FastQCFastQC Report
Wed 27 Sept 2023
SRR1062423.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1062423.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Total Bases304 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTC1823364.5584No Hit
CATTGAATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGT1496423.74105No Hit
CATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCTG870572.176425Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGT778501.94625No Hit
CCATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGT628191.570475No Hit
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGT519141.29785No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGTC506701.26675No Hit
ACGATTAATAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTG499021.24755Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGT486871.2171750000000001No Hit
ATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTCT454491.136225Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACG447101.11775No Hit
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACG421821.0545499999999999No Hit
GGATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTC418421.04605No Hit
CATTGAATACCCCAGCTAGGAATAATGGAAGATCGGAAGAGCACACGTCT364800.9119999999999999Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTGAATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTC351140.87785No Hit
CCCATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTC329590.823975No Hit
GCCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCT325200.8130000000000001Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCTG265220.66305Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGA236290.5907250000000001Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCT220810.552025Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCT213570.533925Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG193150.48287499999999994TruSeq Adapter, Index 8 (100% over 49bp)
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGTC189510.473775No Hit
ATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCTGA181150.45287499999999997Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCCGCGGACGGTGTGAGGCCGGTGTCGGCCAGATCGGAAGAGCACACG179820.44955000000000006No Hit
TTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTCTG170620.42655000000000004Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGTC166900.41725No Hit
GTTGAACCCCGCCCGTGATGGGGACCGGGGATTAGATCGGAAGAGCACAC163480.40870000000000006No Hit
GAGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTG156700.39175Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTC156420.39105No Hit
TTGAATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCT153580.38395Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCTG149920.3748Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGAATGAGTGCATCCCAAACCCATGGGCGAAGATCGGAAGAGCACACGT133980.33495No Hit
CGCCGAGAAGACGATCGAACTCGGTCGTAGATCGGAAGAGCACACGTCTG122960.3074Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGTCT113670.284175Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCTGAA111930.279825Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CATTGAATACCCCAGCTAGGAATAATGAGATCGGAAGAGCACACGTCTGA107440.2686Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTCTG106930.267325Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCATTGAATACCCCAGCTAGGAATAATGAGATCGGAAGAGCACACGTCT99460.24865Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGAGAAGACGATCGAACTCGGTCGTTTAAGATCGGAAGAGCACACGTC96990.242475No Hit
CGATTAATAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGA95880.23969999999999997Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAACGATGCCGACCCGCGATCCGGCGGCGAGATCGGAAGAGCACACGTCT92140.23035Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGT91970.22992500000000002No Hit
AATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTCTGAAC91790.22947499999999998Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGGCCTACGTCCTCCTAAGCGCAGACCGAGATCGGAAGAGCACACGTCT90080.2252Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGATTAATAGTCCTACGTGATCTGAGAGATCGGAAGAGCACACGTCTGA89420.22355Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTGCGGTGACGCAAACGAACCCGGAGAAGATCGGAAGAGCACACGTCTG85670.214175Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGAATGAGTGCATCCCAAACCCATGGGCGAAGATCGGAAGAGCACACGTC84810.21202500000000002No Hit
GCGCCCCATTGAATACCCCAGCTAGGAATAAGATCGGAAGAGCACACGTC84510.211275No Hit
ATTGAATACCCCAGCTAGGAATAATGGAAGATCGGAAGAGCACACGTCTG77910.194775Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACG77370.19342499999999999No Hit
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGAGATCGGAAGAGCACAC74870.18717499999999998No Hit
AGCCCGCGGACGGTGTGAGGCCGGTGTCGAGATCGGAAGAGCACACGTCT72790.181975Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACGATTAATAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCT68780.17195000000000002Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGAGATCGGAAGAGCACACG68000.16999999999999998No Hit
TTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCTGAA67110.167775Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCGCCTGGGAATACCAGGTGCTGTAAGAGATCGGAAGAGCACACGTCTG66110.165275Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGGCGCAATGAAGGTGAGAACGGGGGAGATCGGAAGAGCACACGTCTGA65010.162525Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTTTCCTCACTTACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCT63840.1596Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTGTGGCAATTCCAGAGCTAATACATGCAGATCGGAAGAGCACACGTCT62450.15612499999999999Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAGTGCGGTGACGCAAACGAACCCGGAGAAGATCGGAAGAGCACACGTCT62000.155Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGTCT60580.15145Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGCGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGT60530.15132500000000002No Hit
GTTGAACCCCGCCCGTGATGGGGACCGGGGATAGATCGGAAGAGCACACG59050.14762499999999998No Hit
CCATTGAATACCCCAGCTAGGAATAATGGAAGATCGGAAGAGCACACGTC55500.13875No Hit
AGTGCGGTGACGCAAACGAACCCGGAGATAGATCGGAAGAGCACACGTCT54830.137075Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGAGAAGACGATCGAACTCGGTCGTTTAAGATCGGAAGAGCACACGT54250.135625No Hit
CCCCATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGT52960.13240000000000002No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGTCT52780.13194999999999998Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCTGA50680.12669999999999998Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGAGAAGACGATCGAACTCGGTCGTTTAAGATCGGAAGAGCACACGTCT50370.125925Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGAGTCGGGTCCAGATCCCCGAGCCCGGAGAGATCGGAAGAGCACACG50200.1255No Hit
TGAATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCTG49790.124475Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGTGCGGTGACGCAAACGAACCCGGAGAGATCGGAAGAGCACACGTCTG49380.12344999999999999Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACTGTGGCAATTCCAGAGCTAATACATGCAGATCGGAAGAGCACACGTC47030.117575No Hit
AGTGCGGTGACGCAAACGAACCCGGAGATGAGATCGGAAGAGCACACGTC44230.11057499999999999No Hit
AGCCCGCGGACGGTGTGAGGCCGGTGTCGGAGATCGGAAGAGCACACGTC43740.10935No Hit
CTGTGGCAATTCCAGAGCTAATACATGCAGATCGGAAGAGCACACGTCTG41870.10467499999999999Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCCGGGGCCGCGGGTGAGTGTCCTCCGCGCAGATCGGAAGAGCACACGT41740.10435No Hit
AGCCCGCGGACGGTGTGAGGCCGGTGTCAGATCGGAAGAGCACACGTCTG41730.10432499999999999Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCGGCGCAATGAAGGTGAGAACGGGGGAGATCGGAAGAGCACACGTCTG41380.10345Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCGAGAAGACGATCGAACTCGGTCGTAGATCGGAAGAGCACACGTCTGA40970.102425Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGTC40760.10189999999999999No Hit
CGCTTGCTCTCCGGGGCCGCGGGTGAGTGAGATCGGAAGAGCACACGTCT40630.101575Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGATTAATAGTCCTACGTGATCTGAGTTAGATCGGAAGAGCACACGTCT40170.10042499999999999Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)