FastQCFastQC Report
Thu 28 Sept 2023
SRR1062494.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1062494.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Total Bases304 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCGCCTGGGAATACCAGGTGCTGTAAGAGATCGGAAGAGCACACGTCTG1678734.196825Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG1623114.057775TruSeq Adapter, Index 10 (100% over 49bp)
ATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTCTG386040.9651Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGT378650.946625No Hit
CGGGCCTACGTCCTCCTAAGCGCAGACCGAGATCGGAAGAGCACACGTCT356380.8909500000000001Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGCCTGGGAATACCAGGTGCTGTAAAGATCGGAAGAGCACACGTCTGA288450.721125Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTCGGTCGGTGCGGAGAGCCAGCCGTGAGATCGGAAGAGCACACGTCTG241790.604475Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCTTTCGAACGCACATTGCGGCCCCGGGTAGATCGGAAGAGCACACGTC223500.55875No Hit
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGTC166280.4157No Hit
GGATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTC159670.39917499999999995No Hit
ACTTCGCCGCTTGCTCTCCGGGGCCGCGGGTAGATCGGAAGAGCACACGT153050.382625No Hit
AACTCTTAGCGGTGGATCACTCGGCTCGAGATCGGAAGAGCACACGTCTG152800.382Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCT151570.378925Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCTTTCGAACGCACATTGCGGCCCCGGGAGATCGGAAGAGCACACGTCT151320.37829999999999997Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACG147280.36819999999999997No Hit
CCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGTCT135430.33857499999999996Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGTCTG130290.32572500000000004Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACG126410.316025No Hit
CCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCTG120270.30067499999999997Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACTCTTAGCGGTGGATCACTCGGAGATCGGAAGAGCACACGTCTGAACT116090.290225Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACACTTCGAACGCACTTTGCGGCCCCGGAGATCGGAAGAGCACACGTCTG115390.28847500000000004Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACCGCCTGGGAATACCAGGTGCTGTAAGAGATCGGAAGAGCACACGTCT114920.2873Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGT112010.28002499999999997No Hit
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGTCT103640.2591Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACTCTTAGCGGTGGATCACTCGGCTCGTGAGATCGGAAGAGCACACGTC103400.2585No Hit
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGT102120.2553No Hit
AGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGA101300.25325Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGTCTG99330.24832500000000002Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTTTCGAACGCACATTGCGGCCCCGGGAGATCGGAAGAGCACACGTCTG99090.24772499999999997Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTC98320.24580000000000002No Hit
GGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGAAC97530.243825Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGT97320.2433No Hit
CCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACGTC94670.236675No Hit
TACCTGGTTGATCCTGCCAGTAACAGATCGGAAGAGCACACGTCTGAACT92080.2302Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGCCTGGGAATACCAGGTGCTGTAAGAGATCGGAAGAGCACACGTCTGA87070.21767499999999998Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGCGGGGGGCGCACCACCGGCCCGTCTCGTAGATCGGAAGAGCACACGT84860.21215No Hit
ACTTCGAACGCACTTTGCGGCCCCGGAGATCGGAAGAGCACACGTCTGAA82440.20609999999999998Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGTC81380.20345No Hit
CCTCGGTCGGTGCGGAGAGCCAGCCGAGATCGGAAGAGCACACGTCTGAA80630.201575Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTCT80200.20049999999999998Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGAACTC79680.1992Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTG74040.1851Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGA73860.18465Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTC70510.176275No Hit
GACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGAACT69770.174425Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCT68180.17045000000000002Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGACCGCCTGGGAATACCAGGTGCTGTAAGAGATCGGAAGAGCACACGTC66880.16720000000000002No Hit
CTGCGAGAACTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTGAA65830.164575Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTCTCGGCGCGGGCCGGGGGGCCAAAAGATCGGAAGAGCACACGTCTGA64320.1608Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGGGTCAGGGGGCGAAGGTGGCCCGTCGGAGATCGGAAGAGCACACGTC61970.154925No Hit
CCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCTGA61670.154175Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTCGGTCGGTGCGGAGAGCCAGCCGTAGATCGGAAGAGCACACGTCTGA60920.1523Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGAGAAGACGATCGAACTCGGTCGTTTAAGATCGGAAGAGCACACGTCT60180.15045Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGCCTGGGAATACCAGGTGCTGAGATCGGAAGAGCACACGTCTGAACT57680.1442Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCGCCTGGGAATACCAGGTGCTGTAAGATCGGAAGAGCACACGTCTGAA57110.14277499999999999Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGAACCCCGCCCGTGATGGGGACCGGGGATTAGATCGGAAGAGCACACG56740.14185No Hit
CATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTC54260.13565No Hit
CGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGTCTGAA53500.13374999999999998Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGCTTGCTACCCGGGGCCGCGGGCAAGATCGGAAGAGCACACGTCTGAA52750.131875Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGATTAATAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTG51150.127875Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTTCTACAGTCCGACGATCAAACAGATCGGAAGAGCACACGTCTGAACT49790.124475Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGTC48740.12185No Hit
CAACTCTTAGCGGTGGATCACTCGGCTCGAGATCGGAAGAGCACACGTCT48250.12062500000000001Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACCCCGCCCGTGATGGGGACCGGGGATTAGATCGGAAGAGCACACGTCT48070.12017499999999999Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGTCT47930.11982500000000001Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTGAACCCCGCCCGTGATGGGGACCGGGGATTAGATCGGAAGAGCACAC47420.11854999999999999No Hit
TCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTCTGA47320.1183Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTCCTCACTTACCCGGTTGGGCAGGGAGATCGGAAGAGCACACGTCTGA46090.11522500000000001Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGAAACTGCGAATGGCTCATTAAATCAGAGATCGGAAGAGCACACGTCTG45960.1149Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTGAACCCCGCCCGTGATGGGGACCGGGGATAGATCGGAAGAGCACACG45400.11349999999999999No Hit
CTTCGCCGCTTGCTCTCCGGGGCCGCGGGTAGATCGGAAGAGCACACGTC44670.111675No Hit
AAACGATGCCGACCCGCGATCCGGCGGCGAGATCGGAAGAGCACACGTCT44150.11037499999999999Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTTCGCCGCTTGCTCTCCGGGGCCGCGGGTGAGATCGGAAGAGCACACG43400.1085No Hit
CGCCCCCTCGCCCCGACTTCGCCGCTTGCAGATCGGAAGAGCACACGTCT42200.1055Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGTC42080.1052No Hit
CATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCTG41690.104225Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGTTCGGGGGCGAAGGTGGCTCGCGGCTAGATCGGAAGAGCACACGTCTG40940.10235000000000001Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGGGGGGCGCACCACCGGCCCGTCTCGTAGATCGGAAGAGCACACGTCT40930.10232500000000001Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCCTACGTCCTCCTAAGCGCAGACCGAGATCGGAAGAGCACACGTCTGA40670.101675Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCTTTCGAACGCACATTGCGGCCCCGGAGATCGGAAGAGCACACGTCTG40580.10145Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGAGGGAAGAGCCCAGCGCCGAATCCCCAGATCGGAAGAGCACACGTCT40370.100925Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)