
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGTC192360.4809No Hit
AGCCCGCGGACGGTGTGAGGCCGGTGTCGGCCAGATCGGAAGAGCACACG184750.461875No Hit
ATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCTGA183460.45865Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTCTG171390.42847500000000005Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGTC169960.4249No Hit
GTTGAACCCCGCCCGTGATGGGGACCGGGGATTAGATCGGAAGAGCACAC166950.41737500000000005No Hit
GAGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTG160980.40245000000000003Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTC160190.40047499999999997No Hit
TTGAATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCT154780.38695Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCTG153570.383925Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGAATGAGTGCATCCCAAACCCATGGGCGAAGATCGGAAGAGCACACGT135410.33852499999999996No Hit
CGCCGAGAAGACGATCGAACTCGGTCGTAGATCGGAAGAGCACACGTCTG123770.309425Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCTGAA115580.28895000000000004Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGTCT114240.2856Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CATTGAATACCCCAGCTAGGAATAATGAGATCGGAAGAGCACACGTCTGA107600.269Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTCTG107090.267725Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCATTGAATACCCCAGCTAGGAATAATGAGATCGGAAGAGCACACGTCT102260.25565000000000004Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGAGAAGACGATCGAACTCGGTCGTTTAAGATCGGAAGAGCACACGTC97170.242925No Hit
CGATTAATAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGA95720.2393Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGT93970.23492500000000002No Hit
ACGATTAATAGTCCTACGTGATCTGAGAGATCGGAAGAGCACACGTCTGA92180.23045000000000002Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTCTGAAC91000.2275Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGGCCTACGTCCTCCTAAGCGCAGACCGAGATCGGAAGAGCACACGTCT89300.22325Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACGATGCCGACCCGCGATCCGGCGGCGAGATCGGAAGAGCACACGTCT88250.220625Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGCCCCATTGAATACCCCAGCTAGGAATAAGATCGGAAGAGCACACGTC86850.217125No Hit
AGTGCGGTGACGCAAACGAACCCGGAGAAGATCGGAAGAGCACACGTCTG84610.211525Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGAATGAGTGCATCCCAAACCCATGGGCGAAGATCGGAAGAGCACACGTC83450.208625No Hit
ATTGAATACCCCAGCTAGGAATAATGGAAGATCGGAAGAGCACACGTCTG78000.19499999999999998Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGAGATCGGAAGAGCACAC77130.192825No Hit
AGCCCGCGGACGGTGTGAGGCCGGTGTCGAGATCGGAAGAGCACACGTCT76370.19092499999999998Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACG76150.19037500000000002No Hit
AACGATTAATAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCT69710.17427499999999999Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGAGATCGGAAGAGCACACG68910.172275No Hit
TTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCTGAA66580.16645Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGTTTCCTCACTTACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCT65180.16295Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGCCTGGGAATACCAGGTGCTGTAAGAGATCGGAAGAGCACACGTCTG64530.161325Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGGCGCAATGAAGGTGAGAACGGGGGAGATCGGAAGAGCACACGTCTGA64140.16035000000000002Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGTGCGGTGACGCAAACGAACCCGGAGAAGATCGGAAGAGCACACGTCT63130.157825Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTGTGGCAATTCCAGAGCTAATACATGCAGATCGGAAGAGCACACGTCT62810.157025Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGCGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGT61630.154075No Hit
GTTGAACCCCGCCCGTGATGGGGACCGGGGATAGATCGGAAGAGCACACG60170.150425No Hit
CCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGTCT59540.14885Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCATTGAATACCCCAGCTAGGAATAATGGAAGATCGGAAGAGCACACGTC55410.138525No Hit
AGTGCGGTGACGCAAACGAACCCGGAGATAGATCGGAAGAGCACACGTCT54850.137125Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGAGAAGACGATCGAACTCGGTCGTTTAAGATCGGAAGAGCACACGT54800.13699999999999998No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGTCT53910.134775Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCCATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGT53480.1337No Hit
TGAATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCTG51240.1281Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCTGA50880.12719999999999998Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGGAGTCGGGTCCAGATCCCCGAGCCCGGAGAGATCGGAAGAGCACACG50790.126975No Hit
CCGAGAAGACGATCGAACTCGGTCGTTTAAGATCGGAAGAGCACACGTCT49900.12474999999999999Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACTGTGGCAATTCCAGAGCTAATACATGCAGATCGGAAGAGCACACGTC47680.11919999999999999No Hit
CAGTGCGGTGACGCAAACGAACCCGGAGAGATCGGAAGAGCACACGTCTG46660.11665Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCCGCGGACGGTGTGAGGCCGGTGTCAGATCGGAAGAGCACACGTCTG44110.110275Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTGCGGTGACGCAAACGAACCCGGAGATGAGATCGGAAGAGCACACGTC43970.109925No Hit
AGCCCGCGGACGGTGTGAGGCCGGTGTCGGAGATCGGAAGAGCACACGTC43690.10922499999999999No Hit
TCCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGTC42530.106325No Hit
TGCGGCGCAATGAAGGTGAGAACGGGGGAGATCGGAAGAGCACACGTCTG42240.10560000000000001Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCCGGGGCCGCGGGTGAGTGTCCTCCGCGCAGATCGGAAGAGCACACGT42190.10547499999999999No Hit
GCCGAGAAGACGATCGAACTCGGTCGTAGATCGGAAGAGCACACGTCTGA41260.10315Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCTTGCTCTCCGGGGCCGCGGGTGAGTGAGATCGGAAGAGCACACGTCT40940.10235000000000001Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGTGGCAATTCCAGAGCTAATACATGCAGATCGGAAGAGCACACGTCTG40590.10147500000000001Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)