FastQCFastQC Report
Wed 27 Sept 2023
SRR1062462.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1062462.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Total Bases304 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCGCCTGGGAATACCAGGTGCTGTAAGAGATCGGAAGAGCACACGTCTG1636714.091775Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG1591893.9797249999999997TruSeq Adapter, Index 10 (100% over 49bp)
ATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTCTG395840.9896Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGT350250.875625No Hit
CGGGCCTACGTCCTCCTAAGCGCAGACCGAGATCGGAAGAGCACACGTCT342830.857075Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGCCTGGGAATACCAGGTGCTGTAAAGATCGGAAGAGCACACGTCTGA280570.701425Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTCGGTCGGTGCGGAGAGCCAGCCGTGAGATCGGAAGAGCACACGTCTG237700.59425Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCTTTCGAACGCACATTGCGGCCCCGGGTAGATCGGAAGAGCACACGTC216520.5413No Hit
GGATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTC159060.39765No Hit
ACTTCGCCGCTTGCTCTCCGGGGCCGCGGGTAGATCGGAAGAGCACACGT153150.382875No Hit
AACTCTTAGCGGTGGATCACTCGGCTCGAGATCGGAAGAGCACACGTCTG150060.37515Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCTTTCGAACGCACATTGCGGCCCCGGGAGATCGGAAGAGCACACGTCT149330.373325Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGTC147200.368No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACG140510.351275No Hit
CGCCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCT137960.3449Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACACTTCGAACGCACTTTGCGGCCCCGGAGATCGGAAGAGCACACGTCTG122470.306175Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCTG121930.304825Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGTCT119810.299525Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACTCTTAGCGGTGGATCACTCGGAGATCGGAAGAGCACACGTCTGAACT117750.294375Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACG117320.2933No Hit
CCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGTCTG113740.28435Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACCGCCTGGGAATACCAGGTGCTGTAAGAGATCGGAAGAGCACACGTCT110740.27685000000000004Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGT104430.261075No Hit
AACTCTTAGCGGTGGATCACTCGGCTCGTGAGATCGGAAGAGCACACGTC101760.25439999999999996No Hit
CGGATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGT98870.247175No Hit
TACCTGGTTGATCCTGCCAGTAACAGATCGGAAGAGCACACGTCTGAACT98110.24527500000000002Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGA96510.241275Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTTTCGAACGCACATTGCGGCCCCGGGAGATCGGAAGAGCACACGTCTG96410.24102500000000002Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGT96130.240325No Hit
GGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGAAC92680.23170000000000002Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTC91070.22767500000000002No Hit
CGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGTCTG88540.22135000000000002Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGTCT88480.2212Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACGTC87430.21857500000000002No Hit
ACTTCGAACGCACTTTGCGGCCCCGGAGATCGGAAGAGCACACGTCTGAA85140.21285Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTCT82150.205375Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCCTGGGAATACCAGGTGCTGTAAGAGATCGGAAGAGCACACGTCTGA81340.20335Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGCGGGGGGCGCACCACCGGCCCGTCTCGTAGATCGGAAGAGCACACGT79890.19972499999999999No Hit
CCTCGGTCGGTGCGGAGAGCCAGCCGAGATCGGAAGAGCACACGTCTGAA77460.19365000000000002Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGAACTC75800.1895Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGTC75430.188575No Hit
CGGGGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGA72150.180375Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTC70690.176725No Hit
GAGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTG70290.175725Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGAACT66920.1673Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTGCGAGAACTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTGAA66160.16540000000000002Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGACCGCCTGGGAATACCAGGTGCTGTAAGAGATCGGAAGAGCACACGTC65980.16494999999999999No Hit
GCCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCT64020.16005Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCTCGGCGCGGGCCGGGGGGCCAAAAGATCGGAAGAGCACACGTCTGA63960.1599Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCTGA61950.154875Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGGGTCAGGGGGCGAAGGTGGCCCGTCGGAGATCGGAAGAGCACACGTC61370.153425No Hit
CCGAGAAGACGATCGAACTCGGTCGTTTAAGATCGGAAGAGCACACGTCT60780.15195Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTC57340.14335No Hit
CCTCGGTCGGTGCGGAGAGCCAGCCGTAGATCGGAAGAGCACACGTCTGA57110.14277499999999999Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGAACCCCGCCCGTGATGGGGACCGGGGATTAGATCGGAAGAGCACACG56800.14200000000000002No Hit
ACCGCCTGGGAATACCAGGTGCTGAGATCGGAAGAGCACACGTCTGAACT55630.139075Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCGCCTGGGAATACCAGGTGCTGTAAGATCGGAAGAGCACACGTCTGAA55100.13775Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGATTAATAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTG52880.1322Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTTCTACAGTCCGACGATCAAACAGATCGGAAGAGCACACGTCTGAACT51660.12915Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTCCTCACTTACCCGGTTGGGCAGGGAGATCGGAAGAGCACACGTCTGA50510.126275Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGAAACTGCGAATGGCTCATTAAATCAGAGATCGGAAGAGCACACGTCTG50330.125825Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGTCTGAA50120.1253Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTCTGA49880.1247Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAACTCTTAGCGGTGGATCACTCGGCTCGAGATCGGAAGAGCACACGTCT48450.12112500000000001Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCTTGCTACCCGGGGCCGCGGGCAAGATCGGAAGAGCACACGTCTGAA48080.1202Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTTGAACCCCGCCCGTGATGGGGACCGGGGATTAGATCGGAAGAGCACAC47110.11777499999999999No Hit
AACCCCGCCCGTGATGGGGACCGGGGATTAGATCGGAAGAGCACACGTCT45740.11435Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACGATGCCGACCCGCGATCCGGCGGCGAGATCGGAAGAGCACACGTCT45500.11375Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGTCT44990.112475Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGTC44310.110775No Hit
CATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCTG44030.11007499999999999Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACACTTCGAACGCACTTTGCGGCCCCGGGAGATCGGAAGAGCACACGTCT42890.107225Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGAGGGAAGAGCCCAGCGCCGAATCCCCAGATCGGAAGAGCACACGTCT42310.10577500000000001Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTTCGCCGCTTGCTCTCCGGGGCCGCGGGTGAGATCGGAAGAGCACACG42060.10515No Hit
GTTGAACCCCGCCCGTGATGGGGACCGGGGATAGATCGGAAGAGCACACG41900.10475000000000001No Hit
CTTCGCCGCTTGCTCTCCGGGGCCGCGGGTAGATCGGAAGAGCACACGTC41520.1038No Hit
ACTTCGAACGCACTTTGCGGCCCCGGGTAGATCGGAAGAGCACACGTCTG40740.10185000000000001Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)