FastQCFastQC Report
Wed 27 Sept 2023
SRR1062435.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1062435.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Total Bases304 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTC1810084.5252No Hit
CATTGAATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGT1480433.7010750000000003No Hit
CATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCTG871262.17815Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGT799021.99755No Hit
CCATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGT622441.5561No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGTC532321.3308No Hit
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGT526041.3151No Hit
ACGATTAATAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTG506861.26715Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGT494941.2373500000000002No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACG466521.1663No Hit
ATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTCT450611.126525Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACG431541.0788499999999999No Hit
GGATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTC421101.05275No Hit
CATTGAATACCCCAGCTAGGAATAATGGAAGATCGGAAGAGCACACGTCT362150.9053749999999999Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTGAATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTC353640.8841No Hit
CCCATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTC328630.821575No Hit
GCCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCT327770.8194250000000001Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCTG270440.6760999999999999Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGA245860.61465Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCT220870.552175Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCT216570.5414249999999999Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG200710.501775TruSeq Adapter, Index 8 (100% over 49bp)
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGTC194320.4858No Hit
AGCCCGCGGACGGTGTGAGGCCGGTGTCGGCCAGATCGGAAGAGCACACG184310.460775No Hit
ATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCTGA181860.45465Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGTC176380.44095No Hit
TTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTCTG172960.43239999999999995Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTGAACCCCGCCCGTGATGGGGACCGGGGATTAGATCGGAAGAGCACAC165120.41279999999999994No Hit
GAGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTG161350.403375Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTC157660.39414999999999994No Hit
CCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCTG155180.38795Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGAATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCT153940.38484999999999997Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGAATGAGTGCATCCCAAACCCATGGGCGAAGATCGGAAGAGCACACGT135210.338025No Hit
CGCCGAGAAGACGATCGAACTCGGTCGTAGATCGGAAGAGCACACGTCTG122620.30655Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGTCT114850.287125Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCTGAA112710.281775Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTCTG107740.26935000000000003Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CATTGAATACCCCAGCTAGGAATAATGAGATCGGAAGAGCACACGTCTGA107560.2689Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCATTGAATACCCCAGCTAGGAATAATGAGATCGGAAGAGCACACGTCT103910.259775Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGAGAAGACGATCGAACTCGGTCGTTTAAGATCGGAAGAGCACACGTC96680.2417No Hit
CGATTAATAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGA95980.23995000000000002Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGT94720.23679999999999998No Hit
CGGGCCTACGTCCTCCTAAGCGCAGACCGAGATCGGAAGAGCACACGTCT92820.23205Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACGATGCCGACCCGCGATCCGGCGGCGAGATCGGAAGAGCACACGTCT92330.230825Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGATTAATAGTCCTACGTGATCTGAGAGATCGGAAGAGCACACGTCTGA92220.23054999999999998Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTCTGAAC91740.22935Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCGCCCCATTGAATACCCCAGCTAGGAATAAGATCGGAAGAGCACACGTC88500.22125No Hit
AGTGCGGTGACGCAAACGAACCCGGAGAAGATCGGAAGAGCACACGTCTG84310.210775Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGAATGAGTGCATCCCAAACCCATGGGCGAAGATCGGAAGAGCACACGTC83840.2096No Hit
GAGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACG79220.19805No Hit
ATTGAATACCCCAGCTAGGAATAATGGAAGATCGGAAGAGCACACGTCTG78620.19654999999999997Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCCGCGGACGGTGTGAGGCCGGTGTCGAGATCGGAAGAGCACACGTCT77070.192675Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGAGATCGGAAGAGCACAC76710.191775No Hit
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGAGATCGGAAGAGCACACG70190.175475No Hit
AACGATTAATAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCT67920.1698Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCTGAA66140.16535Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCGCCTGGGAATACCAGGTGCTGTAAGAGATCGGAAGAGCACACGTCTG65180.16295Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTTTCCTCACTTACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCT65110.162775Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGGCGCAATGAAGGTGAGAACGGGGGAGATCGGAAGAGCACACGTCTGA64780.16195Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGTGCGGTGACGCAAACGAACCCGGAGAAGATCGGAAGAGCACACGTCT64050.160125Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTGTGGCAATTCCAGAGCTAATACATGCAGATCGGAAGAGCACACGTCT63770.15942499999999998Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGCGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGT61160.1529No Hit
CCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGTCT60900.15225Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTGAACCCCGCCCGTGATGGGGACCGGGGATAGATCGGAAGAGCACACG59990.149975No Hit
AGTGCGGTGACGCAAACGAACCCGGAGATAGATCGGAAGAGCACACGTCT55880.1397Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCATTGAATACCCCAGCTAGGAATAATGGAAGATCGGAAGAGCACACGTC55470.138675No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGTCT54860.13715000000000002Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCCATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGT52600.1315No Hit
CGCCGAGAAGACGATCGAACTCGGTCGTTTAAGATCGGAAGAGCACACGT52400.131No Hit
AGGGAGTCGGGTCCAGATCCCCGAGCCCGGAGAGATCGGAAGAGCACACG52150.130375No Hit
CCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCTGA51910.129775Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGAATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCTG51150.127875Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGAGAAGACGATCGAACTCGGTCGTTTAAGATCGGAAGAGCACACGTCT50880.12719999999999998Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAGTGCGGTGACGCAAACGAACCCGGAGAGATCGGAAGAGCACACGTCTG48270.12067499999999999Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACTGTGGCAATTCCAGAGCTAATACATGCAGATCGGAAGAGCACACGTC47860.11965No Hit
AGTGCGGTGACGCAAACGAACCCGGAGATGAGATCGGAAGAGCACACGTC44500.11125No Hit
AGCCCGCGGACGGTGTGAGGCCGGTGTCGGAGATCGGAAGAGCACACGTC43790.109475No Hit
AGCCCGCGGACGGTGTGAGGCCGGTGTCAGATCGGAAGAGCACACGTCTG42940.10735Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGTGGCAATTCCAGAGCTAATACATGCAGATCGGAAGAGCACACGTCTG42420.10605Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCGGCGCAATGAAGGTGAGAACGGGGGAGATCGGAAGAGCACACGTCTG41930.10482499999999999Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGTC41320.1033No Hit
GCCGAGAAGACGATCGAACTCGGTCGTAGATCGGAAGAGCACACGTCTGA41130.10282500000000001Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCCGGGGCCGCGGGTGAGTGTCCTCCGCGCAGATCGGAAGAGCACACGT41130.10282500000000001No Hit
CGCTTGCTCTCCGGGGCCGCGGGTGAGTGAGATCGGAAGAGCACACGTCT40160.10039999999999999Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)