FastQCFastQC Report
Thu 28 Sept 2023
SRR1062515.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1062515.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Total Bases304 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCGCCTGGGAATACCAGGTGCTGTAAGAGATCGGAAGAGCACACGTCTG1679814.1995249999999995Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG1629534.073824999999999TruSeq Adapter, Index 10 (100% over 49bp)
ATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTCTG391830.9795750000000001Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGT369270.9231750000000001No Hit
CGGGCCTACGTCCTCCTAAGCGCAGACCGAGATCGGAAGAGCACACGTCT351050.8776249999999999Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGCCTGGGAATACCAGGTGCTGTAAAGATCGGAAGAGCACACGTCTGA288420.72105Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTCGGTCGGTGCGGAGAGCCAGCCGTGAGATCGGAAGAGCACACGTCTG237710.594275Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCTTTCGAACGCACATTGCGGCCCCGGGTAGATCGGAAGAGCACACGTC220710.551775No Hit
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGTC161300.40324999999999994No Hit
GGATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTC157690.39422499999999994No Hit
AACTCTTAGCGGTGGATCACTCGGCTCGAGATCGGAAGAGCACACGTCTG153490.383725Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTTCGCCGCTTGCTCTCCGGGGCCGCGGGTAGATCGGAAGAGCACACGT153240.3831No Hit
ACCTTTCGAACGCACATTGCGGCCCCGGGAGATCGGAAGAGCACACGTCT152290.380725Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCT150050.375125Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACG146130.36532499999999996No Hit
CCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGTCT132740.33185Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACG127530.318825No Hit
CCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGTCTG124550.311375Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCTG119960.2999Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACACTTCGAACGCACTTTGCGGCCCCGGAGATCGGAAGAGCACACGTCTG116300.29075Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACTCTTAGCGGTGGATCACTCGGAGATCGGAAGAGCACACGTCTGAACT115530.288825Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GACCGCCTGGGAATACCAGGTGCTGTAAGAGATCGGAAGAGCACACGTCT114190.28547500000000003Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGT108060.27015No Hit
AACTCTTAGCGGTGGATCACTCGGCTCGTGAGATCGGAAGAGCACACGTC106060.26515No Hit
AGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGA100940.25235Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGT100630.251575No Hit
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGTCT98780.24695Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTTTCGAACGCACATTGCGGCCCCGGGAGATCGGAAGAGCACACGTCTG97510.243775Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGT97220.24305No Hit
GGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGAAC96390.240975Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TACCTGGTTGATCCTGCCAGTAACAGATCGGAAGAGCACACGTCTGAACT95890.23972500000000002Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTC95330.238325No Hit
CGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGTCTG94910.23727499999999999Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACGTC91820.22954999999999998No Hit
CCGCCTGGGAATACCAGGTGCTGTAAGAGATCGGAAGAGCACACGTCTGA83540.20885Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTCT81360.20339999999999997Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGCGGGGGGCGCACCACCGGCCCGTCTCGTAGATCGGAAGAGCACACGT81250.203125No Hit
ACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGAACTC81140.20285Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGTC80650.20162499999999997No Hit
ACTTCGAACGCACTTTGCGGCCCCGGAGATCGGAAGAGCACACGTCTGAA79420.19854999999999998Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCTCGGTCGGTGCGGAGAGCCAGCCGAGATCGGAAGAGCACACGTCTGAA77300.19325Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGGGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGA72600.1815Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTG71170.177925Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTC70210.17552500000000001No Hit
GACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGAACT68990.172475Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGACCGCCTGGGAATACCAGGTGCTGTAAGAGATCGGAAGAGCACACGTC66760.1669No Hit
GCCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCT66650.166625Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGCGAGAACTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTGAA63380.15845Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTCTCGGCGCGGGCCGGGGGGCCAAAAGATCGGAAGAGCACACGTCTGA63360.15839999999999999Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGGGTCAGGGGGCGAAGGTGGCCCGTCGGAGATCGGAAGAGCACACGTC62610.156525No Hit
CCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCTGA60650.151625Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTCGGTCGGTGCGGAGAGCCAGCCGTAGATCGGAAGAGCACACGTCTGA60180.15045Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGAGAAGACGATCGAACTCGGTCGTTTAAGATCGGAAGAGCACACGTCT58950.147375Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGCCTGGGAATACCAGGTGCTGTAAGATCGGAAGAGCACACGTCTGAA57060.14265Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCGCCTGGGAATACCAGGTGCTGAGATCGGAAGAGCACACGTCTGAACT57040.1426Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTC56760.1419No Hit
TTGAACCCCGCCCGTGATGGGGACCGGGGATTAGATCGGAAGAGCACACG56310.14077499999999998No Hit
CGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGTCTGAA51960.12990000000000002Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGATTAATAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTG51880.1297Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTTCTACAGTCCGACGATCAAACAGATCGGAAGAGCACACGTCTGAACT50310.125775Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTCTGA49890.124725Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCTTGCTACCCGGGGCCGCGGGCAAGATCGGAAGAGCACACGTCTGAA49680.1242Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGAAACTGCGAATGGCTCATTAAATCAGAGATCGGAAGAGCACACGTCTG48320.1208Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAACTCTTAGCGGTGGATCACTCGGCTCGAGATCGGAAGAGCACACGTCT47750.119375Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCCTCACTTACCCGGTTGGGCAGGGAGATCGGAAGAGCACACGTCTGA47540.11885000000000001Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGAACCCCGCCCGTGATGGGGACCGGGGATTAGATCGGAAGAGCACAC46880.11720000000000001No Hit
CCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGTCT46730.116825Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACCCCGCCCGTGATGGGGACCGGGGATTAGATCGGAAGAGCACACGTCT45880.1147Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGTC45730.114325No Hit
GTTGAACCCCGCCCGTGATGGGGACCGGGGATAGATCGGAAGAGCACACG44940.11234999999999999No Hit
AAACGATGCCGACCCGCGATCCGGCGGCGAGATCGGAAGAGCACACGTCT44220.11055Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTCGCCGCTTGCTCTCCGGGGCCGCGGGTAGATCGGAAGAGCACACGTC43890.10972499999999999No Hit
CATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCTG42840.10709999999999999Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGAGGGAAGAGCCCAGCGCCGAATCCCCAGATCGGAAGAGCACACGTCT42080.1052Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGTC41550.103875No Hit
ACTTCGCCGCTTGCTCTCCGGGGCCGCGGGTGAGATCGGAAGAGCACACG41310.103275No Hit
ACCTTTCGAACGCACATTGCGGCCCCGGAGATCGGAAGAGCACACGTCTG40900.10225Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACACTTCGAACGCACTTTGCGGCCCCGGGAGATCGGAAGAGCACACGTCT40850.10212500000000001Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTTCGGGGGCGAAGGTGGCTCGCGGCTAGATCGGAAGAGCACACGTCTG40770.101925Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)