FastQCFastQC Report
Thu 28 Sept 2023
SRR1062524.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1062524.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Total Bases304 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCGCCTGGGAATACCAGGTGCTGTAAGAGATCGGAAGAGCACACGTCTG1739614.349025Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG1698954.247375TruSeq Adapter, Index 10 (100% over 49bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGT407271.018175No Hit
ATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTCTG390320.9757999999999999Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGCCTACGTCCTCCTAAGCGCAGACCGAGATCGGAAGAGCACACGTCT363800.9095000000000001Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGCCTGGGAATACCAGGTGCTGTAAAGATCGGAAGAGCACACGTCTGA299630.749075Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTCGGTCGGTGCGGAGAGCCAGCCGTGAGATCGGAAGAGCACACGTCTG241810.604525Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCTTTCGAACGCACATTGCGGCCCCGGGTAGATCGGAAGAGCACACGTC227910.5697749999999999No Hit
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGTC182630.45657500000000006No Hit
AACTCTTAGCGGTGGATCACTCGGCTCGAGATCGGAAGAGCACACGTCTG163860.40965Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCT162050.405125Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTC157840.3946No Hit
ACCTTTCGAACGCACATTGCGGCCCCGGGAGATCGGAAGAGCACACGTCT156400.391Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTTCGCCGCTTGCTCTCCGGGGCCGCGGGTAGATCGGAAGAGCACACGT154860.38715No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACG152440.3811No Hit
CCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGTCT146130.36532499999999996Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGTCTG138860.34715Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACG138160.3454No Hit
AACTCTTAGCGGTGGATCACTCGGAGATCGGAAGAGCACACGTCTGAACT118340.29585Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGT117750.294375No Hit
CCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCTG116560.2914Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACCGCCTGGGAATACCAGGTGCTGTAAGAGATCGGAAGAGCACACGTCT115610.289025Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGTCT112080.2802Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACTCTTAGCGGTGGATCACTCGGCTCGTGAGATCGGAAGAGCACACGTC112030.28007499999999996No Hit
ACACTTCGAACGCACTTTGCGGCCCCGGAGATCGGAAGAGCACACGTCTG111990.27997500000000003Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGT109930.274825No Hit
AGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGA107560.2689Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGTCTG106900.26725Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTC102910.257275No Hit
CCTTTCGAACGCACATTGCGGCCCCGGGAGATCGGAAGAGCACACGTCTG99810.249525Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACGTC99800.24949999999999997No Hit
GGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGAAC98880.24719999999999998Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGT98600.24650000000000002No Hit
TACCTGGTTGATCCTGCCAGTAACAGATCGGAAGAGCACACGTCTGAACT94370.23592499999999997Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGCGGGGGGCGCACCACCGGCCCGTCTCGTAGATCGGAAGAGCACACGT90260.22565No Hit
CCGCCTGGGAATACCAGGTGCTGTAAGAGATCGGAAGAGCACACGTCTGA89050.222625Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGTC87020.21755No Hit
ACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGAACTC85010.212525Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTCT80850.202125Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCGGTCGGTGCGGAGAGCCAGCCGAGATCGGAAGAGCACACGTCTGAA80750.20187500000000003Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTTCGAACGCACTTTGCGGCCCCGGAGATCGGAAGAGCACACGTCTGAA80080.2002Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGGGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGA77460.19365000000000002Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTG75070.18767499999999998Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGAACT71340.17834999999999998Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCCATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTC70190.175475No Hit
GCCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCT69650.174125Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGACCGCCTGGGAATACCAGGTGCTGTAAGAGATCGGAAGAGCACACGTC67600.169No Hit
AAGGGTCAGGGGGCGAAGGTGGCCCGTCGGAGATCGGAAGAGCACACGTC65110.162775No Hit
TCTCTCGGCGCGGGCCGGGGGGCCAAAAGATCGGAAGAGCACACGTCTGA64800.16199999999999998Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGCGAGAACTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTGAA63860.15965000000000001Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCTCGGTCGGTGCGGAGAGCCAGCCGTAGATCGGAAGAGCACACGTCTGA62390.155975Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGCCTGGGAATACCAGGTGCTGAGATCGGAAGAGCACACGTCTGAACT60670.151675Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCTGA60110.150275Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGCCTGGGAATACCAGGTGCTGTAAGATCGGAAGAGCACACGTCTGAA59140.14785Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGAGAAGACGATCGAACTCGGTCGTTTAAGATCGGAAGAGCACACGTCT58460.14615Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGAACCCCGCCCGTGATGGGGACCGGGGATTAGATCGGAAGAGCACACG57310.14327499999999999No Hit
CGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGTCTGAA55310.138275Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTC54310.135775No Hit
CCGCTTGCTACCCGGGGCCGCGGGCAAGATCGGAAGAGCACACGTCTGAA53720.1343Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGATTAATAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTG51590.128975Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGTCT51130.12782500000000002Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTTCTACAGTCCGACGATCAAACAGATCGGAAGAGCACACGTCTGAACT50150.12537500000000001Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGTC49660.12415No Hit
TCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTCTGA48620.12155Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACCCCGCCCGTGATGGGGACCGGGGATTAGATCGGAAGAGCACACGTCT48440.1211Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCCTCACTTACCCGGTTGGGCAGGGAGATCGGAAGAGCACACGTCTGA48390.12097500000000001Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAACTCTTAGCGGTGGATCACTCGGCTCGAGATCGGAAGAGCACACGTCT48210.120525Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAAACTGCGAATGGCTCATTAAATCAGAGATCGGAAGAGCACACGTCTG47060.11765Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTGAACCCCGCCCGTGATGGGGACCGGGGATTAGATCGGAAGAGCACAC46720.1168No Hit
CGCCCCCTCGCCCCGACTTCGCCGCTTGCAGATCGGAAGAGCACACGTCT45140.11284999999999999Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTCGCCGCTTGCTCTCCGGGGCCGCGGGTAGATCGGAAGAGCACACGTC44780.11195000000000001No Hit
GTTGAACCCCGCCCGTGATGGGGACCGGGGATAGATCGGAAGAGCACACG44770.111925No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGTC44760.1119No Hit
ACTTCGCCGCTTGCTCTCCGGGGCCGCGGGTGAGATCGGAAGAGCACACG44260.11065000000000001No Hit
AAACGATGCCGACCCGCGATCCGGCGGCGAGATCGGAAGAGCACACGTCT43800.1095Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCTG43750.10937500000000001Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGAGGGAAGAGCCCAGCGCCGAATCCCCAGATCGGAAGAGCACACGTCT42550.106375Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGCGGGGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC42380.10594999999999999No Hit
GGTTCGGGGGCGAAGGTGGCTCGCGGCTAGATCGGAAGAGCACACGTCTG42310.10577500000000001Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCTTTCGAACGCACATTGCGGCCCCGGAGATCGGAAGAGCACACGTCTG42130.10532499999999999Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCCTACGTCCTCCTAAGCGCAGACCGAGATCGGAAGAGCACACGTCTGA42060.10515Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGAGAAGACGATCGAACTCGGTCGTTTAAGATCGGAAGAGCACACGT41410.10352499999999999No Hit
GCGGGGGGCGCACCACCGGCCCGTCTCGTAGATCGGAAGAGCACACGTCT41410.10352499999999999Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)